##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AcsI AflII AgeI AhdI
ApoI AscI AspEI AspI AviII
AvrII BciVI BfrI BlnI BsiEI
BsiWI BspDI BspLU11I Bst1107I BstBI
BstZ17I ClaI DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoT22I FseI
FspI HindIII KspI MluI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PvuI
SacII SbfI SfuI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 3831 GGTACC G^GTACC
AccI 3022 GTCGAC GT^[AC][GT]AC
AccIII 1794 TCCGGA T^CCGGA
AclI 3584 AACGTT AA^CGTT
AocI 257 CCTGAGG CC^T[ACGT]AGG
AseI 1581 ATTAAT AT^TAAT
AsnI 1581 ATTAAT AT^TAAT
Asp700I 1685 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3831 GGTACC G^GTACC
BbeI 90 GGCGCC GGCGC^C
BclI 1009 TGATCA T^GATCA
BglII 952 AGATCT A^GATCT
BlpI 1460 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1460 GCTCAGC GC^T[ACGT]AGC
BsaBI 2315 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1794 TCCGGA [AT]^CCGG[AT]
BseAI 1794 TCCGGA T^CCGGA
BsmI 4040 GCATTC GAATGC(1/-1)
BspEI 1794 TCCGGA T^CCGGA
BsrDI 5141 GCAATG GCAATG(2/0)
BssHII 126 GCGCGC G^CGCGC
BstAPI 4021 GCAAGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 878 GGTGACC G^GT[ACGT]ACC
BstPI 878 GGTGACC G^GT[ACGT]ACC
Bsu36I 257 CCTGAGG CC^T[ACGT]AGG
CelII 1460 GCTCAGC GC^T[ACGT]AGC
CpoI 131 CGGTCCG CG^G[AT]CCG
DraI 4305 TTTAAA TTT^AAA
Eco81I 257 CCTGAGG CC^T[ACGT]AGG
EcoO65I 878 GGTGACC G^GT[ACGT]ACC
FbaI 1009 TGATCA T^GATCA
KasI 90 GGCGCC G^GCGCC
KpnI 3831 GGTACC GGTAC^C
MamI 2315 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4482 CAATTG C^AATTG
MroI 1794 TCCGGA T^CCGGA
MunI 4482 CAATTG C^AATTG
NarI 90 GGCGCC GG^CGCC
PaeR7I 2340 CTCGAG C^TCGAG
PshBI 1581 ATTAAT AT^TAAT
Psp1406I 3584 AACGTT AA^CGTT
RsrII 131 CGGTCCG CG^G[AT]CCG
SalI 3022 GTCGAC G^TCGAC
ScaI 1783 AGTACT AGT^ACT
SfiI 3310 GGCCCATGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 90 GGCGCC GGC^GCC
SgrAI 92 CGCCGGCG C[GA]^CCGG[CT]G
XhoI 2340 CTCGAG C^TCGAG
XmnI 1685 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 3633 ACACGT A^C[GA][CT]GT
3635 ACGTGT
BamHI 1651 GGATCC G^GATCC
3015 GGATCC
BbrPI 1701 CACGTG CAC^GTG
3634 CACGTG
BbvCI 5063 CCTCAGC CCTCAGC(-5/-2)
1910 GCTGAGG
BcgI 1490 CGAAGTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
59 GCAACAGCTTCG
BsmBI 2749 CGTCTC CGTCTC(1/5)
3670 GAGACG
Bsp1407I 2811 TGTACA T^GTACA
5158 TGTACA
BsrBI 309 CCGCTC CCGCTC(-3/-3)
3765 GAGCGG
BsrGI 2811 TGTACA T^GTACA
5158 TGTACA
BssSI 2078 CACGAG CACGAG(-5/-1)
1656 CTCGTG
DraIII 2115 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
3521 CACTTCGTG
EcoRV 247 GATATC GAT^ATC
1482 GATATC
HpaI 3593 GTTAAC GTT^AAC
3802 GTTAAC
NspI 1310 GCATGC [GA]CATG^[CT]
2630 GCATGC
PflMI 2114 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3520 CCACTTCGTGG
PmaCI 1701 CACGTG CAC^GTG
3634 CACGTG
PmlI 1701 CACGTG CAC^GTG
3634 CACGTG
PshAI 797 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4817 GACGGGAGTC
PstI 3890 CTGCAG CTGCA^G
3980 CTGCAG
SmaI 4360 CCCGGG CCC^GGG
4642 CCCGGG
SmlI 2340 CTCGAG C^T[CT][GA]AG
2412 CTTGAG
SphI 1310 GCATGC GCATG^C
2630 GCATGC
SspBI 2811 TGTACA T^GTACA
5158 TGTACA
TfiI 1439 GAATC G^A[AT]TC
4354 GATTC
Van91I 2114 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3520 CCACTTCGTGG
XmaCI 4360 CCCGGG C^CCGGG
4642 CCCGGG
XmaI 4360 CCCGGG C^CCGGG
4642 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII