##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccI AclI AcsI AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BciVI BclI BglII
BlnI BsiWI BsmI BspDI BspHI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoRI EcoRV EcoT22I FbaI FspI
HindIII HpaI KpnI MluI NdeI
NheI NotI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SfuI SnaBI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1899 GACGTC GACGT^C
AccIII 2552 TCCGGA T^CCGGA
AflII 1320 CTTAAG C^TTAAG
AflIII 1328 ACATGT A^C[GA][CT]GT
Asp700I 1431 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3857 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1325 GCAACATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1320 CTTAAG C^TTAAG
BsaBI 1177 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2552 TCCGGA [AT]^CCGG[AT]
BseAI 2552 TCCGGA T^CCGGA
Bsp1407I 1923 TGTACA T^GTACA
BspEI 2552 TCCGGA T^CCGGA
BspLU11I 1328 ACATGT A^CATGT
BsrGI 1923 TGTACA T^GTACA
BssHII 371 GCGCGC G^CGCGC
DraIII 2543 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 27 CGGCCG C^GGCCG
EclXI 27 CGGCCG C^GGCCG
Eco52I 27 CGGCCG C^GGCCG
EcoNI 1942 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 24 GGCCGGCC GGCCGG^CC
HincII 685 GTCAAC GT[CT]^[GA]AC
HindII 685 GTCAAC GT[CT]^[GA]AC
MamI 1177 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1116 CAATTG C^AATTG
MroI 2552 TCCGGA T^CCGGA
MunI 1116 CAATTG C^AATTG
NcoI 3683 CCATGG C^CATGG
NruI 230 TCGCGA TCG^CGA
PflFI 3857 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SgrAI 491 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 3263 CCCGGG CCC^GGG
SpeI 304 ACTAGT A^CTAGT
SspBI 1923 TGTACA T^GTACA
Tsp509I 1117 AATT ^AATT
Tth111I 3857 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3263 CCCGGG C^CCGGG
XmaI 3263 CCCGGG C^CCGGG
XmnI 1431 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 795 GTAC GT^AC
1924 GTAC
BbrPI 1636 CACGTG CAC^GTG
3613 CACGTG
BfaI 305 CTAG C^TAG
3425 CTAG
BlpI 1536 GCTCAGC GC^T[ACGT]AGC
1797 GCTGAGC
Bpu1102I 1536 GCTCAGC GC^T[ACGT]AGC
1797 GCTGAGC
BsaAI 1636 CACGTG [CT]AC^GT[GA]
3613 CACGTG
BsmBI 1303 GAGACG CGTCTC(1/5)
1831 GAGACG
BsrDI 3300 CATTGC GCAATG(2/0)
733 GCAATG
BssSI 1690 CACGAG CACGAG(-5/-1)
982 CTCGTG
BstEII 747 GGTCACC G^GT[ACGT]ACC
1960 GGTCACC
BstPI 747 GGTCACC G^GT[ACGT]ACC
1960 GGTCACC
BstXI 2538 CCAGCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3347 CCAGGAGGCTGG
CelII 1536 GCTCAGC GC^T[ACGT]AGC
1797 GCTGAGC
EcoO65I 747 GGTCACC G^GT[ACGT]ACC
1960 GGTCACC
MaeI 305 CTAG C^TAG
3425 CTAG
MseI 71 TTAA T^TAA
1321 TTAA
MslI 1077 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3609 CACCCACGTG
NaeI 25 GCCGGC GCC^GGC
492 GCCGGC
NgoMIV 25 GCCGGC G^CCGGC
492 GCCGGC
PmaCI 1636 CACGTG CAC^GTG
3613 CACGTG
PmlI 1636 CACGTG CAC^GTG
3613 CACGTG
RsaI 795 GTAC GT^AC
1924 GTAC
SacI 1465 GAGCTC GAGCT^C
1693 GAGCTC
SapI 2764 GAAGAGC GCTCTTC(1/4)
3099 GAAGAGC
SexAI 2126 ACCTGGT A^CC[AT]GGT
2544 ACCTGGT
SfiI 1473 GGCCCAAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2517 GGCCAGGGGGGCC
SphI 1601 GCATGC GCATG^C
1764 GCATGC
TaqI 1171 TCGA T^CGA
1334 TCGA
TfiI 816 GATTC G^A[AT]TC
1182 GAATC
Tru9I 71 TTAA T^TAA
1321 TTAA
TthHB8I 1171 TCGA T^CGA
1334 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TseI Tsp45I TspRI
Van91I XcmI XhoII