##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AflII AgeI AocI
AseI AsnI Asp700I AviII BamHI
BcgI BciVI BclI BfrI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI Eco81I EcoRI EcoRV
FbaI FseI FspI HindIII HpaI
MamI MfeI MluI MroI MunI
NdeI NheI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII ScaI SfuI SmaI SnaBI
SpeI SspI SwaI XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 5198 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2053 GGGCCC GGGCC^C
AscI 872 GGCGCGCC GG^CGCGCC
AspEI 5198 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 358 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3887 CACGTG CAC^GTG
BsmBI 135 CGTCTC CGTCTC(1/5)
BssSI 5682 CACGAG CACGAG(-5/-1)
DraIII 4787 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1342 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5198 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
NcoI 5156 CCATGG C^CATGG
NotI 598 GCGGCCGC GC^GGCCGC
NruI 1458 TCGCGA TCG^CGA
PflFI 358 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3887 CACGTG CAC^GTG
PmlI 3887 CACGTG CAC^GTG
SacI 1612 GAGCTC GAGCT^C
SalI 2002 GTCGAC G^TCGAC
SapI 3455 GAAGAGC GCTCTTC(1/4)
SbfI 4801 CCTGCAGG CCTGCA^GG
SexAI 5612 ACCTGGT A^CC[AT]GGT
SfiI 2764 GGCCATGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1976 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 4801 CCTGCAGG CCTGCA^GG
Tth111I 358 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 760 GACGTC GACGT^C
1918 GACGTC
Acc65I 3989 GGTACC G^GTACC
4686 GGTACC
AccI 538 GTCTAC GT^[AC][GT]AC
2002 GTCGAC
Alw44I 1069 GTGCAC G^TGCAC
4808 GTGCAC
Aor51HI 575 AGCGCT AGC^GCT
656 AGCGCT
ApaLI 1069 GTGCAC G^TGCAC
4808 GTGCAC
Asp718I 3989 GGTACC G^GTACC
4686 GGTACC
BbeI 283 GGCGCC GGCGC^C
460 GGCGCC
BbvCI 3007 CCTCAGC CCTCAGC(-5/-2)
3464 CCTCAGC
BglII 3768 AGATCT A^GATCT
5686 AGATCT
BsaAI 3887 CACGTG [CT]AC^GT[GA]
4787 CACGTA
BspHI 3773 TCATGA T^CATGA
4365 TCATGA
BsrBI 243 CCGCTC CCGCTC(-3/-3)
3525 GAGCGG
BsrDI 4936 GCAATG GCAATG(2/0)
5126 GCAATG
Eco47III 575 AGCGCT AGC^GCT
656 AGCGCT
EcoNI 141 CCTACGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2878 CCTCTCTGAGG
HincII 1081 GTCAAC GT[CT]^[GA]AC
2002 GTCGAC
HindII 1081 GTCAAC GT[CT]^[GA]AC
2002 GTCGAC
KasI 283 GGCGCC G^GCGCC
460 GGCGCC
KpnI 3989 GGTACC GGTAC^C
4686 GGTACC
NarI 283 GGCGCC GG^CGCC
460 GGCGCC
PaeR7I 571 CTCGAG C^TCGAG
4309 CTCGAG
RcaI 3773 TCATGA T^CATGA
4365 TCATGA
SfoI 283 GGCGCC GGC^GCC
460 GGCGCC
TfiI 3003 GATTC G^A[AT]TC
5630 GAATC
XhoI 571 CTCGAG C^TCGAG
4309 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrFI BsrGI BsrI BssHII BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII