##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AclI AflII AocI AscI
AspI AvrII BfrI BlnI BsiWI
BsmI Bsp1407I BspDI BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI Eco81I EcoRI EcoRV
FseI HindIII HpaI KspI MluI
NdeI NheI NotI NruI NspV
PacI PflFI PmeI PshAI Psp1406I
PvuI RsrII SacII SbfI SexAI
SfiI SfuI SgrAI SnaBI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 5293 GGTACC G^GTACC
AccI 1319 GTCGAC GT^[AC][GT]AC
AccIII 5013 TCCGGA T^CCGGA
AcsI 1066 GAATTT [GA]^AATT[CT]
AgeI 2733 ACCGGT A^CCGGT
ApoI 1066 GAATTT [GA]^AATT[CT]
AseI 5460 ATTAAT AT^TAAT
AsnI 5460 ATTAAT AT^TAAT
Asp700I 2662 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5293 GGTACC G^GTACC
AviII 3441 TGCGCA TGC^GCA
BbrPI 2192 CACGTG CAC^GTG
BclI 2526 TGATCA T^GATCA
BsaBI 604 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 5013 TCCGGA T^CCGGA
BspEI 5013 TCCGGA T^CCGGA
BstEII 3175 GGTCACC G^GT[ACGT]ACC
BstPI 3175 GGTCACC G^GT[ACGT]ACC
DraIII 5187 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3175 GGTCACC G^GT[ACGT]ACC
EcoT22I 1004 ATGCAT ATGCA^T
FbaI 2526 TGATCA T^GATCA
FspI 3441 TGCGCA TGC^GCA
KpnI 5293 GGTACC GGTAC^C
MamI 604 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3706 CAATTG C^AATTG
MroI 5013 TCCGGA T^CCGGA
MunI 3706 CAATTG C^AATTG
NsiI 1004 ATGCAT ATGCA^T
PaeR7I 2099 CTCGAG C^TCGAG
PinAI 2733 ACCGGT A^CCGGT
PmaCI 2192 CACGTG CAC^GTG
PmlI 2192 CACGTG CAC^GTG
PshBI 5460 ATTAAT AT^TAAT
SalI 1319 GTCGAC G^TCGAC
ScaI 582 AGTACT AGT^ACT
SmlI 2099 CTCGAG C^T[CT][GA]AG
SpeI 172 ACTAGT A^CTAGT
XhoI 2099 CTCGAG C^TCGAG
XmnI 2662 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 2943 ACATGT A^C[GA][CT]GT
4176 ACATGT
AhdI 2108 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3746 GACCAGGAGTC
Aor51HI 1646 AGCGCT AGC^GCT
2264 AGCGCT
AspEI 2108 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3746 GACCAGGAGTC
BamHI 2070 GGATCC G^GATCC
5044 GGATCC
BcgI 630 GCATGGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2223 GCATCACCATCG
BciVI 2686 GGATAC GTATCC(6/5)
121 GTATCC
BlpI 3905 GCTGAGC GC^T[ACGT]AGC
4681 GCTGAGC
Bpu1102I 3905 GCTGAGC GC^T[ACGT]AGC
4681 GCTGAGC
BsaAI 1211 TACGTG [CT]AC^GT[GA]
2192 CACGTG
BsmBI 152 GAGACG CGTCTC(1/5)
2561 GAGACG
BspLU11I 2943 ACATGT A^CATGT
4176 ACATGT
BssSI 647 CACGAG CACGAG(-5/-1)
1595 CACGAG
CelII 3905 GCTGAGC GC^T[ACGT]AGC
4681 GCTGAGC
EagI 1482 CGGCCG C^GGCCG
1693 CGGCCG
Eam1105I 2108 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3746 GACCAGGAGTC
EclXI 1482 CGGCCG C^GGCCG
1693 CGGCCG
Eco47III 1646 AGCGCT AGC^GCT
2264 AGCGCT
Eco52I 1482 CGGCCG C^GGCCG
1693 CGGCCG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI