##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AviII AvrII BbeI BbrPI
BlnI BseAI BsiEI BsiWI Bsp1407I
BspEI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Cfr10I CpoI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FauI FseI FspI HindIII
HpaI KasI KpnI KspI MluI
MroI NaeI NarI NcoI NgoMIV
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SapI SbfI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AcyI 1072 GGCGTC G[GA]^CG[CT]C
AflII 4064 CTTAAG C^TTAAG
AflIII 2729 ACATGT A^C[GA][CT]GT
AhdI 3760 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3927 GTGCAC G^TGCAC
AlwNI 3854 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1702 CCTAAGG CC^T[ACGT]AGG
ApaLI 3927 GTGCAC G^TGCAC
AspEI 3760 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3939 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1870 TGGCCA TGG^CCA
BamHI 3951 GGATCC G^GATCC
BbvCI 1272 CCTCAGC CCTCAGC(-5/-2)
BciVI 2934 GGATAC GTATCC(6/5)
BclI 3267 TGATCA T^GATCA
BcnI 1785 CCCGG CC^[GC]GG
BfrI 4064 CTTAAG C^TTAAG
BsaAI 3877 CACGTA [CT]AC^GT[GA]
BsaBI 1388 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1072 GGCGTC G[GA]^CG[CT]C
BsaWI 3276 ACCGGA [AT]^CCGG[AT]
BspDI 1582 ATCGAT AT^CGAT
BspHI 367 TCATGA T^CATGA
BspLU11I 2729 ACATGT A^CATGT
BstXI 1681 CCACAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1702 CCTAAGG CC^T[ACGT]AGG
ClaI 1582 ATCGAT AT^CGAT
DraI 2811 TTTAAA TTT^AAA
DraIII 411 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 698 CCGTGG C^C[GA][CT]GG
EaeI 1870 TGGCCA [CT]^GGCC[GA]
Eam1105I 3760 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1702 CCTAAGG CC^T[ACGT]AGG
EcoRV 2088 GATATC GAT^ATC
EcoT14I 3550 CCAAGG C^C[AT][AT]GG
FbaI 3267 TGATCA T^GATCA
HaeII 115 AGCGCC [GA]GCGC^[CT]
Hin1I 1072 GGCGTC G[GA]^CG[CT]C
HincII 1515 GTCGAC GT[CT]^[GA]AC
HindII 1515 GTCGAC GT[CT]^[GA]AC
MamI 1388 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 745 CAATTG C^AATTG
MluNI 1870 TGGCCA TGG^CCA
MscI 1870 TGGCCA TGG^CCA
MunI 745 CAATTG C^AATTG
NciI 1785 CCCGG CC^[GC]GG
NdeI 1908 CATATG CA^TATG
NheI 2322 GCTAGC G^CTAGC
PflFI 3939 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 367 TCATGA T^CATGA
SacI 1203 GAGCTC GAGCT^C
SalI 1515 GTCGAC G^TCGAC
SexAI 1058 ACCAGGT A^CC[AT]GGT
SfiI 2634 GGCCAGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 2642 AGGCCT AGG^CCT
StyI 3550 CCAAGG C^C[AT][AT]GG
Tth111I 3939 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1928 CCATCTGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Asp700I 987 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3090 GAAAACCTTC
BcgI 113 CGAGCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2023 GCAGCTGTATCG
BglI 1277 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2635 GCCAGAGAGGC
BglII 1255 AGATCT A^GATCT
1969 AGATCT
BlpI 1664 GCTCAGC GC^T[ACGT]AGC
3321 GCTGAGC
Bpu1102I 1664 GCTCAGC GC^T[ACGT]AGC
3321 GCTGAGC
BsaI 1168 GGTCTC GGTCTC(1/5)
2686 GGTCTC
BsgI 2665 CTGCAC GTGCAG(16/14)
3826 GTGCAG
BsmBI 31 GAGACG CGTCTC(1/5)
46 GAGACG
BsmI 3912 GAATGC GAATGC(1/-1)
3579 GCATTC
BspMI 2063 ACCTGC ACCTGC(4/8)
3167 ACCTGC
CelII 1664 GCTCAGC GC^T[ACGT]AGC
3321 GCTGAGC
CfoI 36 GCGC GCG^C
116 GCGC
DraII 2978 GGGTCCC [GA]G^G[ACGT]CC[CT]
3943 AGGTCCC
EarI 1692 GAAGAG CTCTTC(1/4)
3217 GAAGAG
EcoO109I 2978 GGGTCCC [GA]G^G[ACGT]CC[CT]
3943 AGGTCCC
EcoRI 1418 GAATTC G^AATTC
2174 GAATTC
HapII 1786 CCGG C^CGG
3277 CCGG
HgaI 33 GACGC GACGC(5/10)
1073 GCGTC
HhaI 36 GCGC GCG^C
116 GCGC
HinP1I 36 GCGC G^CGC
116 GCGC
HpaII 1786 CCGG C^CGG
3277 CCGG
Ksp632I 1692 GAAGAG CTCTTC(1/4)
3217 GAAGAG
MaeII 3287 ACGT A^CGT
3878 ACGT
MspI 1786 CCGG C^CGG
3277 CCGG
PaeR7I 111 CTCGAG C^TCGAG
3018 CTCGAG
PpuMI 2978 GGGTCCC [GA]G^G[AT]CC[CT]
3943 AGGTCCC
ScaI 482 AGTACT AGT^ACT
1403 AGTACT
SpeI 1865 ACTAGT A^CTAGT
3475 ACTAGT
XhoI 111 CTCGAG C^TCGAG
3018 CTCGAG
XmnI 987 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3090 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I Cfr13I DdeI DpnII EcoNI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII