##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AflII AgeI Aor51HI
AscI Asp718I AviII BbeI BbrPI
BfrI BsaAI BsaBI BsiWI BsmBI
BspDI BssHII BssSI Bst1107I BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I FseI FspI HindIII
KasI KpnI MamI MluI NaeI
NarI NgoMIV NheI NotI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SalI ScaI
SfiI SfoI SgrAI SnaBI SpeI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 3285 TCCGGA T^CCGGA
AclI 2325 AACGTT AA^CGTT
AhdI 1908 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1078 GTGCAC G^TGCAC
ApaLI 1078 GTGCAC G^TGCAC
AseI 947 ATTAAT AT^TAAT
AsnI 947 ATTAAT AT^TAAT
Asp700I 131 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1908 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4058 CCTAGG C^CTAGG
BciVI 2368 GTATCC GTATCC(6/5)
BlnI 4058 CCTAGG C^CTAGG
BsaI 1037 GAGACC GGTCTC(1/5)
BseAI 3285 TCCGGA T^CCGGA
BsiEI 3766 CGACCG CG[GA][CT]^CG
BspEI 3285 TCCGGA T^CCGGA
BspHI 444 TCATGA T^CATGA
BspLU11I 576 ACATGT A^CATGT
BstBI 1617 TTCGAA TT^CGAA
BstXI 711 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2195 TTTAAA TTT^AAA
Eam1105I 1908 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2024 GAATTC G^AATTC
EcoRV 1706 GATATC GAT^ATC
HpaI 2038 GTTAAC GTT^AAC
KspI 1368 CCGCGG CCGC^GG
MfeI 2433 CAATTG C^AATTG
MroI 3285 TCCGGA T^CCGGA
MunI 2433 CAATTG C^AATTG
NspV 1617 TTCGAA TT^CGAA
PshBI 947 ATTAAT AT^TAAT
Psp1406I 2325 AACGTT AA^CGTT
RcaI 444 TCATGA T^CATGA
SacII 1368 CCGCGG CCGC^GG
SexAI 3324 ACCTGGT A^CC[AT]GGT
SfuI 1617 TTCGAA TT^CGAA
SmaI 348 CCCGGG CCC^GGG
SphI 4075 GCATGC GCATG^C
XcmI 2532 CCATTGTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 348 CCCGGG C^CCGGG
XmaI 348 CCCGGG C^CCGGG
XmnI 131 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 2258 GTCTAC GT^[AC][GT]AC
3254 GTCTAC
AcyI 2354 GGCGTC G[GA]^CG[CT]C
3500 GGCGTC
ApaI 7 GGGCCC GGGCC^C
3481 GGGCCC
AspI 530 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
797 GACATTGTC
AvaI 348 CCCGGG C^[CT]CG[GA]G
1765 CCCGAG
BbvCI 3356 CCTCAGC CCTCAGC(-5/-2)
2228 GCTGAGG
BcgI 1805 CGAAGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
468 GCAAGGCCATCG
BclI 1446 TGATCA T^GATCA
2248 TGATCA
BglI 1558 GCCCCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1803 GCCGAAGAGGC
BlpI 3596 GCTGAGC GC^T[ACGT]AGC
3912 GCTAAGC
Bpu1102I 3596 GCTGAGC GC^T[ACGT]AGC
3912 GCTAAGC
BsaHI 2354 GGCGTC G[GA]^CG[CT]C
3500 GGCGTC
BsaWI 3285 TCCGGA [AT]^CCGG[AT]
3768 ACCGGA
BsmI 226 GAATGC GAATGC(1/-1)
3447 GCATTC
BsoBI 348 CCCGGG C^[CT]CG[GA]G
1765 CCCGAG
BsrFI 2565 ACCGGC [GA]^CCGG[CT]
3534 ACCGGC
BstAPI 985 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2575 GCATCGGCTGC
BstEII 433 GGTCACC G^GT[ACGT]ACC
3383 GGTGACC
BstPI 433 GGTCACC G^GT[ACGT]ACC
3383 GGTGACC
CelII 3596 GCTGAGC GC^T[ACGT]AGC
3912 GCTAAGC
Cfr10I 2565 ACCGGC [GA]^CCGG[CT]
3534 ACCGGC
DraIII 739 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2639 CACAATGTG
EcoO65I 433 GGTCACC G^GT[ACGT]ACC
3383 GGTGACC
EcoT22I 160 ATGCAT ATGCA^T
228 ATGCAT
FbaI 1446 TGATCA T^GATCA
2248 TGATCA
Hin1I 2354 GGCGTC G[GA]^CG[CT]C
3500 GGCGTC
HincII 803 GTCAAC GT[CT]^[GA]AC
2038 GTTAAC
HindII 803 GTCAAC GT[CT]^[GA]AC
2038 GTTAAC
NcoI 2043 CCATGG C^CATGG
2298 CCATGG
NdeI 860 CATATG CA^TATG
2594 CATATG
NruI 1237 TCGCGA TCG^CGA
3376 TCGCGA
NsiI 160 ATGCAT ATGCA^T
228 ATGCAT
PflFI 530 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
797 GACATTGTC
SbfI 1456 CCTGCAGG CCTGCA^GG
3460 CCTGCAGG
SmlI 869 CTCAAG C^T[CT][GA]AG
1672 CTTGAG
Sse8387I 1456 CCTGCAGG CCTGCA^GG
3460 CCTGCAGG
Tth111I 530 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
797 GACATTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrBI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII