##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AclI AgeI AhdI AscI
AspEI AspI AvrII BbrPI BcgI
BlnI BlpI Bpu1102I BsaBI BsiWI
BsmBI BsmI BspDI BssHII BssSI
BstBI CelII ClaI CpoI Eam1105I
EcoRI EcoRV EcoT22I FseI KspI
MamI MfeI MluI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SfuI SgrAI
SnaBI SpeI SphI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflII 4560 CTTAAG C^TTAAG
AflIII 4946 ACATGT A^C[GA][CT]GT
Alw44I 1707 GTGCAC G^TGCAC
Aor51HI 1695 AGCGCT AGC^GCT
ApaLI 1707 GTGCAC G^TGCAC
AseI 2812 ATTAAT AT^TAAT
AsnI 2812 ATTAAT AT^TAAT
AviII 531 TGCGCA TGC^GCA
BamHI 220 GGATCC G^GATCC
BfrI 4560 CTTAAG C^TTAAG
BglII 3132 AGATCT A^GATCT
BsgI 3735 CTGCAC GTGCAG(16/14)
BsiEI 605 CGGCCG CG[GA][CT]^CG
Bsp1407I 1174 TGTACA T^GTACA
BspLU11I 4946 ACATGT A^CATGT
BsrFI 129 ACCGGC [GA]^CCGG[CT]
BsrGI 1174 TGTACA T^GTACA
BstXI 5581 CCAAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 129 ACCGGC [GA]^CCGG[CT]
DrdI 1790 GACAGATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 605 CGGCCG C^GGCCG
EclXI 605 CGGCCG C^GGCCG
Eco47III 1695 AGCGCT AGC^GCT
Eco52I 605 CGGCCG C^GGCCG
EcoNI 812 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 531 TGCGCA TGC^GCA
NdeI 2852 CATATG CA^TATG
PflMI 1447 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4787 GACGCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2812 ATTAAT AT^TAAT
SbfI 306 CCTGCAGG CCTGCA^GG
SfiI 303 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 4560 CTTAAG C^T[CT][GA]AG
Sse8387I 306 CCTGCAGG CCTGCA^GG
SspBI 1174 TGTACA T^GTACA
StuI 1008 AGGCCT AGG^CCT
Van91I 1447 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1193 GTATAC GT^[AC][GT]AC
3531 GTATAC
AccIII 217 TCCGGA T^CCGGA
814 TCCGGA
Asp700I 1457 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5137 GAAAAGATTC
BbeI 383 GGCGCC GGCGC^C
549 GGCGCC
BbvCI 3653 CCTCAGC CCTCAGC(-5/-2)
5097 GCTGAGG
BciVI 719 GGATAC GTATCC(6/5)
4500 GGATAC
BclI 1147 TGATCA T^GATCA
1228 TGATCA
BsaAI 669 TACGTG [CT]AC^GT[GA]
3410 TACGTG
BsaWI 217 TCCGGA [AT]^CCGG[AT]
814 TCCGGA
BseAI 217 TCCGGA T^CCGGA
814 TCCGGA
BspEI 217 TCCGGA T^CCGGA
814 TCCGGA
BspHI 1807 TCATGA T^CATGA
4641 TCATGA
BsrBI 267 CCGCTC CCGCTC(-3/-3)
3397 CCGCTC
Bst1107I 1193 GTATAC GTA^TAC
3531 GTATAC
BstZ17I 1193 GTATAC GTA^TAC
3531 GTATAC
DraIII 3211 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3738 CACTTGGTG
FbaI 1147 TGATCA T^GATCA
1228 TGATCA
HindIII 2369 AAGCTT A^AGCTT
5666 AAGCTT
HpaI 5053 GTTAAC GTT^AAC
5784 GTTAAC
KasI 383 GGCGCC G^GCGCC
549 GGCGCC
MroI 217 TCCGGA T^CCGGA
814 TCCGGA
NarI 383 GGCGCC GG^CGCC
549 GGCGCC
NspI 4195 GCATGT [GA]CATG^[CT]
4946 ACATGT
RcaI 1807 TCATGA T^CATGA
4641 TCATGA
ScaI 2303 AGTACT AGT^ACT
4718 AGTACT
SfoI 383 GGCGCC GGC^GCC
549 GGCGCC
SspI 5746 AATATT AAT^ATT
5797 AATATT
TaqI 1162 TCGA T^CGA
3958 TCGA
TthHB8I 1162 TCGA T^CGA
3958 TCGA
XmnI 1457 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5137 GAAAAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp718I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmaI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII XmaCI XmaI