##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccII AccIII AgeI AocI
Aor51HI AscI Asp718I AspI AviII
AvrII BbeI BbrPI BcgI BlnI
BsaAI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BssHII BstUI Bsu36I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I Eco81I FseI FspI
HaeII KasI KpnI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SbfI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I Tth111I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 7077 GACGTC GACGT^C
AflIII 2468 ACATGT A^C[GA][CT]GT
ApaI 8277 GGGCCC GGGCC^C
AvaI 5151 CTCGAG C^[CT]CG[GA]G
BalI 5555 TGGCCA TGG^CCA
BclI 8353 TGATCA T^GATCA
BglI 6615 GCCCTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 114 ACCGGA [AT]^CCGG[AT]
BsmBI 5499 CGTCTC CGTCTC(1/5)
BsoBI 5151 CTCGAG C^[CT]CG[GA]G
BspLU11I 2468 ACATGT A^CATGT
BspMI 1505 GCAGGT ACCTGC(4/8)
BssSI 7752 CTCGTG CACGAG(-5/-1)
Bst1107I 9714 GTATAC GTA^TAC
BstAPI 4808 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2982 TTCGAA TT^CGAA
BstEII 8410 GGTTACC G^GT[ACGT]ACC
BstPI 8410 GGTTACC G^GT[ACGT]ACC
BstZ17I 9714 GTATAC GTA^TAC
CfoI 7018 GCGC GCG^C
EcoNI 6779 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 8410 GGTTACC G^GT[ACGT]ACC
FauI 5035 GCGGG CCCGC(4/6)
FbaI 8353 TGATCA T^GATCA
HhaI 7018 GCGC GCG^C
HinP1I 7018 GCGC G^CGC
MfeI 4050 CAATTG C^AATTG
MluNI 5555 TGGCCA TGG^CCA
MscI 5555 TGGCCA TGG^CCA
MunI 4050 CAATTG C^AATTG
NspV 2982 TTCGAA TT^CGAA
PaeR7I 5151 CTCGAG C^TCGAG
PpuMI 7433 GGGACCC [GA]G^G[AT]CC[CT]
SalI 6630 GTCGAC G^TCGAC
ScaI 4352 AGTACT AGT^ACT
SfuI 2982 TTCGAA TT^CGAA
StuI 4221 AGGCCT AGG^CCT
SwaI 3879 ATTTAAAT ATTT^AAAT
XhoI 5151 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AclI 4956 AACGTT AA^CGTT
7484 AACGTT
AcyI 7077 GACGTC G[GA]^CG[CT]C
8469 GGCGTC
Alw44I 1831 GTGCAC G^TGCAC
3282 GTGCAC
ApaLI 1831 GTGCAC G^TGCAC
3282 GTGCAC
BanI 4511 GGCACC G^G[CT][GA]CC
6902 GGTGCC
BcnI 4826 CCGGG CC^[GC]GG
5873 CCCGG
BlpI 740 GCTCAGC GC^T[ACGT]AGC
8819 GCTGAGC
Bpu1102I 740 GCTCAGC GC^T[ACGT]AGC
8819 GCTGAGC
BsaHI 7077 GACGTC G[GA]^CG[CT]C
8469 GGCGTC
BsaI 4311 GAGACC GGTCTC(1/5)
7092 GAGACC
BsrDI 4702 CATTGC GCAATG(2/0)
6156 CATTGC
BstXI 4219 CCAGGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7046 CCAAGAGCATGG
CelII 740 GCTCAGC GC^T[ACGT]AGC
8819 GCTGAGC
DraII 7433 GGGACCC [GA]G^G[ACGT]CC[CT]
8277 GGGCCCT
DrdI 1365 GACACAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8916 GACAAAAAAGTC
EcoO109I 7433 GGGACCC [GA]G^G[ACGT]CC[CT]
8277 GGGCCCT
EcoRI 2209 GAATTC G^AATTC
8608 GAATTC
EcoRV 4868 GATATC GAT^ATC
7924 GATATC
HgaI 5908 GACGC GACGC(5/10)
8470 GCGTC
Hin1I 7077 GACGTC G[GA]^CG[CT]C
8469 GGCGTC
HpaI 9395 GTTAAC GTT^AAC
9732 GTTAAC
NciI 4826 CCGGG CC^[GC]GG
5873 CCCGG
NheI 4817 GCTAGC G^CTAGC
6575 GCTAGC
Psp1406I 4956 AACGTT AA^CGTT
7484 AACGTT
SpeI 6325 ACTAGT A^CTAGT
7532 ACTAGT
SphI 4367 GCATGC GCATG^C
6698 GCATGC
XbaI 2117 TCTAGA T^CTAGA
9028 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AhdI AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspEI AspHI
AvaII BamHI BanII BbsI BbvCI
BbvI BciVI BfaI BfrI BglII
BmyI BpmI BpuAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DraI DsaI
EaeI Eam1105I EarI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PshBI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI