##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BclI BlnI BsiWI
BspLU11I BssHII Bst1107I BstZ17I CpoI
DrdI Eam1105I Eco47III EcoRV EcoT22I
FbaI FspI HincII HindII HpaI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI PacI PflFI
PmeI PshAI PshBI PvuI RsrII
SalI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 1188 GACGTC GACGT^C
Acc65I 1436 GGTACC G^GTACC
AccII 28 CGCG CG^CG
AccIII 327 TCCGGA T^CCGGA
AclI 3733 AACGTT AA^CGTT
AflII 4564 CTTAAG C^TTAAG
AflIII 500 ACACGT A^C[GA][CT]GT
AgeI 3615 ACCGGT A^CCGGT
Asp700I 476 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1436 GGTACC G^GTACC
BamHI 4136 GGATCC G^GATCC
BbrPI 501 CACGTG CAC^GTG
BcgI 1310 GCAAGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4564 CTTAAG C^TTAAG
BsaAI 501 CACGTG [CT]AC^GT[GA]
BsaBI 694 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 327 TCCGGA T^CCGGA
BsiEI 929 CGGCCG CG[GA][CT]^CG
Bsp1407I 4832 TGTACA T^GTACA
BspDI 524 ATCGAT AT^CGAT
BspEI 327 TCCGGA T^CCGGA
BsrGI 4832 TGTACA T^GTACA
BssSI 2592 CACGAG CACGAG(-5/-1)
BstAPI 3125 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 264 TTCGAA TT^CGAA
BstUI 28 CGCG CG^CG
ClaI 524 ATCGAT AT^CGAT
DraI 4784 TTTAAA TTT^AAA
DraIII 3839 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 929 CGGCCG C^GGCCG
EclXI 929 CGGCCG C^GGCCG
Eco52I 929 CGGCCG C^GGCCG
EcoRI 267 GAATTC G^AATTC
FseI 930 GGCCGGCC GGCCGG^CC
HindIII 2172 AAGCTT A^AGCTT
KpnI 1436 GGTACC GGTAC^C
KspI 27 CCGCGG CCGC^GG
MamI 694 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 327 TCCGGA T^CCGGA
MvnI 28 CGCG CG^CG
NspI 2432 GCATGT [GA]CATG^[CT]
NspV 264 TTCGAA TT^CGAA
PaeR7I 1203 CTCGAG C^TCGAG
PinAI 3615 ACCGGT A^CCGGT
PmaCI 501 CACGTG CAC^GTG
PmlI 501 CACGTG CAC^GTG
Psp1406I 3733 AACGTT AA^CGTT
SacII 27 CCGCGG CCGC^GG
SapI 1281 GAAGAGC GCTCTTC(1/4)
SexAI 3954 ACCTGGT A^CC[AT]GGT
SfiI 82 GGCCATGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 264 TTCGAA TT^CGAA
SgrAI 1607 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 4387 CCCGGG CCC^GGG
SspBI 4832 TGTACA T^GTACA
XhoI 1203 CTCGAG C^TCGAG
XmaCI 4387 CCCGGG C^CCGGG
XmaI 4387 CCCGGG C^CCGGG
XmnI 476 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaI 1203 CTCGAG C^[CT]CG[GA]G
4387 CCCGGG
BalI 1235 TGGCCA TGG^CCA
2727 TGGCCA
BbeI 44 GGCGCC GGCGC^C
2994 GGCGCC
BbsI 144 GAAGAC GAAGAC(2/6)
4312 GAAGAC
BciVI 1531 GGATAC GTATCC(6/5)
4254 GTATCC
BglII 216 AGATCT A^GATCT
917 AGATCT
BpuAI 144 GAAGAC GAAGAC(2/6)
4312 GAAGAC
BsaWI 327 TCCGGA [AT]^CCGG[AT]
3615 ACCGGT
BsmBI 196 GAGACG CGTCTC(1/5)
1186 GAGACG
BsmI 244 GAATGC GAATGC(1/-1)
3350 GCATTC
BsoBI 1203 CTCGAG C^[CT]CG[GA]G
4387 CCCGGG
BspHI 385 TCATGA T^CATGA
513 TCATGA
BsrBI 3892 CCGCTC CCGCTC(-3/-3)
940 GAGCGG
BsrDI 778 CATTGC GCAATG(2/0)
4492 GCAATG
BstXI 1733 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1802 CCACAGACTTGG
HaeII 44 GGCGCC [GA]GCGC^[CT]
2994 GGCGCC
HgaI 4556 GACGC GACGC(5/10)
2605 GCGTC
KasI 44 GGCGCC G^GCGCC
2994 GGCGCC
MluNI 1235 TGGCCA TGG^CCA
2727 TGGCCA
MscI 1235 TGGCCA TGG^CCA
2727 TGGCCA
MslI 3096 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3598 CACAGGGATG
NarI 44 GGCGCC GG^CGCC
2994 GGCGCC
NcoI 60 CCATGG C^CATGG
4111 CCATGG
RcaI 385 TCATGA T^CATGA
513 TCATGA
SbfI 874 CCTGCAGG CCTGCA^GG
4664 CCTGCAGG
SfoI 44 GGCGCC GGC^GCC
2994 GGCGCC
Sse8387I 874 CCTGCAGG CCTGCA^GG
4664 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII