##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccII AgeI AhdI Aor51HI AscI
AspEI AviII AvrII BbvCI BcgI
BclI BcnI BglI BlnI BsiEI
BsiWI BspDI BsrFI BssHII BstEII
BstPI BstUI Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FbaI
FseI FspI HpaI KspI MluI
MvnI NaeI NciI NgoMIV NotI
NruI PacI PinAI PmeI PvuI
RsrII SacII SbfI SfiI SgrAI
SmaI SnaBI Sse8387I SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2463 GACGTC GACGT^C
Acc65I 3874 GGTACC G^GTACC
AclI 550 AACGTT AA^CGTT
Alw44I 1689 GTGCAC G^TGCAC
ApaI 3249 GGGCCC GGGCC^C
ApaLI 1689 GTGCAC G^TGCAC
AseI 6950 ATTAAT AT^TAAT
AsnI 6950 ATTAAT AT^TAAT
Asp718I 3874 GGTACC G^GTACC
BamHI 1634 GGATCC G^GATCC
BciVI 2183 GTATCC GTATCC(6/5)
BlpI 3692 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3692 GCTGAGC GC^T[ACGT]AGC
BsaBI 3752 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2099 GGTCTC GGTCTC(1/5)
BsrBI 3829 CCGCTC CCGCTC(-3/-3)
Bst1107I 2215 GTATAC GTA^TAC
BstZ17I 2215 GTATAC GTA^TAC
CelII 3692 GCTGAGC GC^T[ACGT]AGC
DsaI 3114 CCATGG C^C[GA][CT]GG
FauI 3828 CCCGC CCCGC(4/6)
KpnI 3874 GGTACC GGTAC^C
MamI 3752 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 3114 CCATGG C^CATGG
NdeI 1398 CATATG CA^TATG
PflMI 451 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 6950 ATTAAT AT^TAAT
Psp1406I 550 AACGTT AA^CGTT
SalI 1548 GTCGAC G^TCGAC
SapI 3201 GCTCTTC GCTCTTC(1/4)
ScaI 3680 AGTACT AGT^ACT
SphI 1224 GCATGC GCATG^C
StuI 2521 AGGCCT AGG^CCT
Van91I 451 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4051 TCTAGA T^CTAGA
XcmI 3678 CCAGTACTCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 214 TCCGGA T^CCGGA
805 TCCGGA
AflII 6422 CTTAAG C^TTAAG
7120 CTTAAG
AocI 4625 CCTCAGG CC^T[ACGT]AGG
6235 CCTGAGG
AspI 3499 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4017 GACCTGGTC
BbeI 4943 GGCGCC GGCGC^C
5784 GGCGCC
BbrPI 5082 CACGTG CAC^GTG
6216 CACGTG
BfrI 6422 CTTAAG C^TTAAG
7120 CTTAAG
BsaAI 5082 CACGTG [CT]AC^GT[GA]
6216 CACGTG
BsaWI 214 TCCGGA [AT]^CCGG[AT]
805 TCCGGA
BseAI 214 TCCGGA T^CCGGA
805 TCCGGA
BspEI 214 TCCGGA T^CCGGA
805 TCCGGA
BspHI 5605 TCATGA T^CATGA
6453 TCATGA
Bsu36I 4625 CCTCAGG CC^T[ACGT]AGG
6235 CCTGAGG
DraIII 1692 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
5544 CACGAGGTG
Eco81I 4625 CCTCAGG CC^T[ACGT]AGG
6235 CCTGAGG
EcoT22I 5437 ATGCAT ATGCA^T
6035 ATGCAT
KasI 4943 GGCGCC G^GCGCC
5784 GGCGCC
MroI 214 TCCGGA T^CCGGA
805 TCCGGA
NarI 4943 GGCGCC GG^CGCC
5784 GGCGCC
NheI 517 GCTAGC G^CTAGC
6469 GCTAGC
NsiI 5437 ATGCAT ATGCA^T
6035 ATGCAT
PflFI 3499 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4017 GACCTGGTC
PmaCI 5082 CACGTG CAC^GTG
6216 CACGTG
PmlI 5082 CACGTG CAC^GTG
6216 CACGTG
PshAI 2231 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3623 GACACAGGTC
PvuII 1953 CAGCTG CAG^CTG
5946 CAGCTG
RcaI 5605 TCATGA T^CATGA
6453 TCATGA
SfoI 4943 GGCGCC GGC^GCC
5784 GGCGCC
Tth111I 3499 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4017 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BfaI
BglII BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrDI BsrGI BsrI BssKI BssSI
BstAPI BstBI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NdeII NlaIII
NlaIV NspI NspV PaeR7I PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfuI SmlI SpeI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoI
XhoII XmnI