##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AgeI AscI Asp718I AspI AviII
AvrII BbeI BclI BglII BlnI
BsaHI BsiWI Bsp1407I BspDI BspLU11I
BsrFI BsrGI BssHII BstBI BstEII
BstPI Cfr10I ClaI CpoI DraIII
DrdI DsaI EcoO65I EcoRV FbaI
FseI FspI HgaI Hin1I KasI
KpnI KspI MluI NaeI NarI
NcoI NgoMIV NotI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 690 TCCGGA T^CCGGA
Alw44I 2637 GTGCAC G^TGCAC
ApaI 3703 GGGCCC GGGCC^C
ApaLI 2637 GTGCAC G^TGCAC
AseI 2272 ATTAAT AT^TAAT
AsnI 2272 ATTAAT AT^TAAT
AvaI 75 CTCGGG C^[CT]CG[GA]G
BamHI 2393 GGATCC G^GATCC
BanI 1403 GGCACC G^G[CT][GA]CC
BbrPI 572 CACGTG CAC^GTG
BbsI 3831 GAAGAC GAAGAC(2/6)
BglI 1157 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3831 GAAGAC GAAGAC(2/6)
BsaAI 572 CACGTG [CT]AC^GT[GA]
BsaWI 690 TCCGGA [AT]^CCGG[AT]
BseAI 690 TCCGGA T^CCGGA
BsiEI 852 CGGCCG CG[GA][CT]^CG
BsmBI 1366 CGTCTC CGTCTC(1/5)
BsmI 1276 GAATGC GAATGC(1/-1)
BsoBI 75 CTCGGG C^[CT]CG[GA]G
BspEI 690 TCCGGA T^CCGGA
BspMI 1095 ACCTGC ACCTGC(4/8)
BsrBI 3247 GAGCGG CCGCTC(-3/-3)
BssSI 1590 CTCGTG CACGAG(-5/-1)
Bst1107I 3508 GTATAC GTA^TAC
BstAPI 1252 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3508 GTATAC GTA^TAC
EagI 852 CGGCCG C^GGCCG
EclXI 852 CGGCCG C^GGCCG
Eco52I 852 CGGCCG C^GGCCG
EcoRI 4073 GAATTC G^AATTC
EcoT22I 1902 ATGCAT ATGCA^T
MroI 690 TCCGGA T^CCGGA
NdeI 19 CATATG CA^TATG
NruI 1356 TCGCGA TCG^CGA
NsiI 1902 ATGCAT ATGCA^T
PflMI 595 CCATTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 572 CACGTG CAC^GTG
PmlI 572 CACGTG CAC^GTG
PshBI 2272 ATTAAT AT^TAAT
SfiI 1156 GGCCTACCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 355 ACTAGT A^CTAGT
SphI 621 GCATGC GCATG^C
StuI 1164 AGGCCT AGG^CCT
Van91I 595 CCATTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2703 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AflII 163 CTTAAG C^TTAAG
4263 CTTAAG
AhdI 1997 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3097 GACATCAGGTC
AocI 2097 CCTGAGG CC^T[ACGT]AGG
2848 CCTGAGG
Aor51HI 785 AGCGCT AGC^GCT
1173 AGCGCT
AspEI 1997 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3097 GACATCAGGTC
BalI 1134 TGGCCA TGG^CCA
3609 TGGCCA
BbvCI 2404 CCTCAGC CCTCAGC(-5/-2)
974 GCTGAGG
BcgI 712 CGACAACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
463 GCAGTATCATCG
BciVI 955 GGATAC GTATCC(6/5)
2917 GGATAC
BcnI 223 CCCGG CC^[GC]GG
2422 CCGGG
BfrI 163 CTTAAG C^TTAAG
4263 CTTAAG
BlpI 965 GCTCAGC GC^T[ACGT]AGC
3875 GCTCAGC
Bpu1102I 965 GCTCAGC GC^T[ACGT]AGC
3875 GCTCAGC
BsaBI 545 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4047 GATCTAAATC
BseRI 743 GAGGAG GAGGAG(10/8)
1145 GAGGAG
BsgI 998 GTGCAG GTGCAG(16/14)
2228 GTGCAG
BsrDI 596 CATTGC GCAATG(2/0)
718 CATTGC
BstYI 2393 GGATCC [GA]^GATC[CT]
4046 GGATCT
Bsu36I 2097 CCTGAGG CC^T[ACGT]AGG
2848 CCTGAGG
CelII 965 GCTCAGC GC^T[ACGT]AGC
3875 GCTCAGC
Eam1105I 1997 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3097 GACATCAGGTC
Eco47III 785 AGCGCT AGC^GCT
1173 AGCGCT
Eco81I 2097 CCTGAGG CC^T[ACGT]AGG
2848 CCTGAGG
HincII 1680 GTTAAC GT[CT]^[GA]AC
4001 GTTAAC
HindII 1680 GTTAAC GT[CT]^[GA]AC
4001 GTTAAC
HindIII 2550 AAGCTT A^AGCTT
3267 AAGCTT
HpaI 1680 GTTAAC GTT^AAC
4001 GTTAAC
MamI 545 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4047 GATCTAAATC
MfeI 1856 CAATTG C^AATTG
3292 CAATTG
MflI 2393 GGATCC [GA]^GATC[CT]
4046 GGATCT
MluNI 1134 TGGCCA TGG^CCA
3609 TGGCCA
MscI 1134 TGGCCA TGG^CCA
3609 TGGCCA
MunI 1856 CAATTG C^AATTG
3292 CAATTG
NciI 223 CCCGG CC^[GC]GG
2422 CCGGG
NheI 92 GCTAGC G^CTAGC
2202 GCTAGC
NspI 621 GCATGC [GA]CATG^[CT]
3069 GCATGT
PpuMI 2003 AGGTCCC [GA]G^G[AT]CC[CT]
3103 AGGTCCC
SapI 3422 GAAGAGC GCTCTTC(1/4)
3145 GCTCTTC
XhoII 2393 GGATCC [GA]^GATC[CT]
4046 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanII BbvI BfaI
BmyI BpmI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI