##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BbeI BbrPI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BspDI BspHI BsrBI
BsrFI BssHII BssSI BstBI BstEII
BstPI BstUI CelII Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI FauI
FbaI FseI HaeII HgaI Hin1I
HpaI KasI KpnI KspI MamI
MluI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Van91I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 9 TCCGGA T^CCGGA
AflIII 474 ACATGT A^C[GA][CT]GT
ApaI 2657 GGGCCC GGGCC^C
AspHI 2862 GAGCAC G[AT]GC[AT]^C
AspI 848 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 128 CTCGGG C^[CT]CG[GA]G
AviII 488 TGCGCA TGC^GCA
BalI 3871 TGGCCA TGG^CCA
BanI 284 GGCACC G^G[CT][GA]CC
BcgI 1542 GCAGGATACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 4042 GGTCTC GGTCTC(1/5)
BseAI 9 TCCGGA T^CCGGA
BsgI 3894 GTGCAG GTGCAG(16/14)
BsiHKAI 2862 GAGCAC G[AT]GC[AT]^C
BsoBI 128 CTCGGG C^[CT]CG[GA]G
Bsp1407I 119 TGTACA T^GTACA
BspEI 9 TCCGGA T^CCGGA
BspLU11I 474 ACATGT A^CATGT
BsrGI 119 TGTACA T^GTACA
BstAPI 1504 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 489 GCGC GCG^C
DraI 816 TTTAAA TTT^AAA
EcoNI 1030 CCTGGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 901 GATATC GAT^ATC
FspI 488 TGCGCA TGC^GCA
HhaI 489 GCGC GCG^C
HinP1I 489 GCGC G^CGC
HincII 2414 GTTGAC GT[CT]^[GA]AC
HindII 2414 GTTGAC GT[CT]^[GA]AC
MaeII 134 ACGT A^CGT
MfeI 2143 CAATTG C^AATTG
MluNI 3871 TGGCCA TGG^CCA
MroI 9 TCCGGA T^CCGGA
MscI 3871 TGGCCA TGG^CCA
MunI 2143 CAATTG C^AATTG
NheI 1192 GCTAGC G^CTAGC
PflFI 848 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 1029 ACCTGGT A^CC[AT]GGT
SphI 2295 GCATGC GCATG^C
SspBI 119 TGTACA T^GTACA
TaqI 3990 TCGA T^CGA
Tth111I 848 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3990 TCGA T^CGA
XcmI 3365 CCAGAGGTAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BbvCI 3851 CCTCAGC CCTCAGC(-5/-2)
3623 GCTGAGG
BcnI 68 CCCGG CC^[GC]GG
2812 CCCGG
BpmI 3174 CTGGAG CTGGAG(16/14)
3845 CTGGAG
BsaWI 9 TCCGGA [AT]^CCGG[AT]
2767 ACCGGA
BsmI 1802 GAATGC GAATGC(1/-1)
2771 GAATGC
BsrDI 1387 CATTGC GCAATG(2/0)
4096 CATTGC
Bst1107I 638 GTATAC GTA^TAC
2526 GTATAC
BstXI 3309 CCAACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3701 CCATTACTCTGG
BstZ17I 638 GTATAC GTA^TAC
2526 GTATAC
DraIII 710 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4102 CACAGTGTG
EcoT22I 2258 ATGCAT ATGCA^T
2293 ATGCAT
HindIII 541 AAGCTT A^AGCTT
2871 AAGCTT
MspA1I 1499 CCGCTG C[AC]G^C[GT]G
1508 CCGCTG
NciI 68 CCCGG CC^[GC]GG
2812 CCCGG
NcoI 1638 CCATGG C^CATGG
2583 CCATGG
NsiI 2258 ATGCAT ATGCA^T
2293 ATGCAT
PshAI 2900 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3284 GACAATAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI AocI ApoI
AvaII BanII BbsI BbvI BciVI
BfaI BfrI BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XbaI XhoII