##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AclI AflII AocI AseI AsnI
BbvCI BciVI BfrI BglII BlpI
Bpu1102I BsaBI BsiWI BspHI BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII CpoI DraI Eco81I
EcoO65I EcoRI EcoRV EcoT22I HincII
HindII HindIII HpaI MamI MfeI
MluI MunI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2792 GACGTC GACGT^C
Acc65I 840 GGTACC G^GTACC
AccI 2513 GTAGAC GT^[AC][GT]AC
AgeI 837 ACCGGT A^CCGGT
AscI 65 GGCGCGCC GG^CGCGCC
Asp700I 2566 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 840 GGTACC G^GTACC
AspI 13 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1820 TGCGCA TGC^GCA
AvrII 219 CCTAGG C^CTAGG
BbrPI 3061 CACGTG CAC^GTG
BclI 933 TGATCA T^GATCA
BlnI 219 CCTAGG C^CTAGG
Bsp1407I 3553 TGTACA T^GTACA
BspLU11I 3711 ACATGT A^CATGT
BsrGI 3553 TGTACA T^GTACA
BssSI 1072 CACGAG CACGAG(-5/-1)
BstXI 972 CCATGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2141 GACGCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1852 CCTATCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 933 TGATCA T^GATCA
FseI 275 GGCCGGCC GGCCGG^CC
FspI 1820 TGCGCA TGC^GCA
KpnI 840 GGTACC GGTAC^C
NdeI 855 CATATG CA^TATG
NheI 2461 GCTAGC G^CTAGC
PflFI 13 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 837 ACCGGT A^CCGGT
PmaCI 3061 CACGTG CAC^GTG
PmlI 3061 CACGTG CAC^GTG
SapI 2569 GCTCTTC GCTCTTC(1/4)
SmlI 3159 CTCAAG C^T[CT][GA]AG
SphI 1623 GCATGC GCATG^C
SspBI 3553 TGTACA T^GTACA
Tth111I 13 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2566 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 874 TCCGGA T^CCGGA
2256 TCCGGA
AflIII 1199 ACGTGT A^C[GA][CT]GT
3711 ACATGT
AhdI 760 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2033 GACGGCCTGTC
Aor51HI 624 AGCGCT AGC^GCT
2898 AGCGCT
AspEI 760 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2033 GACGGCCTGTC
BamHI 2358 GGATCC G^GATCC
2544 GGATCC
BbsI 998 GTCTTC GAAGAC(2/6)
1232 GTCTTC
BcgI 1195 CGAGACGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3669 CGATGTGATTGC
BpuAI 998 GTCTTC GAAGAC(2/6)
1232 GTCTTC
BsaAI 1186 CACGTA [CT]AC^GT[GA]
3061 CACGTG
BseAI 874 TCCGGA T^CCGGA
2256 TCCGGA
BsgI 565 CTGCAC GTGCAG(16/14)
2590 CTGCAC
BsmBI 1196 GAGACG CGTCTC(1/5)
2712 GAGACG
BsmI 2348 GAATGC GAATGC(1/-1)
3038 GCATTC
BspDI 1139 ATCGAT AT^CGAT
1454 ATCGAT
BspEI 874 TCCGGA T^CCGGA
2256 TCCGGA
BstAPI 2343 GCATCGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2614 GCACCTGCTGC
ClaI 1139 ATCGAT AT^CGAT
1454 ATCGAT
Eam1105I 760 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2033 GACGGCCTGTC
Eco47III 624 AGCGCT AGC^GCT
2898 AGCGCT
MroI 874 TCCGGA T^CCGGA
2256 TCCGGA
NcoI 302 CCATGG C^CATGG
2238 CCATGG
PflMI 2167 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2278 CCACTGTGTGG
PshAI 3279 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3818 GACTCCGGTC
PvuII 587 CAGCTG CAG^CTG
1280 CAGCTG
SgrAI 455 CGCCGGCG C[GA]^CCGG[CT]G
3654 CGCCGGTG
StuI 1564 AGGCCT AGG^CCT
2844 AGGCCT
Van91I 2167 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2278 CCACTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI