##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AgeI Aor51HI AscI
Asp718I AspI AvrII BbeI BbrPI
BcgI BglI BlnI BlpI Bpu1102I
BsaAI BsiWI Bsp1407I BspDI BspHI
BsrFI BsrGI BssHII BstEII BstPI
CelII Cfr10I ClaI Eco47III EcoNI
EcoO65I EcoRI EcoRV FseI HincII
HindII HpaI KasI KpnI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PvuI RcaI SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SwaI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AclI 2408 AACGTT AA^CGTT
AcyI 696 GACGCC G[GA]^CG[CT]C
AflII 4284 CTTAAG C^TTAAG
AflIII 800 ACATGT A^C[GA][CT]GT
Alw44I 1846 GTGCAC G^TGCAC
AocI 717 CCTCAGG CC^T[ACGT]AGG
ApaLI 1846 GTGCAC G^TGCAC
AseI 3962 ATTAAT AT^TAAT
AsnI 3962 ATTAAT AT^TAAT
AviII 48 TGCGCA TGC^GCA
BfrI 4284 CTTAAG C^TTAAG
BglII 4599 AGATCT A^GATCT
BsaBI 4937 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 696 GACGCC G[GA]^CG[CT]C
BsmBI 1747 GAGACG CGTCTC(1/5)
BsmI 228 GAATGC GAATGC(1/-1)
BspLU11I 800 ACATGT A^CATGT
BssSI 1577 CTCGTG CACGAG(-5/-1)
Bst1107I 3518 GTATAC GTA^TAC
BstBI 3233 TTCGAA TT^CGAA
BstXI 1583 CCAGTGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3518 GTATAC GTA^TAC
Bsu36I 717 CCTCAGG CC^T[ACGT]AGG
CpoI 993 CGGTCCG CG^G[AT]CCG
DraI 3597 TTTAAA TTT^AAA
DraIII 2837 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1723 GACGGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 10 CGGCCG C^GGCCG
EclXI 10 CGGCCG C^GGCCG
Eco52I 10 CGGCCG C^GGCCG
Eco81I 717 CCTCAGG CC^T[ACGT]AGG
EcoT22I 3768 ATGCAT ATGCA^T
FspI 48 TGCGCA TGC^GCA
HaeII 26 GGCGCT [GA]GCGC^[CT]
Hin1I 696 GACGCC G[GA]^CG[CT]C
HindIII 3317 AAGCTT A^AGCTT
KspI 1064 CCGCGG CCGC^GG
MamI 4937 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 4814 GCTAGC G^CTAGC
NsiI 3768 ATGCAT ATGCA^T
NspV 3233 TTCGAA TT^CGAA
PaeR7I 4163 CTCGAG C^TCGAG
PpuMI 2357 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 3962 ATTAAT AT^TAAT
Psp1406I 2408 AACGTT AA^CGTT
RsrII 993 CGGTCCG CG^G[AT]CCG
SacII 1064 CCGCGG CCGC^GG
SfuI 3233 TTCGAA TT^CGAA
SpeI 3838 ACTAGT A^CTAGT
StuI 4142 AGGCCT AGG^CCT
XhoI 4163 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 3322 TCCGGA T^CCGGA
4473 TCCGGA
AhdI 2572 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3733 GACACACGGTC
ApaI 1085 GGGCCC GGGCC^C
2831 GGGCCC
AspEI 2572 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3733 GACACACGGTC
BalI 1838 TGGCCA TGG^CCA
4557 TGGCCA
BamHI 1376 GGATCC G^GATCC
2868 GGATCC
BanI 1794 GGCACC G^G[CT][GA]CC
4224 GGCACC
BbvCI 4882 CCTCAGC CCTCAGC(-5/-2)
1122 GCTGAGG
BclI 3131 TGATCA T^GATCA
4377 TGATCA
BsaWI 3322 TCCGGA [AT]^CCGG[AT]
4473 TCCGGA
BseAI 3322 TCCGGA T^CCGGA
4473 TCCGGA
BsiEI 10 CGGCCG CG[GA][CT]^CG
2066 CGACCG
BspEI 3322 TCCGGA T^CCGGA
4473 TCCGGA
BspMI 1517 ACCTGC ACCTGC(4/8)
1797 ACCTGC
BsrBI 65 CCGCTC CCGCTC(-3/-3)
1312 GAGCGG
BstAPI 3705 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3714 GCAAGACATGC
Eam1105I 2572 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3733 GACACACGGTC
FbaI 3131 TGATCA T^GATCA
4377 TGATCA
MluNI 1838 TGGCCA TGG^CCA
4557 TGGCCA
MroI 3322 TCCGGA T^CCGGA
4473 TCCGGA
MscI 1838 TGGCCA TGG^CCA
4557 TGGCCA
NcoI 822 CCATGG C^CATGG
4485 CCATGG
SacI 325 GAGCTC GAGCT^C
1927 GAGCTC
SspI 295 AATATT AAT^ATT
4061 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI