HUGE

Gene/Protein Characteristic Table for KIAA1176

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK06843
Accession No. :	AB033002
Description :	Solute carrier family 12 member 5.
HUGO Gene Name :	solute carrier family 12 (potassium-chloride transporter), member 5 (SLC12A5)
Clone Name :	hg02851a [Vector Info]
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 3305 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 0 bp

Genome contig ID gi51511747f_43996993
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
TGGACCGGGTGATGCTGGTCCGCGGCGGCGGCCGC
Flanking genome sequence
(122636 - 122685)
----+----*----+----*----+----*----+----*----+----*
GAGGTCATCACCATCTACTCCTGAGAACCAGGACCTGCCACCCGGGCCCG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 20 f 44091413 44119627 25 99.5 Perfect prediction

Features of the protein sequence	Description

Length: 1101 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
CAH74054	99.9	solute carrier ...	Homo sapiens
AAG43493	99.9	electroneutral ...	Homo sapiens
BAF82447	99.8	unnamed protein...	Homo sapiens
XP_001104798	99.3	similar to solu...	Macaca mulatta
EAW75773	99.5	solute carrier ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR000076 15 21 PR01081 K-Cl co-transporter

IPR000076 35 43 PR01081 K-Cl co-transporter

IPR000076 236 248 PR01081 K-Cl co-transporter

IPR000076 271 288 PR01081 K-Cl co-transporter

IPR000076 512 528 PR01081 K-Cl co-transporter

IPR000076 538 546 PR01081 K-Cl co-transporter

HMMPfam IPR004841 94 668 PF00324 Amino acid permease-associated region

HMMTigr IPR004842 13 1101 TIGR00930 Na-K-Cl cotransporter superfamily

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000076	15	21	PR01081	K-Cl co-transporter
IPR000076	35	43	PR01081	K-Cl co-transporter
IPR000076	236	248	PR01081	K-Cl co-transporter
IPR000076	271	288	PR01081	K-Cl co-transporter
IPR000076	512	528	PR01081	K-Cl co-transporter
IPR000076	538	546	PR01081	K-Cl co-transporter
HMMPfam	IPR004841	94	668	PF00324	Amino acid permease-associated region
HMMTigr	IPR004842	13	1101	TIGR00930	Na-K-Cl cotransporter superfamily

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 89 MGTFMGVYLPCLQNIFGVILFLR 111 PRIMARY 23

2 123 ESFCMVFICCSCTMLTAISMSAI 145 PRIMARY 23

3 166 PEFGGAVGLCFYLGTTFAGAMYI 188 SECONDARY 23

4 193 EILLAYLFPAMAIFKAEDASGEA 215 SECONDARY 23

5 224 VYGTCVLTCMATVVFVGVKYVNK 246 PRIMARY 23

6 250 VFLGCVILSILAIYAGVIKSAFD 272 PRIMARY 23

7 389 TSYFTLLVGIYFPSVTGIMAGSN 411 SECONDARY 23

8 428 ILAIATTSAVYISSVVLFGACIE 450 PRIMARY 23

9 465 NLVVGTLAWPSPWVIVIGSFFST 487 SECONDARY 23

10 528 WALLLTACICEIGILIASLDEVA 550 PRIMARY 23

11 553 LSMFFLMCYMFVNLACAVQTLLR 575 PRIMARY 23

12 595 MSLCLALMFICSWYYALVAMLIA 617 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	89	MGTFMGVYLPCLQNIFGVILFLR	111	PRIMARY	23
2	123	ESFCMVFICCSCTMLTAISMSAI	145	PRIMARY	23
3	166	PEFGGAVGLCFYLGTTFAGAMYI	188	SECONDARY	23
4	193	EILLAYLFPAMAIFKAEDASGEA	215	SECONDARY	23
5	224	VYGTCVLTCMATVVFVGVKYVNK	246	PRIMARY	23
6	250	VFLGCVILSILAIYAGVIKSAFD	272	PRIMARY	23
7	389	TSYFTLLVGIYFPSVTGIMAGSN	411	SECONDARY	23
8	428	ILAIATTSAVYISSVVLFGACIE	450	PRIMARY	23
9	465	NLVVGTLAWPSPWVIVIGSFFST	487	SECONDARY	23
10	528	WALLLTACICEIGILIASLDEVA	550	PRIMARY	23
11	553	LSMFFLMCYMFVNLACAVQTLLR	575	PRIMARY	23
12	595	MSLCLALMFICSWYYALVAMLIA	617	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: GCAACCCCAAGGAAAGCAGTC

Primer_r
: TTCATGCTCCCTACTTCCCTG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 20

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: GCAACCCCAAGGAAAGCAGTC
Primer_r	: AAGGAGGACACCATAGGGCTG
PCR product length	: 124 bp
PCR conditions	: 95 °C15 sec66 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	0 bp
Genome contig ID	gi51511747f_43996993
PolyA signal sequence (None)	+--------+--------+----*----+---- TGGACCGGGTGATGCTGGTCCGCGGCGGCGGCCGC
Flanking genome sequence (122636 - 122685)	----+--------+--------+--------+--------+----* GAGGTCATCACCATCTACTCCTGAGAACCAGGACCTGCCACCCGGGCCCG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	20	f	44091413	44119627	25	99.5	Perfect prediction

Gene/Protein Characteristic Table for KIAA1176

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information