##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I ApaI ApaLI
AscI Asp718I AspHI AviII AvrII
BamHI BanII BbeI BbrPI BfrI
BglI BlnI BsaAI BsaHI BseAI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoT22I
FseI FspI Hin1I HpaI KasI
KpnI MluI MroI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 2995 ACATGT A^C[GA][CT]GT
AocI 3392 CCTCAGG CC^T[ACGT]AGG
Aor51HI 17 AGCGCT AGC^GCT
AspI 3183 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 3544 GTATCC GTATCC(6/5)
BclI 2186 TGATCA T^GATCA
BcnI 3321 CCCGG CC^[GC]GG
BglII 1625 AGATCT A^GATCT
BlpI 1662 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1662 GCTAAGC GC^T[ACGT]AGC
BsaBI 2712 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1614 GAGACC GGTCTC(1/5)
BsaWI 2873 TCCGGT [AT]^CCGG[AT]
BsgI 1575 GTGCAG GTGCAG(16/14)
BspLU11I 2995 ACATGT A^CATGT
Bsu36I 3392 CCTCAGG CC^T[ACGT]AGG
CelII 1662 GCTAAGC GC^T[ACGT]AGC
Eco47III 17 AGCGCT AGC^GCT
Eco81I 3392 CCTCAGG CC^T[ACGT]AGG
EcoNI 1437 CCTAAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 936 GAATTC G^AATTC
FbaI 2186 TGATCA T^GATCA
HgaI 47 GACGC GACGC(5/10)
HindIII 2233 AAGCTT A^AGCTT
KspI 139 CCGCGG CCGC^GG
MamI 2712 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3490 CAATTG C^AATTG
MunI 3490 CAATTG C^AATTG
NciI 3321 CCCGG CC^[GC]GG
PflFI 3183 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 139 CCGCGG CCGC^GG
SalI 1290 GTCGAC G^TCGAC
SapI 2255 GAAGAGC GCTCTTC(1/4)
Tth111I 3183 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1122 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1290 GTCGAC GT^[AC][GT]AC
1376 GTCTAC
AccII 49 CGCG CG^CG
140 CGCG
AhdI 1593 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2518 GACCAGCTGTC
AseI 1230 ATTAAT AT^TAAT
3926 ATTAAT
AsnI 1230 ATTAAT AT^TAAT
3926 ATTAAT
AspEI 1593 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2518 GACCAGCTGTC
BanI 1466 GGCACC G^G[CT][GA]CC
3305 GGCACC
BcgI 1718 CGAAGCATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3265 CGATTCTCCTGC
BmyI 2987 GTGCCC G[AGT]GC[ACT]^C
3964 GGGCAC
Bsp1286I 2987 GTGCCC G[AGT]GC[ACT]^C
3964 GGGCAC
BspHI 965 TCATGA T^CATGA
4183 TCATGA
BsrDI 511 CATTGC GCAATG(2/0)
4228 GCAATG
BstBI 220 TTCGAA TT^CGAA
1364 TTCGAA
BstUI 49 CGCG CG^CG
140 CGCG
CfoI 18 GCGC GCG^C
3452 GCGC
DsaI 139 CCGCGG C^C[GA][CT]GG
151 CCGTGG
Eam1105I 1593 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2518 GACCAGCTGTC
EcoRV 2291 GATATC GAT^ATC
3859 GATATC
EcoT14I 287 CCTTGG C^C[AT][AT]GG
314 CCAAGG
HaeII 17 AGCGCT [GA]GCGC^[CT]
3451 AGCGCC
HhaI 18 GCGC GCG^C
3452 GCGC
HinP1I 18 GCGC G^CGC
3452 GCGC
HincII 1290 GTCGAC GT[CT]^[GA]AC
2526 GTCAAC
HindII 1290 GTCGAC GT[CT]^[GA]AC
2526 GTCAAC
MaeII 1418 ACGT A^CGT
2239 ACGT
MvnI 49 CGCG CG^CG
140 CGCG
NspV 220 TTCGAA TT^CGAA
1364 TTCGAA
PaeR7I 1611 CTCGAG C^TCGAG
3285 CTCGAG
PpuMI 134 GGGTCCC [GA]G^G[AT]CC[CT]
2726 AGGTCCT
PshBI 1230 ATTAAT AT^TAAT
3926 ATTAAT
PvuII 2521 CAGCTG CAG^CTG
4210 CAGCTG
RcaI 965 TCATGA T^CATGA
4183 TCATGA
SexAI 2458 ACCTGGT A^CC[AT]GGT
3915 ACCTGGT
SfuI 220 TTCGAA TT^CGAA
1364 TTCGAA
SspI 681 AATATT AAT^ATT
3468 AATATT
StyI 287 CCTTGG C^C[AT][AT]GG
314 CCAAGG
XhoI 1611 CTCGAG C^TCGAG
3285 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaI AvaII
BalI BbsI BbvCI BbvI BfaI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1407I BspMI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI