##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI AhdI Alw44I ApaLI AscI
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BfrI BlnI BsaHI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoO65I
FseI FspI Hin1I HpaI KasI
KspI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SapI SfiI SfoI SgrAI
SmaI SpeI SphI SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 6989 GGTACC G^GTACC
AflIII 5414 ACATGT A^C[GA][CT]GT
Aor51HI 6482 AGCGCT AGC^GCT
AseI 4985 ATTAAT AT^TAAT
AsnI 4985 ATTAAT AT^TAAT
Asp718I 6989 GGTACC G^GTACC
AvaI 856 CTCGAG C^[CT]CG[GA]G
BclI 2195 TGATCA T^GATCA
BglII 4473 AGATCT A^GATCT
BlpI 6127 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 6127 GCTCAGC GC^T[ACGT]AGC
BsaAI 6883 TACGTA [CT]AC^GT[GA]
BsaWI 4587 TCCGGT [AT]^CCGG[AT]
BsoBI 856 CTCGAG C^[CT]CG[GA]G
BspLU11I 5414 ACATGT A^CATGT
BssSI 3343 CACGAG CACGAG(-5/-1)
BstBI 1144 TTCGAA TT^CGAA
CelII 6127 GCTCAGC GC^T[ACGT]AGC
DrdI 3008 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 6482 AGCGCT AGC^GCT
EcoNI 4974 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3845 GAATTC G^AATTC
EcoRV 3081 GATATC GAT^ATC
FbaI 2195 TGATCA T^GATCA
HgaI 1481 GCGTC GACGC(5/10)
KpnI 6989 GGTACC GGTAC^C
MfeI 3898 CAATTG C^AATTG
MslI 1026 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3898 CAATTG C^AATTG
NcoI 6507 CCATGG C^CATGG
NspV 1144 TTCGAA TT^CGAA
PaeR7I 856 CTCGAG C^TCGAG
PshAI 4248 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4985 ATTAAT AT^TAAT
SbfI 1986 CCTGCAGG CCTGCA^GG
SfuI 1144 TTCGAA TT^CGAA
SnaBI 6883 TACGTA TAC^GTA
Sse8387I 1986 CCTGCAGG CCTGCA^GG
XcmI 6638 CCAGTTTTTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 856 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3563 GTCTAC GT^[AC][GT]AC
5187 GTCTAC
ApaI 191 GGGCCC GGGCC^C
6751 GGGCCC
BalI 5356 TGGCCA TGG^CCA
5990 TGGCCA
BamHI 1271 GGATCC G^GATCC
2419 GGATCC
BanI 6982 GGTGCC G^G[CT][GA]CC
6989 GGTACC
BcnI 5135 CCGGG CC^[GC]GG
7252 CCCGG
BglI 1469 GCCTCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5836 GCCAAAGGGGC
BspHI 1025 TCATGA T^CATGA
5392 TCATGA
CfoI 5328 GCGC GCG^C
6483 GCGC
DraI 6972 TTTAAA TTT^AAA
7058 TTTAAA
DraIII 943 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4795 CACCAAGTG
DsaI 3670 CCGTGG C^C[GA][CT]GG
6507 CCATGG
EaeI 5356 TGGCCA [CT]^GGCC[GA]
5990 TGGCCA
FauI 992 GCGGG CCCGC(4/6)
5797 GCGGG
HaeII 5327 AGCGCC [GA]GCGC^[CT]
6482 AGCGCT
HhaI 5328 GCGC GCG^C
6483 GCGC
HinP1I 5328 GCGC G^CGC
6483 GCGC
HincII 2004 GTTGAC GT[CT]^[GA]AC
7232 GTCAAC
HindII 2004 GTTGAC GT[CT]^[GA]AC
7232 GTCAAC
MluNI 5356 TGGCCA TGG^CCA
5990 TGGCCA
MscI 5356 TGGCCA TGG^CCA
5990 TGGCCA
NciI 5135 CCGGG CC^[GC]GG
7252 CCCGG
NspI 4274 ACATGC [GA]CATG^[CT]
5414 ACATGT
RcaI 1025 TCATGA T^CATGA
5392 TCATGA
ScaI 543 AGTACT AGT^ACT
6831 AGTACT
XbaI 6931 TCTAGA T^CTAGA
7304 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AclI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaII BanII BbsI BbvCI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII EarI Eco81I EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
PflMI PleI PpuMI Psp1406I PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI