##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AviII
BbrPI BcgI BciVI BclI BglI
BlpI Bpu1102I BsiEI BsiWI Bsp1407I
BspDI BspHI BsrGI BssHII Bst1107I
BstZ17I CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoT22I FbaI FseI FspI KpnI
MluI NaeI NcoI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PvuI PvuII RcaI RsrII SalI
SbfI ScaI SexAI SfiI SgrAI
SmaI SnaBI Sse8387I SspBI Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 221 TCCGGA T^CCGGA
AclI 3739 AACGTT AA^CGTT
AcyI 92 GGCGCC G[GA]^CG[CT]C
Asp700I 904 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 6538 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3272 CCCGAG C^[CT]CG[GA]G
AvrII 2422 CCTAGG C^CTAGG
BanII 5988 GAGCTC G[GA]GC[CT]^C
BbeI 92 GGCGCC GGCGC^C
BglII 4151 AGATCT A^GATCT
BlnI 2422 CCTAGG C^CTAGG
BsaBI 5036 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 92 GGCGCC G[GA]^CG[CT]C
BseAI 221 TCCGGA T^CCGGA
BsoBI 3272 CCCGAG C^[CT]CG[GA]G
BspEI 221 TCCGGA T^CCGGA
BspLU11I 3923 ACATGT A^CATGT
BspMI 1251 GCAGGT ACCTGC(4/8)
BsrFI 154 ACCGGC [GA]^CCGG[CT]
BstBI 130 TTCGAA TT^CGAA
Cfr10I 154 ACCGGC [GA]^CCGG[CT]
Hin1I 92 GGCGCC G[GA]^CG[CT]C
KasI 92 GGCGCC G^GCGCC
KspI 202 CCGCGG CCGC^GG
MamI 5036 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 221 TCCGGA T^CCGGA
MspA1I 202 CCGCGG C[AC]G^C[GT]G
NarI 92 GGCGCC GG^CGCC
NdeI 6115 CATATG CA^TATG
NspV 130 TTCGAA TT^CGAA
PflFI 6538 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4397 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3739 AACGTT AA^CGTT
PstI 1246 CTGCAG CTGCA^G
SacI 5988 GAGCTC GAGCT^C
SacII 202 CCGCGG CCGC^GG
SapI 48 GAAGAGC GCTCTTC(1/4)
SfoI 92 GGCGCC GGC^GCC
SfuI 130 TTCGAA TT^CGAA
SpeI 2043 ACTAGT A^CTAGT
SphI 2083 GCATGC GCATG^C
SwaI 4878 ATTTAAAT ATTT^AAAT
Tth111I 6538 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4180 TCTAGA T^CTAGA
XcmI 2813 CCAGGTTTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 904 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 377 ACGTGT A^C[GA][CT]GT
3923 ACATGT
Alw44I 414 GTGCAC G^TGCAC
3477 GTGCAC
AlwNI 814 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
5453 CAGATTCTG
ApaLI 414 GTGCAC G^TGCAC
3477 GTGCAC
BamHI 736 GGATCC G^GATCC
790 GGATCC
BcnI 3444 CCCGG CC^[GC]GG
5538 CCGGG
BsaAI 256 TACGTG [CT]AC^GT[GA]
4699 TACGTG
BsaWI 221 TCCGGA [AT]^CCGG[AT]
5308 TCCGGT
BsgI 6510 CTGCAC GTGCAG(16/14)
3210 GTGCAG
BsmBI 6376 CGTCTC CGTCTC(1/5)
6543 CGTCTC
BsmI 6104 GAATGC GAATGC(1/-1)
5622 GCATTC
BsrDI 5150 GCAATG GCAATG(2/0)
6108 GCAATG
BssSI 6329 CACGAG CACGAG(-5/-1)
1205 CTCGTG
BstAPI 1195 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3236 GCAACCTCTGC
BstEII 150 GGTGACC G^GT[ACGT]ACC
1111 GGTGACC
BstPI 150 GGTGACC G^GT[ACGT]ACC
1111 GGTGACC
DraII 74 GGGACCC [GA]G^G[ACGT]CC[CT]
4124 GGGACCT
DrdI 1525 GACACTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5567 GACTTATTGGTC
EcoO109I 74 GGGACCC [GA]G^G[ACGT]CC[CT]
4124 GGGACCT
EcoO65I 150 GGTGACC G^GT[ACGT]ACC
1111 GGTGACC
EcoRV 2428 GATATC GAT^ATC
4716 GATATC
HaeII 13 AGCGCC [GA]GCGC^[CT]
92 GGCGCC
HgaI 98 GCGTC GACGC(5/10)
3694 GCGTC
HpaI 1933 GTTAAC GTT^AAC
4435 GTTAAC
NciI 3444 CCCGG CC^[GC]GG
5538 CCGGG
PleI 5516 GACTC GAGTC(4/5)
6538 GACTC
PpuMI 74 GGGACCC [GA]G^G[AT]CC[CT]
4124 GGGACCT
StuI 694 AGGCCT AGG^CCT
5089 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI AocI ApoI
AseI AsnI AspHI AvaII BalI
BanI BbsI BbvCI BbvI BfaI
BfrI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI Eco81I EcoNI EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspI MunI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII