##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AhdI AscI AseI AsnI
Asp700I AspEI BbeI BbrPI BcgI
BclI BfrI BsaAI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoRI FbaI FseI
HpaI KasI KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SspBI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 3811 GGTACC G^GTACC
AcyI 11 GACGCC G[GA]^CG[CT]C
AgeI 3664 ACCGGT A^CCGGT
AocI 47 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3817 AGCGCT AGC^GCT
Asp718I 3811 GGTACC G^GTACC
AspI 199 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1757 TGCGCA TGC^GCA
AvrII 3747 CCTAGG C^CTAGG
BamHI 1364 GGATCC G^GATCC
BciVI 812 GTATCC GTATCC(6/5)
BglII 1025 AGATCT A^GATCT
BlnI 3747 CCTAGG C^CTAGG
BsaHI 11 GACGCC G[GA]^CG[CT]C
BsaWI 3664 ACCGGT [AT]^CCGG[AT]
BsmI 1788 GCATTC GAATGC(1/-1)
BsrDI 3680 GCAATG GCAATG(2/0)
BssSI 1918 CACGAG CACGAG(-5/-1)
BstAPI 328 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 450 GGTAACC G^GT[ACGT]ACC
BstPI 450 GGTAACC G^GT[ACGT]ACC
Bsu36I 47 CCTCAGG CC^T[ACGT]AGG
DraI 399 TTTAAA TTT^AAA
Eco47III 3817 AGCGCT AGC^GCT
Eco81I 47 CCTCAGG CC^T[ACGT]AGG
EcoO65I 450 GGTAACC G^GT[ACGT]ACC
EcoRV 2131 GATATC GAT^ATC
EcoT22I 214 ATGCAT ATGCA^T
FauI 1086 GCGGG CCCGC(4/6)
FspI 1757 TGCGCA TGC^GCA
HaeII 3817 AGCGCT [GA]GCGC^[CT]
Hin1I 11 GACGCC G[GA]^CG[CT]C
HindIII 692 AAGCTT A^AGCTT
KpnI 3811 GGTACC GGTAC^C
NsiI 214 ATGCAT ATGCA^T
PflFI 199 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3664 ACCGGT A^CCGGT
SacI 3533 GAGCTC GAGCT^C
SapI 2903 GCTCTTC GCTCTTC(1/4)
ScaI 3037 AGTACT AGT^ACT
Tth111I 199 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 1394 CGCG CG^CG
1999 CGCG
AcsI 1011 AAATTC [GA]^AATT[CT]
4601 AAATTT
ApoI 1011 AAATTC [GA]^AATT[CT]
4601 AAATTT
BglI 1120 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1729 GCCAGTGAGGC
BlpI 481 GCTCAGC GC^T[ACGT]AGC
2727 GCTTAGC
Bpu1102I 481 GCTCAGC GC^T[ACGT]AGC
2727 GCTTAGC
BsaBI 1252 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1467 GATCCCCATC
BseRI 1684 CTCCTC GAGGAG(10/8)
2255 GAGGAG
BsmFI 346 GTCCC GGGAC(10/14)
1864 GTCCC
BsrFI 272 ACCGGC [GA]^CCGG[CT]
3664 ACCGGT
BstUI 1394 CGCG CG^CG
1999 CGCG
BstXI 74 CCAGAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
555 CCATGAGGGTGG
CelII 481 GCTCAGC GC^T[ACGT]AGC
2727 GCTTAGC
Cfr10I 272 ACCGGC [GA]^CCGG[CT]
3664 ACCGGT
HincII 268 GTCAAC GT[CT]^[GA]AC
2242 GTCAAC
HindII 268 GTCAAC GT[CT]^[GA]AC
2242 GTCAAC
MaeII 1202 ACGT A^CGT
2240 ACGT
MamI 1252 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1467 GATCCCCATC
MfeI 635 CAATTG C^AATTG
869 CAATTG
MunI 635 CAATTG C^AATTG
869 CAATTG
MvnI 1394 CGCG CG^CG
1999 CGCG
SmaI 141 CCCGGG CCC^GGG
1295 CCCGGG
SmlI 1213 CTTGAG C^T[CT][GA]AG
1942 CTCAAG
SpeI 2488 ACTAGT A^CTAGT
3934 ACTAGT
SphI 1951 GCATGC GCATG^C
2525 GCATGC
SspI 622 AATATT AAT^ATT
1520 AATATT
XmaCI 141 CCCGGG C^CCGGG
1295 CCCGGG
XmaI 141 CCCGGG C^CCGGG
1295 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI ApaI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII