##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI BcgI BclI BfrI
BglII BsiWI BspDI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
DsaI EcoRI EcoRV EcoT22I FbaI
FseI KspI MluI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1358 GGTACC G^GTACC
AccIII 618 TCCGGA T^CCGGA
AflIII 5542 ACATGT A^C[GA][CT]GT
Alw44I 4292 GTGCAC G^TGCAC
Aor51HI 2816 AGCGCT AGC^GCT
ApaI 4203 GGGCCC GGGCC^C
ApaLI 4292 GTGCAC G^TGCAC
Asp718I 1358 GGTACC G^GTACC
AvrII 4874 CCTAGG C^CTAGG
BbeI 100 GGCGCC GGCGC^C
BlnI 4874 CCTAGG C^CTAGG
BlpI 411 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 411 GCTGAGC GC^T[ACGT]AGC
BsaBI 1056 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 618 TCCGGA T^CCGGA
BsiEI 225 CGGCCG CG[GA][CT]^CG
Bsp1407I 3909 TGTACA T^GTACA
BspEI 618 TCCGGA T^CCGGA
BspHI 3646 TCATGA T^CATGA
BspLU11I 5542 ACATGT A^CATGT
BsrBI 286 CCGCTC CCGCTC(-3/-3)
BsrGI 3909 TGTACA T^GTACA
BstEII 4963 GGTGACC G^GT[ACGT]ACC
BstPI 4963 GGTGACC G^GT[ACGT]ACC
CelII 411 GCTGAGC GC^T[ACGT]AGC
EagI 225 CGGCCG C^GGCCG
EclXI 225 CGGCCG C^GGCCG
Eco47III 2816 AGCGCT AGC^GCT
Eco52I 225 CGGCCG C^GGCCG
EcoO65I 4963 GGTGACC G^GT[ACGT]ACC
HincII 1913 GTTAAC GT[CT]^[GA]AC
HindII 1913 GTTAAC GT[CT]^[GA]AC
HindIII 4830 AAGCTT A^AGCTT
HpaI 1913 GTTAAC GTT^AAC
KasI 100 GGCGCC G^GCGCC
KpnI 1358 GGTACC GGTAC^C
MamI 1056 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2982 CAATTG C^AATTG
MroI 618 TCCGGA T^CCGGA
MunI 2982 CAATTG C^AATTG
NaeI 78 GCCGGC GCC^GGC
NarI 100 GGCGCC GG^CGCC
NgoMIV 78 GCCGGC G^CCGGC
PshAI 981 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3646 TCATGA T^CATGA
SapI 4133 GAAGAGC GCTCTTC(1/4)
ScaI 2047 AGTACT AGT^ACT
SexAI 3268 ACCTGGT A^CC[AT]GGT
SfiI 4195 GGCCATGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 100 GGCGCC GGC^GCC
SspBI 3909 TGTACA T^GTACA
SwaI 5264 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 1077 GAATTT [GA]^AATT[CT]
3963 GAATTT
AcyI 100 GGCGCC G[GA]^CG[CT]C
981 GACGCC
AhdI 1900 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4731 GACCTCCAGTC
AocI 1183 CCTGAGG CC^T[ACGT]AGG
1707 CCTCAGG
ApoI 1077 GAATTT [GA]^AATT[CT]
3963 GAATTT
Asp700I 1290 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1832 GAAATACTTC
AspEI 1900 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4731 GACCTCCAGTC
AviII 536 TGCGCA TGC^GCA
665 TGCGCA
BalI 3923 TGGCCA TGG^CCA
4066 TGGCCA
BbrPI 2283 CACGTG CAC^GTG
5081 CACGTG
BciVI 1922 GTATCC GTATCC(6/5)
3242 GTATCC
BsaAI 2283 CACGTG [CT]AC^GT[GA]
5081 CACGTG
BsaHI 100 GGCGCC G[GA]^CG[CT]C
981 GACGCC
BsaWI 618 TCCGGA [AT]^CCGG[AT]
692 TCCGGT
BsmBI 3694 CGTCTC CGTCTC(1/5)
5352 GAGACG
BsmI 3105 GCATTC GAATGC(1/-1)
4246 GCATTC
BssHII 233 GCGCGC G^CGCGC
3733 GCGCGC
Bsu36I 1183 CCTGAGG CC^T[ACGT]AGG
1707 CCTCAGG
DraI 2692 TTTAAA TTT^AAA
5265 TTTAAA
Eam1105I 1900 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4731 GACCTCCAGTC
Eco81I 1183 CCTGAGG CC^T[ACGT]AGG
1707 CCTCAGG
FspI 536 TGCGCA TGC^GCA
665 TGCGCA
Hin1I 100 GGCGCC G[GA]^CG[CT]C
981 GACGCC
MluNI 3923 TGGCCA TGG^CCA
4066 TGGCCA
MscI 3923 TGGCCA TGG^CCA
4066 TGGCCA
NheI 2584 GCTAGC G^CTAGC
3226 GCTAGC
NspI 5472 GCATGT [GA]CATG^[CT]
5542 ACATGT
PmaCI 2283 CACGTG CAC^GTG
5081 CACGTG
PmlI 2283 CACGTG CAC^GTG
5081 CACGTG
SmaI 3788 CCCGGG CCC^GGG
4662 CCCGGG
StuI 329 AGGCCT AGG^CCT
1292 AGGCCT
XmaCI 3788 CCCGGG C^CCGGG
4662 CCCGGG
XmaI 3788 CCCGGG C^CCGGG
4662 CCCGGG
XmnI 1290 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1832 GAAATACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AspHI AspI AvaI
AvaII BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII