##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AviII AvrII BanII
BbeI BbrPI BciVI BfrI BglII
BlnI BsaAI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BstBI BstUI Cfr10I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoRI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 255 AACGTT AA^CGTT
AocI 3281 CCTCAGG CC^T[ACGT]AGG
Asp700I 3446 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 51 GGATCC G^GATCC
BanI 3968 GGCACC G^G[CT][GA]CC
BbsI 1080 GAAGAC GAAGAC(2/6)
BcgI 4002 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1762 TGATCA T^GATCA
BglI 3908 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 3967 GGGCAC G[AGT]GC[ACT]^C
BpuAI 1080 GAAGAC GAAGAC(2/6)
BsaBI 169 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1286I 3967 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 1741 TGTACA T^GTACA
BspMI 3867 GCAGGT ACCTGC(4/8)
BsrDI 2090 CATTGC GCAATG(2/0)
BsrGI 1741 TGTACA T^GTACA
BssSI 1119 CACGAG CACGAG(-5/-1)
BstEII 1927 GGTAACC G^GT[ACGT]ACC
BstPI 1927 GGTAACC G^GT[ACGT]ACC
Bsu36I 3281 CCTCAGG CC^T[ACGT]AGG
CfoI 2209 GCGC GCG^C
Eco81I 3281 CCTCAGG CC^T[ACGT]AGG
EcoNI 3517 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1927 GGTAACC G^GT[ACGT]ACC
EcoRV 3246 GATATC GAT^ATC
EcoT22I 373 ATGCAT ATGCA^T
FauI 3965 GCGGG CCCGC(4/6)
FbaI 1762 TGATCA T^GATCA
HhaI 2209 GCGC GCG^C
HinP1I 2209 GCGC G^CGC
MamI 169 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 904 CAATTG C^AATTG
MunI 904 CAATTG C^AATTG
NsiI 373 ATGCAT ATGCA^T
Psp1406I 255 AACGTT AA^CGTT
PstI 463 CTGCAG CTGCA^G
ScaI 3848 AGTACT AGT^ACT
SphI 4061 GCATGC GCATG^C
SspBI 1741 TGTACA T^GTACA
StuI 316 AGGCCT AGG^CCT
XbaI 2052 TCTAGA T^CTAGA
XmnI 3446 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AseI 1872 ATTAAT AT^TAAT
2184 ATTAAT
AsnI 1872 ATTAAT AT^TAAT
2184 ATTAAT
AspI 2473 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4087 GACTCCGTC
AvaI 22 CCCGGG C^[CT]CG[GA]G
3298 CCCGGG
BbvCI 2382 GCTGAGG CCTCAGC(-5/-2)
3996 GCTGAGG
BlpI 185 GCTGAGC GC^T[ACGT]AGC
545 GCTGAGC
BpmI 2450 CTCCAG CTGGAG(16/14)
1363 CTGGAG
Bpu1102I 185 GCTGAGC GC^T[ACGT]AGC
545 GCTGAGC
BsaI 2283 GAGACC GGTCTC(1/5)
3896 GAGACC
BsgI 1418 CTGCAC GTGCAG(16/14)
2445 CTGCAC
BsmBI 2478 CGTCTC CGTCTC(1/5)
4092 CGTCTC
BsmI 371 GAATGC GAATGC(1/-1)
3511 GCATTC
BsoBI 22 CCCGGG C^[CT]CG[GA]G
3298 CCCGGG
BspHI 1406 TCATGA T^CATGA
1616 TCATGA
BstYI 51 GGATCC [GA]^GATC[CT]
808 AGATCC
CelII 185 GCTGAGC GC^T[ACGT]AGC
545 GCTGAGC
HincII 681 GTCAAC GT[CT]^[GA]AC
3220 GTTGAC
HindII 681 GTCAAC GT[CT]^[GA]AC
3220 GTTGAC
HindIII 2766 AAGCTT A^AGCTT
3779 AAGCTT
MflI 51 GGATCC [GA]^GATC[CT]
808 AGATCC
MspA1I 191 CAGCTG C[AC]G^C[GT]G
1014 CAGCTG
PflFI 2473 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4087 GACTCCGTC
PflMI 475 CCAACTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1070 CCATCAAGTGG
PshBI 1872 ATTAAT AT^TAAT
2184 ATTAAT
PvuII 191 CAGCTG CAG^CTG
1014 CAGCTG
RcaI 1406 TCATGA T^CATGA
1616 TCATGA
SmaI 22 CCCGGG CCC^GGG
3298 CCCGGG
SmlI 1209 CTTGAG C^T[CT][GA]AG
3358 CTTGAG
SpeI 478 ACTAGT A^CTAGT
1701 ACTAGT
Tth111I 2473 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4087 GACTCCGTC
Van91I 475 CCAACTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1070 CCATCAAGTGG
XhoII 51 GGATCC [GA]^GATC[CT]
808 AGATCC
XmaCI 22 CCCGGG C^CCGGG
3298 CCCGGG
XmaI 22 CCCGGG C^CCGGG
3298 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BalI
BbvI BcnI BfaI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstXI
BstZ17I Cac8I Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I