##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
Aor51HI AscI Asp700I Asp718I BbrPI
BciVI BclI BglII BsaBI BsaI
BsiEI BsiWI BsmI BspLU11I BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FbaI
FseI HincII HindII HpaI KpnI
MamI MfeI MluI MunI NotI
NspV PacI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacI SalI SbfI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 2213 TCCGGA T^CCGGA
AflII 3084 CTTAAG C^TTAAG
AhdI 218 GACCGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1437 GTGCAC G^TGCAC
AlwNI 2609 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2010 CCTCAGG CC^T[ACGT]AGG
ApaI 3100 GGGCCC GGGCC^C
ApaLI 1437 GTGCAC G^TGCAC
AspEI 218 GACCGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1437 GTGCAC G[AT]GC[AT]^C
AspI 122 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2924 CCTAGG C^CTAGG
BbsI 1082 GTCTTC GAAGAC(2/6)
BbvCI 1175 GCTGAGG CCTCAGC(-5/-2)
BfrI 3084 CTTAAG C^TTAAG
BlnI 2924 CCTAGG C^CTAGG
BpuAI 1082 GTCTTC GAAGAC(2/6)
BsaWI 2213 TCCGGA [AT]^CCGG[AT]
BseAI 2213 TCCGGA T^CCGGA
BsiHKAI 1437 GTGCAC G[AT]GC[AT]^C
BspEI 2213 TCCGGA T^CCGGA
BssSI 27 CTCGTG CACGAG(-5/-1)
BstXI 2675 CCATGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2010 CCTCAGG CC^T[ACGT]AGG
Eam1105I 218 GACCGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2010 CCTCAGG CC^T[ACGT]AGG
EcoT22I 3590 ATGCAT ATGCA^T
MroI 2213 TCCGGA T^CCGGA
NaeI 19 GCCGGC GCC^GGC
NgoMIV 19 GCCGGC G^CCGGC
NheI 2288 GCTAGC G^CTAGC
NruI 616 TCGCGA TCG^CGA
NsiI 3590 ATGCAT ATGCA^T
PaeR7I 371 CTCGAG C^TCGAG
PflFI 122 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1624 GCTCTTC GCTCTTC(1/4)
SfiI 514 GGCCTTCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 122 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1819 TCTAGA T^CTAGA
XhoI 371 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AseI 3041 ATTAAT AT^TAAT
3276 ATTAAT
AsnI 3041 ATTAAT AT^TAAT
3276 ATTAAT
AviII 240 TGCGCA TGC^GCA
1467 TGCGCA
BamHI 591 GGATCC G^GATCC
2388 GGATCC
BglI 515 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1065 GCCTGCTGGGC
BsaAI 1152 TACGTG [CT]AC^GT[GA]
3036 CACGTA
BsmBI 2396 CGTCTC CGTCTC(1/5)
722 GAGACG
Bsp1407I 1383 TGTACA T^GTACA
3064 TGTACA
BspDI 866 ATCGAT AT^CGAT
3594 ATCGAT
BsrFI 19 GCCGGC [GA]^CCGG[CT]
439 GCCGGT
BsrGI 1383 TGTACA T^GTACA
3064 TGTACA
BssHII 300 GCGCGC G^CGCGC
302 GCGCGC
BstAPI 427 GCACCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
490 GCATGCACTGC
BstYI 591 GGATCC [GA]^GATC[CT]
2388 GGATCC
Cfr10I 19 GCCGGC [GA]^CCGG[CT]
439 GCCGGT
ClaI 866 ATCGAT AT^CGAT
3594 ATCGAT
EcoNI 1786 CCTATTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2956 CCTGACCAAGG
FspI 240 TGCGCA TGC^GCA
1467 TGCGCA
HindIII 611 AAGCTT A^AGCTT
4167 AAGCTT
KspI 157 CCGCGG CCGC^GG
346 CCGCGG
MflI 591 GGATCC [GA]^GATC[CT]
2388 GGATCC
NdeI 3683 CATATG CA^TATG
3734 CATATG
PflMI 2887 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3553 CCATTGTATGG
PshBI 3041 ATTAAT AT^TAAT
3276 ATTAAT
SacII 157 CCGCGG CCGC^GG
346 CCGCGG
ScaI 2462 AGTACT AGT^ACT
3570 AGTACT
SexAI 264 ACCTGGT A^CC[AT]GGT
3176 ACCTGGT
SspBI 1383 TGTACA T^GTACA
3064 TGTACA
SspI 2911 AATATT AAT^ATT
3547 AATATT
TfiI 932 GAATC G^A[AT]TC
1781 GAATC
Van91I 2887 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3553 CCATTGTATGG
XcmI 1281 CCACGTCGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1393 CCACGCCCGCTTTGG
XhoII 591 GGATCC [GA]^GATC[CT]
2388 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcgI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SphI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmaCI XmaI