##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoO65I FseI
FspI HpaI KasI KpnI MamI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI SfiI SfoI SgrAI
SnaBI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 225 GGCGTC G[GA]^CG[CT]C
AflIII 5100 ACATGT A^C[GA][CT]GT
AhdI 2041 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 471 AGCGCT AGC^GCT
AspEI 2041 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 1014 TGATCA T^GATCA
BlpI 2736 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2736 GCTGAGC GC^T[ACGT]AGC
BsaHI 225 GGCGTC G[GA]^CG[CT]C
BsaI 5477 GAGACC GGTCTC(1/5)
Bsp1407I 5107 TGTACA T^GTACA
BspLU11I 5100 ACATGT A^CATGT
BsrDI 5700 GCAATG GCAATG(2/0)
BsrFI 5192 GCCGGT [GA]^CCGG[CT]
BsrGI 5107 TGTACA T^GTACA
BstXI 1295 CCAGGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2736 GCTGAGC GC^T[ACGT]AGC
Cfr10I 5192 GCCGGT [GA]^CCGG[CT]
DraIII 3054 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3012 GACAGTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2041 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 471 AGCGCT AGC^GCT
EcoRI 3044 GAATTC G^AATTC
EcoT22I 4274 ATGCAT ATGCA^T
FbaI 1014 TGATCA T^GATCA
Hin1I 225 GGCGTC G[GA]^CG[CT]C
HindIII 4830 AAGCTT A^AGCTT
KspI 215 CCGCGG CCGC^GG
MfeI 5021 CAATTG C^AATTG
MunI 5021 CAATTG C^AATTG
NsiI 4274 ATGCAT ATGCA^T
PaeR7I 4060 CTCGAG C^TCGAG
PflMI 3000 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 4964 GAGCTC GAGCT^C
SacII 215 CCGCGG CCGC^GG
SapI 3 GAAGAGC GCTCTTC(1/4)
ScaI 4334 AGTACT AGT^ACT
SpeI 4637 ACTAGT A^CTAGT
SspBI 5107 TGTACA T^GTACA
Van91I 3000 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 4060 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 4363 CTTAAG C^TTAAG
4786 CTTAAG
AocI 1749 CCTGAGG CC^T[ACGT]AGG
5421 CCTGAGG
ApaI 392 GGGCCC GGGCC^C
3411 GGGCCC
BamHI 3354 GGATCC G^GATCC
4577 GGATCC
BfrI 4363 CTTAAG C^TTAAG
4786 CTTAAG
BsgI 546 CTGCAC GTGCAG(16/14)
5044 CTGCAC
BsmI 484 GCATTC GAATGC(1/-1)
697 GCATTC
BspHI 1282 TCATGA T^CATGA
2107 TCATGA
Bsu36I 1749 CCTGAGG CC^T[ACGT]AGG
5421 CCTGAGG
DraI 4430 TTTAAA TTT^AAA
5748 TTTAAA
Eco81I 1749 CCTGAGG CC^T[ACGT]AGG
5421 CCTGAGG
EcoRV 1206 GATATC GAT^ATC
3167 GATATC
HincII 1476 GTCAAC GT[CT]^[GA]AC
2487 GTTGAC
HindII 1476 GTCAAC GT[CT]^[GA]AC
2487 GTTGAC
NcoI 2310 CCATGG C^CATGG
5492 CCATGG
RcaI 1282 TCATGA T^CATGA
2107 TCATGA
SphI 2619 GCATGC GCATG^C
2836 GCATGC
SspI 3558 AATATT AAT^ATT
5758 AATATT
XbaI 1596 TCTAGA T^CTAGA
3538 TCTAGA
XcmI 1557 CCATTGACTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2310 CCATGGCTCATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstBI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI NspV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfuI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI XmnI