##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccIII AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AspI AvaI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BglI BglII BlnI
BsaI BsaWI BseAI BsiWI BsoBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI BstEII BstPI Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HaeII HincII HindII HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NdeI NgoMIV NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SapI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Tth111I Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1549 GACGTC GACGT^C
AclI 4264 AACGTT AA^CGTT
AcyI 1549 GACGTC G[GA]^CG[CT]C
AflIII 2626 ACACGT A^C[GA][CT]GT
AhdI 4212 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 19 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4514 CCTCAGG CC^T[ACGT]AGG
ApaI 854 GGGCCC GGGCC^C
AspEI 4212 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 436 GAGCTC G[AT]GC[AT]^C
BamHI 1424 GGATCC G^GATCC
BanI 3388 GGCACC G^G[CT][GA]CC
BlpI 1214 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1214 GCTAAGC GC^T[ACGT]AGC
BsaAI 214 TACGTG [CT]AC^GT[GA]
BsaBI 1746 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1549 GACGTC G[GA]^CG[CT]C
BseRI 1128 CTCCTC GAGGAG(10/8)
BsiEI 28 CGACCG CG[GA][CT]^CG
BsiHKAI 436 GAGCTC G[AT]GC[AT]^C
BsmBI 120 GAGACG CGTCTC(1/5)
BspHI 893 TCATGA T^CATGA
BstAPI 18 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 4514 CCTCAGG CC^T[ACGT]AGG
CelII 1214 GCTAAGC GC^T[ACGT]AGC
DrdI 759 GACAGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4949 CCATGG C^C[GA][CT]GG
Eam1105I 4212 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4514 CCTCAGG CC^T[ACGT]AGG
EcoNI 3268 CCTTGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 1549 GACGTC G[GA]^CG[CT]C
HindIII 402 AAGCTT A^AGCTT
MamI 1746 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 4949 CCATGG C^CATGG
NheI 4597 GCTAGC G^CTAGC
Psp1406I 4264 AACGTT AA^CGTT
RcaI 893 TCATGA T^CATGA
SacI 436 GAGCTC GAGCT^C
SbfI 752 CCTGCAGG CCTGCA^GG
Sse8387I 752 CCTGCAGG CCTGCA^GG
StuI 1053 AGGCCT AGG^CCT
SwaI 3439 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccII 97 CGCG CG^CG
124 CGCG
AflII 4161 CTTAAG C^TTAAG
4778 CTTAAG
BalI 1480 TGGCCA TGG^CCA
1940 TGGCCA
BclI 1836 TGATCA T^GATCA
3014 TGATCA
BcnI 31 CCGGG CC^[GC]GG
858 CCCGG
BfrI 4161 CTTAAG C^TTAAG
4778 CTTAAG
BsmFI 66 GGGAC GGGAC(10/14)
1921 GGGAC
Bsp1407I 1658 TGTACA T^GTACA
2453 TGTACA
BsrGI 1658 TGTACA T^GTACA
2453 TGTACA
Bst1107I 3830 GTATAC GTA^TAC
4612 GTATAC
BstBI 1066 TTCGAA TT^CGAA
2878 TTCGAA
BstF5I 2008 GGATG GGATG(2/0)
2384 GGATG
BstUI 97 CGCG CG^CG
124 CGCG
BstXI 1483 CCACTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3643 CCAGCTATCTGG
BstYI 112 AGATCC [GA]^GATC[CT]
1424 GGATCC
BstZ17I 3830 GTATAC GTA^TAC
4612 GTATAC
DraIII 994 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3735 CACTAAGTG
EaeI 1480 TGGCCA [CT]^GGCC[GA]
1940 TGGCCA
EcoRI 1536 GAATTC G^AATTC
4373 GAATTC
EcoT14I 1916 CCTTGG C^C[AT][AT]GG
4949 CCATGG
EcoT22I 885 ATGCAT ATGCA^T
1206 ATGCAT
FbaI 1836 TGATCA T^GATCA
3014 TGATCA
FokI 2008 GGATG GGATG(9/13)
2384 GGATG
HapII 31 CCGG C^CGG
859 CCGG
HgaI 122 GACGC GACGC(5/10)
1817 GCGTC
HpaII 31 CCGG C^CGG
859 CCGG
MfeI 877 CAATTG C^AATTG
2153 CAATTG
MflI 112 AGATCC [GA]^GATC[CT]
1424 GGATCC
MluNI 1480 TGGCCA TGG^CCA
1940 TGGCCA
MscI 1480 TGGCCA TGG^CCA
1940 TGGCCA
MspI 31 CCGG C^CGG
859 CCGG
MunI 877 CAATTG C^AATTG
2153 CAATTG
MvnI 97 CGCG CG^CG
124 CGCG
NciI 31 CCGGG CC^[GC]GG
858 CCCGG
NsiI 885 ATGCAT ATGCA^T
1206 ATGCAT
NspV 1066 TTCGAA TT^CGAA
2878 TTCGAA
PshAI 1545 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4947 GACCATGGTC
PstI 753 CTGCAG CTGCA^G
3636 CTGCAG
PvuII 1471 CAGCTG CAG^CTG
1785 CAGCTG
SexAI 3463 ACCTGGT A^CC[AT]GGT
3519 ACCAGGT
SfuI 1066 TTCGAA TT^CGAA
2878 TTCGAA
SspBI 1658 TGTACA T^GTACA
2453 TGTACA
StyI 1916 CCTTGG C^C[AT][AT]GG
4949 CCATGG
XcmI 1145 CCATACAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1877 CCATTCCAGTTGTGG
XhoII 112 AGATCC [GA]^GATC[CT]
1424 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsgI
BsiYI BslI BsmAI BsmI Bsp1286I
BspMI BsrDI BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
FauI Fnu4HI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XmnI