##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AflII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspI
AviII AvrII BcgI BclI BfrI
BlnI BsgI BsiWI BspDI BssHII
Bst1107I BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eco47III EcoO65I
EcoRV EcoT22I FbaI FspI HpaI
KspI MfeI MunI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
PvuI RsrII SacII SalI SapI
SfiI SgrAI SnaBI SpeI SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 948 GGTACC G^GTACC
AccI 1967 GTAGAC GT^[AC][GT]AC
AccIII 453 TCCGGA T^CCGGA
AclI 1520 AACGTT AA^CGTT
AhdI 4354 GACGACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 482 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 948 GGTACC G^GTACC
AspEI 4354 GACGACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2772 CCCGGG C^[CT]CG[GA]G
BbeI 3126 GGCGCC GGCGC^C
BbrPI 4073 CACGTG CAC^GTG
BbvCI 3354 GCTGAGG CCTCAGC(-5/-2)
BciVI 3216 GTATCC GTATCC(6/5)
BglI 4529 GCCTCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1237 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1237 GCTGAGC GC^T[ACGT]AGC
BsaAI 4073 CACGTG [CT]AC^GT[GA]
BsaBI 3905 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 453 TCCGGA [AT]^CCGG[AT]
BseAI 453 TCCGGA T^CCGGA
BsmBI 4417 GAGACG CGTCTC(1/5)
BsoBI 2772 CCCGGG C^[CT]CG[GA]G
Bsp1407I 871 TGTACA T^GTACA
BspEI 453 TCCGGA T^CCGGA
BspLU11I 252 ACATGT A^CATGT
BsrBI 3045 CCGCTC CCGCTC(-3/-3)
BsrDI 3292 CATTGC GCAATG(2/0)
BsrGI 871 TGTACA T^GTACA
BssSI 4395 CACGAG CACGAG(-5/-1)
BstBI 810 TTCGAA TT^CGAA
CelII 1237 GCTGAGC GC^T[ACGT]AGC
DraIII 1123 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4354 GACGACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 148 GGCCGGCC GGCCGG^CC
HincII 3308 GTCAAC GT[CT]^[GA]AC
HindII 3308 GTCAAC GT[CT]^[GA]AC
KasI 3126 GGCGCC G^GCGCC
KpnI 948 GGTACC GGTAC^C
MamI 3905 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 106 ACGCGT A^CGCGT
MroI 453 TCCGGA T^CCGGA
NarI 3126 GGCGCC GG^CGCC
NspV 810 TTCGAA TT^CGAA
PmaCI 4073 CACGTG CAC^GTG
PmlI 4073 CACGTG CAC^GTG
Psp1406I 1520 AACGTT AA^CGTT
SbfI 2407 CCTGCAGG CCTGCA^GG
ScaI 879 AGTACT AGT^ACT
SfoI 3126 GGCGCC GGC^GCC
SfuI 810 TTCGAA TT^CGAA
SmaI 2772 CCCGGG CCC^GGG
SphI 2352 GCATGC GCATG^C
Sse8387I 2407 CCTGCAGG CCTGCA^GG
SspBI 871 TGTACA T^GTACA
XmaCI 2772 CCCGGG C^CCGGG
XmaI 2772 CCCGGG C^CCGGG
XmnI 482 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 106 ACGCGT A^C[GA][CT]GT
252 ACATGT
AlwNI 1772 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
2805 CAGCAGCTG
AocI 4288 CCTGAGG CC^T[ACGT]AGG
4312 CCTCAGG
ApaI 353 GGGCCC GGGCC^C
4100 GGGCCC
BamHI 1131 GGATCC G^GATCC
3329 GGATCC
BglII 972 AGATCT A^GATCT
1474 AGATCT
BsiEI 151 CGGCCG CG[GA][CT]^CG
3211 CGGCCG
BsmI 4377 GAATGC GAATGC(1/-1)
2250 GCATTC
BspHI 237 TCATGA T^CATGA
888 TCATGA
BstAPI 2352 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2804 GCAGCAGCTGC
Bsu36I 4288 CCTGAGG CC^T[ACGT]AGG
4312 CCTCAGG
EagI 151 CGGCCG C^GGCCG
3211 CGGCCG
EclXI 151 CGGCCG C^GGCCG
3211 CGGCCG
Eco52I 151 CGGCCG C^GGCCG
3211 CGGCCG
Eco81I 4288 CCTGAGG CC^T[ACGT]AGG
4312 CCTCAGG
HaeII 2240 GGCGCT [GA]GCGC^[CT]
3126 GGCGCC
HindIII 692 AAGCTT A^AGCTT
2222 AAGCTT
NcoI 1363 CCATGG C^CATGG
2705 CCATGG
PvuII 2808 CAGCTG CAG^CTG
3496 CAGCTG
RcaI 237 TCATGA T^CATGA
888 TCATGA
SexAI 1065 ACCAGGT A^CC[AT]GGT
1356 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII