##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Alw44I AlwNI
ApaI ApaLI AscI Asp700I Asp718I
AspI AvaI BcgI BfrI BglI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspEI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I CelII CpoI
DraIII DrdI EagI EclXI Eco52I
EcoRV FauI FseI KpnI KspI
MluI MroI MvnI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcyI 515 GGCGCC G[GA]^CG[CT]C
AhdI 1231 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1343 CCTAAGG CC^T[ACGT]AGG
Aor51HI 830 AGCGCT AGC^GCT
AspEI 1231 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3799 TGCGCA TGC^GCA
BalI 3567 TGGCCA TGG^CCA
BanII 3192 GGGCTC G[GA]GC[CT]^C
BbeI 515 GGCGCC GGCGC^C
BbrPI 656 CACGTG CAC^GTG
BciVI 2127 GTATCC GTATCC(6/5)
BcnI 1870 CCCGG CC^[GC]GG
BglII 1812 AGATCT A^GATCT
BsaBI 261 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 515 GGCGCC G[GA]^CG[CT]C
BsmBI 454 GAGACG CGTCTC(1/5)
BspDI 877 ATCGAT AT^CGAT
BspHI 450 TCATGA T^CATGA
BsrFI 486 GCCGGC [GA]^CCGG[CT]
BstEII 3946 GGTGACC G^GT[ACGT]ACC
BstPI 3946 GGTGACC G^GT[ACGT]ACC
Bsu36I 1343 CCTAAGG CC^T[ACGT]AGG
Cfr10I 486 GCCGGC [GA]^CCGG[CT]
ClaI 877 ATCGAT AT^CGAT
EaeI 3567 TGGCCA [CT]^GGCC[GA]
Eam1105I 1231 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 830 AGCGCT AGC^GCT
Eco81I 1343 CCTAAGG CC^T[ACGT]AGG
EcoNI 2149 CCTAAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3946 GGTGACC G^GT[ACGT]ACC
FspI 3799 TGCGCA TGC^GCA
HgaI 456 GACGC GACGC(5/10)
Hin1I 515 GGCGCC G[GA]^CG[CT]C
HpaI 56 GTTAAC GTT^AAC
KasI 515 GGCGCC G^GCGCC
MamI 261 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2461 CAATTG C^AATTG
MluNI 3567 TGGCCA TGG^CCA
MscI 3567 TGGCCA TGG^CCA
MspA1I 1350 CAGCTG C[AC]G^C[GT]G
MunI 2461 CAATTG C^AATTG
NaeI 486 GCCGGC GCC^GGC
NarI 515 GGCGCC GG^CGCC
NciI 1870 CCCGG CC^[GC]GG
NgoMIV 486 GCCGGC G^CCGGC
PmaCI 656 CACGTG CAC^GTG
PmlI 656 CACGTG CAC^GTG
PstI 2450 CTGCAG CTGCA^G
PvuII 1350 CAGCTG CAG^CTG
RcaI 450 TCATGA T^CATGA
ScaI 2967 AGTACT AGT^ACT
SfoI 515 GGCGCC GGC^GCC
SphI 4552 GCATGC GCATG^C
StuI 3932 AGGCCT AGG^CCT
XcmI 4704 CCATGAAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 418 GCGG CCGC(-3/-1)
1079 GCGG
BamHI 266 GGATCC G^GATCC
316 GGATCC
BbvCI 841 CCTCAGC CCTCAGC(-5/-2)
1352 GCTGAGG
BsaAI 656 CACGTG [CT]AC^GT[GA]
1271 CACGTA
BsgI 1512 GTGCAG GTGCAG(16/14)
4354 GTGCAG
BsmI 2384 GCATTC GAATGC(1/-1)
3389 GCATTC
BspLU11I 3322 ACATGT A^CATGT
3329 ACATGT
BsrDI 3163 CATTGC GCAATG(2/0)
4223 CATTGC
DraII 432 AGGGCCT [GA]G^G[ACGT]CC[CT]
1795 AGGGCCT
EcoO109I 432 AGGGCCT [GA]G^G[ACGT]CC[CT]
1795 AGGGCCT
EcoRI 67 GAATTC G^AATTC
3715 GAATTC
HapII 487 CCGG C^CGG
1871 CCGG
HincII 56 GTTAAC GT[CT]^[GA]AC
1130 GTCAAC
HindII 56 GTTAAC GT[CT]^[GA]AC
1130 GTCAAC
HpaII 487 CCGG C^CGG
1871 CCGG
MspI 487 CCGG C^CGG
1871 CCGG
NcoI 523 CCATGG C^CATGG
2131 CCATGG
NdeI 234 CATATG CA^TATG
2750 CATATG
PflMI 1733 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3266 CCAAGCACTGG
PshAI 99 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2026 GACTTTGGTC
Van91I 1733 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3266 CCAAGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AseI AsnI AspHI AvaII
AvrII BanI BbsI BbvI BclI
BfaI BlnI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BssSI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EarI EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII