##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspI BbrPI
BcgI BfrI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BsrBI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoRV FseI HindIII HpaI KspI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfuI SgrAI SmaI
SnaBI SpeI SspI SwaI Tth111I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 1198 GGTACC G^GTACC
AccII 1310 CGCG CG^CG
AhdI 1129 GACAGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1198 GGTACC G^GTACC
AspEI 1129 GACAGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2284 TGCGCA TGC^GCA
AvrII 4587 CCTAGG C^CTAGG
BbeI 1529 GGCGCC GGCGC^C
BciVI 81 GTATCC GTATCC(6/5)
BlnI 4587 CCTAGG C^CTAGG
BsaBI 1367 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1079 GAATGC GAATGC(1/-1)
BstEII 2767 GGTGACC G^GT[ACGT]ACC
BstPI 2767 GGTGACC G^GT[ACGT]ACC
BstUI 1310 CGCG CG^CG
DraI 220 TTTAAA TTT^AAA
DraIII 2930 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1539 GACCCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1129 GACAGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2767 GGTGACC G^GT[ACGT]ACC
EcoRI 3957 GAATTC G^AATTC
FspI 2284 TGCGCA TGC^GCA
KasI 1529 GGCGCC G^GCGCC
KpnI 1198 GGTACC GGTAC^C
MamI 1367 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1310 CGCG CG^CG
NaeI 2203 GCCGGC GCC^GGC
NarI 1529 GGCGCC GG^CGCC
NgoMIV 2203 GCCGGC G^CCGGC
PaeR7I 1018 CTCGAG C^TCGAG
SapI 1670 GCTCTTC GCTCTTC(1/4)
ScaI 2186 AGTACT AGT^ACT
SexAI 1201 ACCAGGT A^CC[AT]GGT
SfiI 2682 GGCCAAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1529 GGCGCC GGC^GCC
XhoI 1018 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1185 GAATTT [GA]^AATT[CT]
3957 GAATTC
AcyI 1455 GACGCC G[GA]^CG[CT]C
1529 GGCGCC
AflIII 246 ACATGT A^C[GA][CT]GT
342 ACATGT
AocI 2621 CCTCAGG CC^T[ACGT]AGG
3417 CCTGAGG
ApoI 1185 GAATTT [GA]^AATT[CT]
3957 GAATTC
BamHI 3598 GGATCC G^GATCC
3856 GGATCC
BbvCI 1826 GCTGAGG CCTCAGC(-5/-2)
2211 GCTGAGG
BclI 535 TGATCA T^GATCA
1372 TGATCA
BsaAI 284 TACGTG [CT]AC^GT[GA]
2378 TACGTG
BsaHI 1455 GACGCC G[GA]^CG[CT]C
1529 GGCGCC
BsaWI 1060 ACCGGA [AT]^CCGG[AT]
1489 ACCGGA
BsgI 822 CTGCAC GTGCAG(16/14)
3612 CTGCAC
Bsp1407I 1846 TGTACA T^GTACA
2248 TGTACA
BspLU11I 246 ACATGT A^CATGT
342 ACATGT
BsrFI 2203 GCCGGC [GA]^CCGG[CT]
3122 ACCGGC
BsrGI 1846 TGTACA T^GTACA
2248 TGTACA
BssSI 2385 CACGAG CACGAG(-5/-1)
1806 CTCGTG
BstAPI 2606 GCATCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4050 GCACCCAATGC
Bsu36I 2621 CCTCAGG CC^T[ACGT]AGG
3417 CCTGAGG
Cfr10I 2203 GCCGGC [GA]^CCGG[CT]
3122 ACCGGC
Eco81I 2621 CCTCAGG CC^T[ACGT]AGG
3417 CCTGAGG
EcoT22I 2292 ATGCAT ATGCA^T
4057 ATGCAT
FbaI 535 TGATCA T^GATCA
1372 TGATCA
Hin1I 1455 GACGCC G[GA]^CG[CT]C
1529 GGCGCC
NsiI 2292 ATGCAT ATGCA^T
4057 ATGCAT
PshAI 3476 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4520 GACTCACGTC
SspBI 1846 TGTACA T^GTACA
2248 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI SphI Sse8387I StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII