##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AgeI AhdI
AocI Aor51HI AscI Asp718I AspEI
AspI AvrII BbeI BbrPI BbvCI
BcgI BciVI BcnI BglI BlnI
BsiEI BsiWI BspDI BsrFI BssHII
BstBI BstEII BstPI BstUI Bsu36I
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FseI HaeII KasI
KpnI KspI MluI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 1621 TCCGGA T^CCGGA
AclI 331 AACGTT AA^CGTT
AcyI 3044 GGCGTC G[GA]^CG[CT]C
AflII 2028 CTTAAG C^TTAAG
Alw44I 3520 GTGCAC G^TGCAC
ApaLI 3520 GTGCAC G^TGCAC
AvaI 611 CTCGGG C^[CT]CG[GA]G
AvaII 2815 GGACC G^G[AT]CC
AviII 4450 TGCGCA TGC^GCA
BalI 1549 TGGCCA TGG^CCA
BclI 2581 TGATCA T^GATCA
BfrI 2028 CTTAAG C^TTAAG
BglII 104 AGATCT A^GATCT
BsaAI 4332 TACGTG [CT]AC^GT[GA]
BsaHI 3044 GGCGTC G[GA]^CG[CT]C
BsaI 3175 GAGACC GGTCTC(1/5)
BseAI 1621 TCCGGA T^CCGGA
BsmBI 855 GAGACG CGTCTC(1/5)
BsoBI 611 CTCGGG C^[CT]CG[GA]G
BspEI 1621 TCCGGA T^CCGGA
BsrBI 3456 GAGCGG CCGCTC(-3/-3)
Bst1107I 4306 GTATAC GTA^TAC
BstZ17I 4306 GTATAC GTA^TAC
CfoI 4451 GCGC GCG^C
DraIII 2551 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1063 GACAAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 463 CCATGG C^C[GA][CT]GG
EaeI 1549 TGGCCA [CT]^GGCC[GA]
EcoRI 3013 GAATTC G^AATTC
FauI 3458 GCGGG CCCGC(4/6)
FbaI 2581 TGATCA T^GATCA
FspI 4450 TGCGCA TGC^GCA
HgaI 3045 GCGTC GACGC(5/10)
HhaI 4451 GCGC GCG^C
Hin1I 3044 GGCGTC G[GA]^CG[CT]C
HinP1I 4451 GCGC G^CGC
HincII 32 GTTAAC GT[CT]^[GA]AC
HindII 32 GTTAAC GT[CT]^[GA]AC
HindIII 3610 AAGCTT A^AGCTT
HpaI 32 GTTAAC GTT^AAC
MluNI 1549 TGGCCA TGG^CCA
MroI 1621 TCCGGA T^CCGGA
MscI 1549 TGGCCA TGG^CCA
NcoI 463 CCATGG C^CATGG
NdeI 2577 CATATG CA^TATG
PflMI 2510 CCAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2816 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 331 AACGTT AA^CGTT
PvuII 3555 CAGCTG CAG^CTG
SexAI 1933 ACCTGGT A^CC[AT]GGT
SpeI 1497 ACTAGT A^CTAGT
StuI 2400 AGGCCT AGG^CCT
SwaI 4802 ATTTAAAT ATTT^AAAT
Van91I 2510 CCAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2526 GTAGAC GT^[AC][GT]AC
4306 GTATAC
ApaI 2726 GGGCCC GGGCC^C
4086 GGGCCC
AseI 1974 ATTAAT AT^TAAT
2676 ATTAAT
AsnI 1974 ATTAAT AT^TAAT
2676 ATTAAT
BamHI 2489 GGATCC G^GATCC
3629 GGATCC
BanI 3917 GGTGCC G^G[CT][GA]CC
4882 GGTGCC
BanII 2726 GGGCCC G[GA]GC[CT]^C
4086 GGGCCC
BsaBI 564 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
837 GATATAGATC
BsaWI 1558 ACCGGA [AT]^CCGG[AT]
1621 TCCGGA
BsmI 674 GAATGC GAATGC(1/-1)
896 GAATGC
BspHI 1025 TCATGA T^CATGA
2884 TCATGA
BssSI 253 CACGAG CACGAG(-5/-1)
3733 CTCGTG
BstXI 3025 CCAATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3286 CCAAAATAATGG
HapII 1559 CCGG C^CGG
1622 CCGG
HpaII 1559 CCGG C^CGG
1622 CCGG
MamI 564 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
837 GATATAGATC
MfeI 3211 CAATTG C^AATTG
3220 CAATTG
MspA1I 3555 CAGCTG C[AC]G^C[GT]G
4155 CAGCGG
MspI 1559 CCGG C^CGG
1622 CCGG
MunI 3211 CAATTG C^AATTG
3220 CAATTG
PshBI 1974 ATTAAT AT^TAAT
2676 ATTAAT
RcaI 1025 TCATGA T^CATGA
2884 TCATGA
SphI 2168 GCATGC GCATG^C
2980 GCATGC
XbaI 1059 TCTAGA T^CTAGA
2054 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
BbsI BbvI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspLU11I BspMI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CelII
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI