##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AflII AhdI Aor51HI ApaI
AscI AseI AsnI AspEI AviII
BbeI BbvCI BcgI BfrI BglII
BseAI BsiEI BsiWI BspDI BspEI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FseI FspI HpaI
KasI KspI MluI MroI NarI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshBI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1336 GACGTC GACGT^C
AccII 1500 CGCG CG^CG
AclI 4898 AACGTT AA^CGTT
AgeI 1188 ACCGGT A^CCGGT
AspI 2560 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglI 1650 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1188 ACCGGT [AT]^CCGG[AT]
BsmBI 4662 GAGACG CGTCTC(1/5)
Bsp1407I 694 TGTACA T^GTACA
BsrDI 1342 CATTGC GCAATG(2/0)
BsrGI 694 TGTACA T^GTACA
BssSI 5351 CTCGTG CACGAG(-5/-1)
BstEII 3025 GGTCACC G^GT[ACGT]ACC
BstPI 3025 GGTCACC G^GT[ACGT]ACC
BstUI 1500 CGCG CG^CG
EcoO65I 3025 GGTCACC G^GT[ACGT]ACC
HaeII 1359 AGCGCC [GA]GCGC^[CT]
HincII 296 GTCAAC GT[CT]^[GA]AC
HindII 296 GTCAAC GT[CT]^[GA]AC
MvnI 1500 CGCG CG^CG
NaeI 1791 GCCGGC GCC^GGC
NdeI 5611 CATATG CA^TATG
NgoMIV 1791 GCCGGC G^CCGGC
NheI 4246 GCTAGC G^CTAGC
PflFI 2560 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1188 ACCGGT A^CCGGT
Psp1406I 4898 AACGTT AA^CGTT
SacI 238 GAGCTC GAGCT^C
SphI 1350 GCATGC GCATG^C
SspBI 694 TGTACA T^GTACA
SwaI 5759 ATTTAAAT ATTT^AAAT
Tth111I 2560 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 3835 GGTACC G^GTACC
5385 GGTACC
AccI 2295 GTAGAC GT^[AC][GT]AC
2351 GTCTAC
AcyI 1336 GACGTC G[GA]^CG[CT]C
4664 GACGCC
Alw44I 3215 GTGCAC G^TGCAC
4350 GTGCAC
AocI 2166 CCTGAGG CC^T[ACGT]AGG
5407 CCTCAGG
ApaLI 3215 GTGCAC G^TGCAC
4350 GTGCAC
Asp718I 3835 GGTACC G^GTACC
5385 GGTACC
AvaI 164 CCCGAG C^[CT]CG[GA]G
2803 CTCGGG
AvrII 947 CCTAGG C^CTAGG
5451 CCTAGG
BamHI 159 GGATCC G^GATCC
1853 GGATCC
BanII 238 GAGCTC G[GA]GC[CT]^C
1823 GGGCTC
BbrPI 1256 CACGTG CAC^GTG
4527 CACGTG
BciVI 4674 GGATAC GTATCC(6/5)
4367 GTATCC
BlnI 947 CCTAGG C^CTAGG
5451 CCTAGG
BsaBI 484 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3658 GATTGGCATC
BsaHI 1336 GACGTC G[GA]^CG[CT]C
4664 GACGCC
BsoBI 164 CCCGAG C^[CT]CG[GA]G
2803 CTCGGG
BspHI 1119 TCATGA T^CATGA
5590 TCATGA
BsrBI 2096 CCGCTC CCGCTC(-3/-3)
3397 GAGCGG
BsrFI 1188 ACCGGT [GA]^CCGG[CT]
1791 GCCGGC
Bsu36I 2166 CCTGAGG CC^T[ACGT]AGG
5407 CCTCAGG
Cfr10I 1188 ACCGGT [GA]^CCGG[CT]
1791 GCCGGC
DrdI 953 GACTACCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2931 GACAGGACAGTC
Eco81I 2166 CCTGAGG CC^T[ACGT]AGG
5407 CCTCAGG
EcoRI 4147 GAATTC G^AATTC
4709 GAATTC
Hin1I 1336 GACGTC G[GA]^CG[CT]C
4664 GACGCC
HindIII 981 AAGCTT A^AGCTT
5502 AAGCTT
KpnI 3835 GGTACC GGTAC^C
5385 GGTACC
MamI 484 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3658 GATTGGCATC
MfeI 664 CAATTG C^AATTG
4281 CAATTG
MunI 664 CAATTG C^AATTG
4281 CAATTG
PmaCI 1256 CACGTG CAC^GTG
4527 CACGTG
PmlI 1256 CACGTG CAC^GTG
4527 CACGTG
PshAI 1096 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3149 GACAGTGGTC
RcaI 1119 TCATGA T^CATGA
5590 TCATGA
ScaI 1427 AGTACT AGT^ACT
1878 AGTACT
SexAI 795 ACCAGGT A^CC[AT]GGT
4467 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BbsI BbvI
BclI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoRV
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI