##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspMI BsrGI BssHII
Bst1107I BstEII BstPI BstZ17I CfoI
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FseI FspI HaeII HhaI HinP1I
HpaI KasI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI Tth111I Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 40 GACGCC G[GA]^CG[CT]C
AlwI 1051 GATCC GGATC(4/5)
AocI 2045 CCTCAGG CC^T[ACGT]AGG
AseI 2884 ATTAAT AT^TAAT
AsnI 2884 ATTAAT AT^TAAT
AvaI 1287 CCCGAG C^[CT]CG[GA]G
BalI 246 TGGCCA TGG^CCA
BclI 1708 TGATCA T^GATCA
BlpI 63 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC
BsaHI 40 GACGCC G[GA]^CG[CT]C
BsaI 119 GAGACC GGTCTC(1/5)
BsgI 698 GTGCAG GTGCAG(16/14)
BsiEI 57 CGACCG CG[GA][CT]^CG
BsoBI 1287 CCCGAG C^[CT]CG[GA]G
BspHI 1628 TCATGA T^CATGA
BspLU11I 2377 ACATGT A^CATGT
BsrBI 1154 CCGCTC CCGCTC(-3/-3)
BsrDI 718 GCAATG GCAATG(2/0)
BsrFI 59 ACCGGC [GA]^CCGG[CT]
BssSI 2217 CACGAG CACGAG(-5/-1)
BstAPI 1494 GCAGCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 211 TTCGAA TT^CGAA
BstXI 249 CCATAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1050 AGATCC [GA]^GATC[CT]
Bsu36I 2045 CCTCAGG CC^T[ACGT]AGG
CelII 63 GCTGAGC GC^T[ACGT]AGC
Cfr10I 59 ACCGGC [GA]^CCGG[CT]
EaeI 246 TGGCCA [CT]^GGCC[GA]
Eco81I 2045 CCTCAGG CC^T[ACGT]AGG
EcoNI 766 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1482 GATATC GAT^ATC
FbaI 1708 TGATCA T^GATCA
Hin1I 40 GACGCC G[GA]^CG[CT]C
MflI 1050 AGATCC [GA]^GATC[CT]
MluNI 246 TGGCCA TGG^CCA
MscI 246 TGGCCA TGG^CCA
NspV 211 TTCGAA TT^CGAA
PshBI 2884 ATTAAT AT^TAAT
RcaI 1628 TCATGA T^CATGA
ScaI 2513 AGTACT AGT^ACT
SfuI 211 TTCGAA TT^CGAA
SspI 2523 AATATT AAT^ATT
SwaI 1666 ATTTAAAT ATTT^AAAT
XbaI 1653 TCTAGA T^CTAGA
XhoII 1050 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 2631 GGTACC G^GTACC
2729 GGTACC
AccI 290 GTCTAC GT^[AC][GT]AC
1871 GTCTAC
AccII 133 CGCG CG^CG
1605 CGCG
AflIII 694 ACGTGT A^C[GA][CT]GT
2377 ACATGT
AlwNI 1992 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2257 CAGAGTCTG
ApaI 96 GGGCCC GGGCC^C
1417 GGGCCC
Asp718I 2631 GGTACC G^GTACC
2729 GGTACC
AspHI 49 GAGCAC G[AT]GC[AT]^C
1736 GTGCTC
BanI 2631 GGTACC G^G[CT][GA]CC
2729 GGTACC
BbvCI 1331 GCTGAGG CCTCAGC(-5/-2)
2782 GCTGAGG
BcnI 94 CCGGG CC^[GC]GG
115 CCCGG
BpmI 767 CTGGAG CTGGAG(16/14)
946 CTGGAG
BsiHKAI 49 GAGCAC G[AT]GC[AT]^C
1736 GTGCTC
BsmI 2004 GAATGC GAATGC(1/-1)
1248 GCATTC
BstUI 133 CGCG CG^CG
1605 CGCG
EcoT14I 2158 CCATGG C^C[AT][AT]GG
2466 CCATGG
EcoT22I 1720 ATGCAT ATGCA^T
2440 ATGCAT
HgaI 40 GACGC GACGC(5/10)
68 GCGTC
HincII 645 GTTGAC GT[CT]^[GA]AC
2802 GTTGAC
HindII 645 GTTGAC GT[CT]^[GA]AC
2802 GTTGAC
HindIII 407 AAGCTT A^AGCTT
2767 AAGCTT
HphI 1524 GGTGA GGTGA(8/7)
2149 TCACC
KpnI 2631 GGTACC GGTAC^C
2729 GGTACC
MspA1I 190 CAGCGG C[AC]G^C[GT]G
2758 CAGCGG
MvnI 133 CGCG CG^CG
1605 CGCG
NciI 94 CCGGG CC^[GC]GG
115 CCCGG
NcoI 2158 CCATGG C^CATGG
2466 CCATGG
NsiI 1720 ATGCAT ATGCA^T
2440 ATGCAT
PpuMI 774 AGGACCT [GA]G^G[AT]CC[CT]
1283 GGGACCC
SapI 313 GAAGAGC GCTCTTC(1/4)
1123 GAAGAGC
StyI 2158 CCATGG C^C[AT][AT]GG
2466 CCATGG
Tsp45I 274 GTGAC ^GT[GC]AC
2923 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AvaII BanII BbsI BbvI
BfaI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XmnI