##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AhdI Aor51HI AscI
AseI AsnI Asp700I AspEI AviII
BalI BsiWI BsmI BspDI BspHI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraIII DrdI Eam1105I Eco47III
EcoRV EcoT22I FseI FspI HindIII
HpaI MfeI MluI MluNI MscI
MunI NaeI NdeI NgoMIV NheI
NruI NsiI NspV PacI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfuI SgrAI SnaBI Sse8387I XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 5821 GGTACC G^GTACC
AccIII 2544 TCCGGA T^CCGGA
AgeI 803 ACCGGT A^CCGGT
Asp718I 5821 GGTACC G^GTACC
AspI 599 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 282 CCTAGG C^CTAGG
BamHI 476 GGATCC G^GATCC
BbeI 683 GGCGCC GGCGC^C
BciVI 2448 GGATAC GTATCC(6/5)
BglII 2638 AGATCT A^GATCT
BlnI 282 CCTAGG C^CTAGG
BlpI 138 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 138 GCTCAGC GC^T[ACGT]AGC
BseAI 2544 TCCGGA T^CCGGA
BsmBI 34 GAGACG CGTCTC(1/5)
BspEI 2544 TCCGGA T^CCGGA
BstEII 948 GGTGACC G^GT[ACGT]ACC
BstPI 948 GGTGACC G^GT[ACGT]ACC
CelII 138 GCTCAGC GC^T[ACGT]AGC
EcoO65I 948 GGTGACC G^GT[ACGT]ACC
EcoRI 609 GAATTC G^AATTC
KasI 683 GGCGCC G^GCGCC
KpnI 5821 GGTACC GGTAC^C
MroI 2544 TCCGGA T^CCGGA
NarI 683 GGCGCC GG^CGCC
NotI 1 GCGGCCGC GC^GGCCGC
PflFI 599 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1140 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 803 ACCGGT A^CCGGT
SapI 1386 GCTCTTC GCTCTTC(1/4)
SfiI 103 GGCCTCACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 683 GGCGCC GGC^GCC
SpeI 6013 ACTAGT A^CTAGT
SphI 5300 GCATGC GCATG^C
StuI 3138 AGGCCT AGG^CCT
SwaI 3015 ATTTAAAT ATTT^AAAT
Tth111I 599 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1140 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 4972 CTTAAG C^TTAAG
6330 CTTAAG
BanI 683 GGCGCC G^G[CT][GA]CC
5821 GGTACC
BbrPI 929 CACGTG CAC^GTG
1405 CACGTG
BcgI 2885 CGAGTCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5963 CGAAGTTCGTGC
BclI 360 TGATCA T^GATCA
4565 TGATCA
BfrI 4972 CTTAAG C^TTAAG
6330 CTTAAG
BsaBI 438 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4211 GATTGTCATC
BsaWI 803 ACCGGT [AT]^CCGG[AT]
2544 TCCGGA
BsiEI 2 CGGCCG CG[GA][CT]^CG
972 CGGCCG
BspLU11I 2908 ACATGT A^CATGT
4701 ACATGT
BssSI 1978 CACGAG CACGAG(-5/-1)
3255 CTCGTG
BstAPI 2246 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5234 GCAACCTCTGC
EagI 2 CGGCCG C^GGCCG
972 CGGCCG
EclXI 2 CGGCCG C^GGCCG
972 CGGCCG
Eco52I 2 CGGCCG C^GGCCG
972 CGGCCG
FbaI 360 TGATCA T^GATCA
4565 TGATCA
HincII 448 GTCAAC GT[CT]^[GA]AC
2811 GTCAAC
HindII 448 GTCAAC GT[CT]^[GA]AC
2811 GTCAAC
KspI 5 CCGCGG CCGC^GG
2229 CCGCGG
MamI 438 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4211 GATTGTCATC
NcoI 1058 CCATGG C^CATGG
5646 CCATGG
PaeR7I 467 CTCGAG C^TCGAG
1924 CTCGAG
PmaCI 929 CACGTG CAC^GTG
1405 CACGTG
PmlI 929 CACGTG CAC^GTG
1405 CACGTG
SacII 5 CCGCGG CCGC^GG
2229 CCGCGG
XbaI 639 TCTAGA T^CTAGA
5778 TCTAGA
XhoI 467 CTCGAG C^TCGAG
1924 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AspHI AvaI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI