##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AgeI AhdI Alw44I ApaLI AscI
AspEI AviII BciVI BclI BsiEI
BsiWI BspDI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoRV FbaI
FseI FspI HindIII HpaI KspI
MluI NdeI NheI NotI NruI
NspV PacI PinAI PmeI PshAI
PvuI RsrII SacII SalI SfiI
SfuI SgrAI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 319 GACGTC GACGT^C
AccIII 101 TCCGGA T^CCGGA
AflII 4787 CTTAAG C^TTAAG
Aor51HI 4552 AGCGCT AGC^GCT
AseI 1066 ATTAAT AT^TAAT
AsnI 1066 ATTAAT AT^TAAT
AspHI 734 GAGCTC G[AT]GC[AT]^C
AspI 3129 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3675 CCTAGG C^CTAGG
BbeI 3603 GGCGCC GGCGC^C
BfrI 4787 CTTAAG C^TTAAG
BglII 5612 AGATCT A^GATCT
BlnI 3675 CCTAGG C^CTAGG
BlpI 5750 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5750 GCTCAGC GC^T[ACGT]AGC
BsaBI 5404 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 101 TCCGGA [AT]^CCGG[AT]
BseAI 101 TCCGGA T^CCGGA
BsiHKAI 734 GAGCTC G[AT]GC[AT]^C
BsmBI 4224 GAGACG CGTCTC(1/5)
BspEI 101 TCCGGA T^CCGGA
BspHI 1270 TCATGA T^CATGA
BstXI 2653 CCACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 5750 GCTCAGC GC^T[ACGT]AGC
DraIII 2137 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4552 AGCGCT AGC^GCT
FauI 5300 CCCGC CCCGC(4/6)
KasI 3603 GGCGCC G^GCGCC
MamI 5404 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4097 CAATTG C^AATTG
MroI 101 TCCGGA T^CCGGA
MunI 4097 CAATTG C^AATTG
NaeI 227 GCCGGC GCC^GGC
NarI 3603 GGCGCC GG^CGCC
NgoMIV 227 GCCGGC G^CCGGC
PaeR7I 2795 CTCGAG C^TCGAG
PflFI 3129 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1066 ATTAAT AT^TAAT
RcaI 1270 TCATGA T^CATGA
SacI 734 GAGCTC GAGCT^C
SbfI 3294 CCTGCAGG CCTGCA^GG
ScaI 2900 AGTACT AGT^ACT
SexAI 3130 ACCAGGT A^CC[AT]GGT
SfoI 3603 GGCGCC GGC^GCC
SpeI 5121 ACTAGT A^CTAGT
SphI 1691 GCATGC GCATG^C
Sse8387I 3294 CCTGCAGG CCTGCA^GG
SspI 4711 AATATT AAT^ATT
Tth111I 3129 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2795 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 2565 GGTACC G^GTACC
6336 GGTACC
AclI 3846 AACGTT AA^CGTT
6134 AACGTT
ApaI 621 GGGCCC GGGCC^C
3783 GGGCCC
Asp718I 2565 GGTACC G^GTACC
6336 GGTACC
BbrPI 5393 CACGTG CAC^GTG
5883 CACGTG
BcgI 1460 CGATTTTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1596 CGAGGAAAATGC
BsrBI 5301 CCGCTC CCGCTC(-3/-3)
2482 GAGCGG
BstAPI 1572 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5741 GCAGTGTCTGC
BstEII 1895 GGTCACC G^GT[ACGT]ACC
2245 GGTCACC
BstPI 1895 GGTCACC G^GT[ACGT]ACC
2245 GGTCACC
DraI 673 TTTAAA TTT^AAA
4082 TTTAAA
EcoO65I 1895 GGTCACC G^GT[ACGT]ACC
2245 GGTCACC
EcoT22I 4436 ATGCAT ATGCA^T
6500 ATGCAT
KpnI 2565 GGTACC GGTAC^C
6336 GGTACC
NsiI 4436 ATGCAT ATGCA^T
6500 ATGCAT
PflMI 1993 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2814 CCACCCTTTGG
PmaCI 5393 CACGTG CAC^GTG
5883 CACGTG
PmlI 5393 CACGTG CAC^GTG
5883 CACGTG
Psp1406I 3846 AACGTT AA^CGTT
6134 AACGTT
SapI 731 GAAGAGC GCTCTTC(1/4)
2801 GCTCTTC
SnaBI 4851 TACGTA TAC^GTA
6259 TACGTA
Van91I 1993 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2814 CCACCCTTTGG
XbaI 1758 TCTAGA T^CTAGA
2016 TCTAGA
XcmI 4513 CCAGTGTCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5655 CCAGTTTGAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI Asp700I AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI
XmnI