##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbrPI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsiWI
BsmI BspDI BspHI BssHII Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EcoRI
EcoRV FbaI FseI FspI HpaI
KpnI MaeI MluI NaeI NdeI
NgoMIV NheI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspI
StuI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1311 GTCTAC GT^[AC][GT]AC
AclI 2406 AACGTT AA^CGTT
AflIII 1684 ACATGT A^C[GA][CT]GT
Aor51HI 2434 AGCGCT AGC^GCT
Asp700I 2192 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1446 GGATCC G^GATCC
BbvCI 1721 GCTGAGG CCTCAGC(-5/-2)
BciVI 1909 GGATAC GTATCC(6/5)
BsaI 487 GGTCTC GGTCTC(1/5)
Bsp1407I 559 TGTACA T^GTACA
BspLU11I 1684 ACATGT A^CATGT
BsrFI 248 GCCGGT [GA]^CCGG[CT]
BsrGI 559 TGTACA T^GTACA
Cfr10I 248 GCCGGT [GA]^CCGG[CT]
Eco47III 2434 AGCGCT AGC^GCT
HincII 732 GTCAAC GT[CT]^[GA]AC
HindII 732 GTCAAC GT[CT]^[GA]AC
HindIII 3180 AAGCTT A^AGCTT
KspI 3290 CCGCGG CCGC^GG
MfeI 433 CAATTG C^AATTG
MseI 2739 TTAA T^TAA
MunI 433 CAATTG C^AATTG
NotI 3298 GCGGCCGC GC^GGCCGC
Psp1406I 2406 AACGTT AA^CGTT
SacI 1055 GAGCTC GAGCT^C
SacII 3290 CCGCGG CCGC^GG
SbfI 1541 CCTGCAGG CCTGCA^GG
Sse8387I 1541 CCTGCAGG CCTGCA^GG
SspBI 559 TGTACA T^GTACA
Tru9I 2739 TTAA T^TAA
XmnI 2192 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccII 2824 CGCG CG^CG
3291 CGCG
AccIII 2800 TCCGGA T^CCGGA
2978 TCCGGA
Alw44I 2037 GTGCAC G^TGCAC
2472 GTGCAC
AlwNI 1713 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
1995 CAGCTGCTG
AocI 1247 CCTGAGG CC^T[ACGT]AGG
2955 CCTGAGG
ApaLI 2037 GTGCAC G^TGCAC
2472 GTGCAC
BbeI 2167 GGCGCC GGCGC^C
2314 GGCGCC
BcgI 2903 CGATCAGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1123 GCACTCCTATCG
BglI 885 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2142 GCCCAGCGGGC
BsaBI 1616 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2543 GATGCGTATC
BsaWI 2800 TCCGGA [AT]^CCGG[AT]
2978 TCCGGA
BseAI 2800 TCCGGA T^CCGGA
2978 TCCGGA
BsiEI 3133 CGGCCG CG[GA][CT]^CG
3299 CGGCCG
BsmBI 909 GAGACG CGTCTC(1/5)
2850 GAGACG
BspEI 2800 TCCGGA T^CCGGA
2978 TCCGGA
BssSI 2788 CACGAG CACGAG(-5/-1)
1398 CTCGTG
BstAPI 624 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1712 GCAGACGCTGC
BstUI 2824 CGCG CG^CG
3291 CGCG
Bsu36I 1247 CCTGAGG CC^T[ACGT]AGG
2955 CCTGAGG
EagI 3133 CGGCCG C^GGCCG
3299 CGGCCG
EclXI 3133 CGGCCG C^GGCCG
3299 CGGCCG
Eco52I 3133 CGGCCG C^GGCCG
3299 CGGCCG
Eco81I 1247 CCTGAGG CC^T[ACGT]AGG
2955 CCTGAGG
EcoT22I 2655 ATGCAT ATGCA^T
2733 ATGCAT
KasI 2167 GGCGCC G^GCGCC
2314 GGCGCC
MamI 1616 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2543 GATGCGTATC
MroI 2800 TCCGGA T^CCGGA
2978 TCCGGA
MvnI 2824 CGCG CG^CG
3291 CGCG
NarI 2167 GGCGCC GG^CGCC
2314 GGCGCC
NsiI 2655 ATGCAT ATGCA^T
2733 ATGCAT
PaeR7I 1517 CTCGAG C^TCGAG
3249 CTCGAG
PpuMI 1243 GGGACCT [GA]G^G[AT]CC[CT]
3253 AGGTCCT
SfoI 2167 GGCGCC GGC^GCC
2314 GGCGCC
SmaI 26 CCCGGG CCC^GGG
3169 CCCGGG
SphI 2485 GCATGC GCATG^C
3124 GCATGC
TfiI 1694 GAATC G^A[AT]TC
2810 GAATC
Tsp509I 434 AATT ^AATT
1436 AATT
XcmI 556 CCATGTACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1007 CCAGGGCATCCCTGG
XhoI 1517 CTCGAG C^TCGAG
3249 CTCGAG
XmaCI 26 CCCGGG C^CCGGG
3169 CCCGGG
XmaI 26 CCCGGG C^CCGGG
3169 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XhoII