HUGE

Gene/Protein Characteristic Table for KIAA1165

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00753
Accession No. :	AB032991
Description :	NEDD4 family-interacting protein 2.
HUGO Gene Name :	Nedd4 family interacting protein 2 (NDFIP2)
Clone Name :	hk01631 [Vector Info]
Flexi ORF Clone :	pF1KSDA1165
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4415 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 3560 bp

Genome contig ID gi51511729f_78853496
PolyA signal sequence
(AATAAA,-29)
+----*----+----*----+----*----+----
AATTTTAATAAACAAAATATCATCTTTTTGAAAAT
Flanking genome sequence
(174717 - 174766)
----+----*----+----*----+----*----+----*----+----*
AATTTATGTTCATTTCTACTTTGTAAAGTATCATTGGTCACCTACGTCAG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 13 f 78953496 79028211 8 99.2 Perfect prediction

Features of the protein sequence	Description

Length: 284 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAC77372	1.7e-100	100.0	putative NFkB a...	Homo sapiens
Q9NV92	3.2e-100	99.6	NEDD4 family-in...	Homo sapiens
CAH93486	5.2e-99	98.9	hypothetical pr...	Pongo abelii
XP_522688	7.4e-99	98.9	similar to puta...	Pan troglodytes
XP_001092556	9.5e-97	97.2	Nedd4 family in...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

None - - - - -

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 179 GIFMLAFFMAFIFNWLGFCLSFC 201 PRIMARY 23

2 210 YGAICGFGLSLIKWILIVRFSDY 232 PRIMARY 23

3 239 GQYWLWWIFLVLGLLLFFRGFVN 261 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	179	GIFMLAFFMAFIFNWLGFCLSFC	201	PRIMARY	23
2	210	YGAICGFGLSLIKWILIVRFSDY	232	PRIMARY	23
3	239	GQYWLWWIFLVLGLLLFFRGFVN	261	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: AGATGTGGAAGTGTGAACCTG

Primer_r
: GCAACCCCAACCTAATTTCAC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 13

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: AGATGTGGAAGTGTGAACCTG
Primer_r	: GCAACCCCAACCTAATTTCAC
PCR product length	: 152 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	3560 bp
Genome contig ID	gi51511729f_78853496
PolyA signal sequence (AATAAA,-29)	+--------+--------+----*----+---- AATTTTAATAAACAAAATATCATCTTTTTGAAAAT
Flanking genome sequence (174717 - 174766)	----+--------+--------+--------+--------+----* AATTTATGTTCATTTCTACTTTGTAAAGTATCATTGGTCACCTACGTCAG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	13	f	78953496	79028211	8	99.2	Perfect prediction

Gene/Protein Characteristic Table for KIAA1165

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information