##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI ApoI AseI AsnI Asp700I
Asp718I AspEI AvrII BamHI BciVI
BclI BfrI BglII BlnI BsaBI
BsiWI BsmI BspDI BspHI BsrDI
Bst1107I BstBI BstZ17I ClaI CpoI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SfuI SnaBI SpeI
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 3453 GACGTC GACGT^C
AccI 3327 GTCGAC GT^[AC][GT]AC
Alw44I 3339 GTGCAC G^TGCAC
ApaLI 3339 GTGCAC G^TGCAC
AscI 201 GGCGCGCC GG^CGCGCC
AspI 4463 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 376 TGCGCA TGC^GCA
Bsp1407I 2857 TGTACA T^GTACA
BspLU11I 3124 ACATGT A^CATGT
BsrGI 2857 TGTACA T^GTACA
BssHII 202 GCGCGC G^CGCGC
BstEII 3835 GGTAACC G^GT[ACGT]ACC
BstPI 3835 GGTAACC G^GT[ACGT]ACC
DrdI 3432 GACACAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3835 GGTAACC G^GT[ACGT]ACC
FspI 376 TGCGCA TGC^GCA
NcoI 1918 CCATGG C^CATGG
NdeI 3295 CATATG CA^TATG
PflFI 4463 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 3234 GAGCTC GAGCT^C
SalI 3327 GTCGAC G^TCGAC
SbfI 2989 CCTGCAGG CCTGCA^GG
ScaI 619 AGTACT AGT^ACT
SgrAI 3686 CGCCGGCG C[GA]^CCGG[CT]G
SphI 3697 GCATGC GCATG^C
Sse8387I 2989 CCTGCAGG CCTGCA^GG
SspBI 2857 TGTACA T^GTACA
TfiI 2452 GAATC G^A[AT]TC
Tsp509I 4178 AATT ^AATT
Tth111I 4463 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 4459 TCCGGA T^CCGGA
4507 TCCGGA
AocI 1417 CCTCAGG CC^T[ACGT]AGG
2867 CCTCAGG
BalI 1411 TGGCCA TGG^CCA
2381 TGGCCA
BcgI 2200 CGAGCTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2229 GCAGAGAAGTCG
BlpI 2807 GCTCAGC GC^T[ACGT]AGC
3470 GCTGAGC
Bpu1102I 2807 GCTCAGC GC^T[ACGT]AGC
3470 GCTGAGC
BseAI 4459 TCCGGA T^CCGGA
4507 TCCGGA
BspEI 4459 TCCGGA T^CCGGA
4507 TCCGGA
BssSI 2760 CACGAG CACGAG(-5/-1)
3024 CTCGTG
BstAPI 2992 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3173 GCAACACGTGC
Bsu36I 1417 CCTCAGG CC^T[ACGT]AGG
2867 CCTCAGG
CelII 2807 GCTCAGC GC^T[ACGT]AGC
3470 GCTGAGC
DraI 2480 TTTAAA TTT^AAA
4180 TTTAAA
Eco81I 1417 CCTCAGG CC^T[ACGT]AGG
2867 CCTCAGG
HincII 354 GTCAAC GT[CT]^[GA]AC
3327 GTCGAC
HindII 354 GTCAAC GT[CT]^[GA]AC
3327 GTCGAC
MluNI 1411 TGGCCA TGG^CCA
2381 TGGCCA
MroI 4459 TCCGGA T^CCGGA
4507 TCCGGA
MscI 1411 TGGCCA TGG^CCA
2381 TGGCCA
PshAI 639 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3311 GACGCGCGTC
SexAI 3396 ACCAGGT A^CC[AT]GGT
3703 ACCTGGT
SfiI 362 GGCCATCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1432 GGCCTGACCGGCC
StuI 1431 AGGCCT AGG^CCT
1795 AGGCCT
XbaI 3979 TCTAGA T^CTAGA
4438 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApaI
AspHI AvaI AvaII BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI