##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AspI
AvrII BalI BbrPI BcgI BclI
BfrI BlnI BsaAI BseAI BsmI
BspDI BspEI BspLU11I BstBI BstEII
BstPI Bsu36I ClaI DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoT22I FbaI
KpnI MluNI MroI MscI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmlI Psp1406I PvuI
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 3262 GTATAC GT^[AC][GT]AC
AflIII 491 ACGCGT A^C[GA][CT]GT
AseI 1513 ATTAAT AT^TAAT
AsnI 1513 ATTAAT AT^TAAT
BbeI 53 GGCGCC GGCGC^C
BbvCI 1268 CCTCAGC CCTCAGC(-5/-2)
BciVI 943 GTATCC GTATCC(6/5)
BlpI 783 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 783 GCTCAGC GC^T[ACGT]AGC
BsaWI 1406 ACCGGA [AT]^CCGG[AT]
BsiWI 344 CGTACG C^GTACG
BsmBI 4019 GAGACG CGTCTC(1/5)
Bst1107I 3262 GTATAC GTA^TAC
BstZ17I 3262 GTATAC GTA^TAC
CelII 783 GCTCAGC GC^T[ACGT]AGC
CpoI 1813 CGGACCG CG^G[AT]CCG
EcoRV 2320 GATATC GAT^ATC
FseI 892 GGCCGGCC GGCCGG^CC
HindIII 1061 AAGCTT A^AGCTT
HpaI 3815 GTTAAC GTT^AAC
KasI 53 GGCGCC G^GCGCC
MfeI 3533 CAATTG C^AATTG
MluI 491 ACGCGT A^CGCGT
MunI 3533 CAATTG C^AATTG
NarI 53 GGCGCC GG^CGCC
NcoI 95 CCATGG C^CATGG
NdeI 3747 CATATG CA^TATG
NheI 1714 GCTAGC G^CTAGC
PmeI 3622 GTTTAAAC GTTT^AAAC
PshAI 840 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1513 ATTAAT AT^TAAT
RsrII 1813 CGGACCG CG^G[AT]CCG
SexAI 3038 ACCTGGT A^CC[AT]GGT
SfiI 693 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 53 GGCGCC GGC^GCC
SmaI 77 CCCGGG CCC^GGG
XmaCI 77 CCCGGG C^CCGGG
XmaI 77 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Asp700I 747 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3189 GAAAATCTTC
AviII 2171 TGCGCA TGC^GCA
2300 TGCGCA
BamHI 1239 GGATCC G^GATCC
2006 GGATCC
BanI 53 GGCGCC G^G[CT][GA]CC
2548 GGCACC
BglI 521 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
694 GCCCAGCTGGC
BglII 2411 AGATCT A^GATCT
3586 AGATCT
BsaI 1496 GAGACC GGTCTC(1/5)
3326 GAGACC
Bsp1407I 3315 TGTACA T^GTACA
3998 TGTACA
BsrDI 1464 CATTGC GCAATG(2/0)
1586 CATTGC
BsrGI 3315 TGTACA T^GTACA
3998 TGTACA
EcoNI 3039 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3882 CCTGGTTAAGG
FspI 2171 TGCGCA TGC^GCA
2300 TGCGCA
KspI 264 CCGCGG CCGC^GG
704 CCGCGG
PaeR7I 400 CTCGAG C^TCGAG
2358 CTCGAG
SacII 264 CCGCGG CCGC^GG
704 CCGCGG
SspBI 3315 TGTACA T^GTACA
3998 TGTACA
StuI 984 AGGCCT AGG^CCT
1600 AGGCCT
XcmI 2588 CCAACCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2648 CCAGCCAGAAGCTGG
XhoI 400 CTCGAG C^TCGAG
2358 CTCGAG
XmnI 747 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3189 GAAAATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApoI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrI BssHII
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII