##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI AviII BciVI
BclI BfrI BsaI BsiWI BspHI
Bst1107I BstBI BstZ17I CpoI Eam1105I
EcoRI EcoRV FbaI FseI FspI
KpnI MluI NdeI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SpeI Sse8387I
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflIII 3846 ACATGT A^C[GA][CT]GT
Alw44I 1469 GTGCAC G^TGCAC
Aor51HI 627 AGCGCT AGC^GCT
ApaLI 1469 GTGCAC G^TGCAC
BamHI 611 GGATCC G^GATCC
BbeI 1673 GGCGCC GGCGC^C
BbrPI 4188 CACGTG CAC^GTG
BbsI 210 GAAGAC GAAGAC(2/6)
BglII 1187 AGATCT A^GATCT
BpuAI 210 GAAGAC GAAGAC(2/6)
BsmBI 2400 CGTCTC CGTCTC(1/5)
BsmI 3752 GAATGC GAATGC(1/-1)
Bsp1407I 3836 TGTACA T^GTACA
BspDI 3618 ATCGAT AT^CGAT
BspLU11I 3846 ACATGT A^CATGT
BsrDI 5 GCAATG GCAATG(2/0)
BsrGI 3836 TGTACA T^GTACA
BssHII 72 GCGCGC G^CGCGC
BstEII 1362 GGTGACC G^GT[ACGT]ACC
BstPI 1362 GGTGACC G^GT[ACGT]ACC
ClaI 3618 ATCGAT AT^CGAT
DraI 4008 TTTAAA TTT^AAA
DraIII 2483 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3216 GACCAGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 627 AGCGCT AGC^GCT
EcoO65I 1362 GGTGACC G^GT[ACGT]ACC
EcoT22I 3998 ATGCAT ATGCA^T
HindIII 1987 AAGCTT A^AGCTT
HpaI 3936 GTTAAC GTT^AAC
KasI 1673 GGCGCC G^GCGCC
KspI 3517 CCGCGG CCGC^GG
MfeI 3965 CAATTG C^AATTG
MunI 3965 CAATTG C^AATTG
NarI 1673 GGCGCC GG^CGCC
NsiI 3998 ATGCAT ATGCA^T
PmaCI 4188 CACGTG CAC^GTG
PmlI 4188 CACGTG CAC^GTG
SacII 3517 CCGCGG CCGC^GG
SapI 3255 GAAGAGC GCTCTTC(1/4)
SexAI 1103 ACCAGGT A^CC[AT]GGT
SfoI 1673 GGCGCC GGC^GCC
SmaI 780 CCCGGG CCC^GGG
SnaBI 519 TACGTA TAC^GTA
SphI 11 GCATGC GCATG^C
SspBI 3836 TGTACA T^GTACA
StuI 602 AGGCCT AGG^CCT
XmaCI 780 CCCGGG C^CCGGG
XmaI 780 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 235 GAATTT [GA]^AATT[CT]
4016 GAATTT
ApoI 235 GAATTT [GA]^AATT[CT]
4016 GAATTT
Asp700I 549 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4016 GAATTTATTC
AvrII 2232 CCTAGG C^CTAGG
3450 CCTAGG
BcgI 654 CGATCCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3326 GCACCATGTTCG
BlnI 2232 CCTAGG C^CTAGG
3450 CCTAGG
BsaAI 519 TACGTA [CT]AC^GT[GA]
4188 CACGTG
BsaBI 402 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1701 GATGGCCATC
BsiEI 717 CGGCCG CG[GA][CT]^CG
3023 CGGCCG
BssSI 442 CACGAG CACGAG(-5/-1)
739 CACGAG
BstAPI 588 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3875 GCAGCTCCTGC
BstXI 1047 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1523 CCATGGGGCTGG
EagI 717 CGGCCG C^GGCCG
3023 CGGCCG
EclXI 717 CGGCCG C^GGCCG
3023 CGGCCG
Eco52I 717 CGGCCG C^GGCCG
3023 CGGCCG
MamI 402 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1701 GATGGCCATC
NheI 3120 GCTAGC G^CTAGC
3911 GCTAGC
NspI 11 GCATGC [GA]CATG^[CT]
3846 ACATGT
SacI 1026 GAGCTC GAGCT^C
3664 GAGCTC
TfiI 549 GAATC G^A[AT]TC
2722 GAATC
XmnI 549 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4016 GAATTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII