##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BclI BfrI BlnI BlpI
Bpu1102I BsmBI BsmI BspDI BspHI
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eam1105I EarI
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI Ksp632I MfeI MluI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Alw44I 771 GTGCAC G^TGCAC
ApaLI 771 GTGCAC G^TGCAC
BalI 301 TGGCCA TGG^CCA
BbrPI 768 CACGTG CAC^GTG
BbsI 2262 GAAGAC GAAGAC(2/6)
BbvCI 2043 GCTGAGG CCTCAGC(-5/-2)
BcgI 131 GCAGCACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1876 GTATCC GTATCC(6/5)
BfaI 42 CTAG C^TAG
BpuAI 2262 GAAGAC GAAGAC(2/6)
BsaBI 1259 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 1321 CGTACG C^GTACG
BspLU11I 1591 ACATGT A^CATGT
BsrBI 1422 GAGCGG CCGCTC(-3/-3)
BssHII 2069 GCGCGC G^CGCGC
BstEII 1634 GGTCACC G^GT[ACGT]ACC
BstPI 1634 GGTCACC G^GT[ACGT]ACC
EagI 1618 CGGCCG C^GGCCG
EclXI 1618 CGGCCG C^GGCCG
Eco52I 1618 CGGCCG C^GGCCG
EcoO65I 1634 GGTCACC G^GT[ACGT]ACC
MaeI 42 CTAG C^TAG
MamI 1259 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 2262 GAAGA GAAGA(8/7)
MluNI 301 TGGCCA TGG^CCA
MscI 301 TGGCCA TGG^CCA
NcoI 1537 CCATGG C^CATGG
PflMI 293 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 227 GAGTC GAGTC(4/5)
PmaCI 768 CACGTG CAC^GTG
PmlI 768 CACGTG CAC^GTG
PpuMI 1432 GGGACCT [GA]G^G[AT]CC[CT]
SacI 1029 GAGCTC GAGCT^C
SfcI 1763 CTACAG C^T[GA][CT]AG
SphI 1532 GCATGC GCATG^C
StuI 1054 AGGCCT AGG^CCT
TfiI 392 GAATC G^A[AT]TC
Van91I 293 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1558 CCACCAGGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 395 TCCGGA T^CCGGA
550 TCCGGA
AcsI 142 GAATTT [GA]^AATT[CT]
1389 AAATTC
AcyI 423 GACGCC G[GA]^CG[CT]C
2103 GACGCC
AlwI 2084 GATCC GGATC(4/5)
2156 GATCC
ApoI 142 GAATTT [GA]^AATT[CT]
1389 AAATTC
BanI 1194 GGCACC G^G[CT][GA]CC
1962 GGCACC
BglII 1624 AGATCT A^GATCT
1780 AGATCT
BsaAI 768 CACGTG [CT]AC^GT[GA]
1898 CACGTA
BsaHI 423 GACGCC G[GA]^CG[CT]C
2103 GACGCC
BsaI 636 GAGACC GGTCTC(1/5)
1943 GGTCTC
BsaWI 395 TCCGGA [AT]^CCGG[AT]
550 TCCGGA
BseAI 395 TCCGGA T^CCGGA
550 TCCGGA
Bsp1407I 1141 TGTACA T^GTACA
2323 TGTACA
BspEI 395 TCCGGA T^CCGGA
550 TCCGGA
BsrDI 1394 CATTGC GCAATG(2/0)
1846 GCAATG
BsrGI 1141 TGTACA T^GTACA
2323 TGTACA
BstAPI 20 GCACAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2225 GCAGAGCGTGC
BstXI 1276 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1561 CCAGGGCCGTGG
DraIII 765 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
887 CACTCTGTG
DrdI 1827 GACTTTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2265 GACTGACCCGTC
EcoNI 797 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
953 CCTGGAGAAGG
Hin1I 423 GACGCC G[GA]^CG[CT]C
2103 GACGCC
HincII 1914 GTCAAC GT[CT]^[GA]AC
2142 GTCAAC
HindII 1914 GTCAAC GT[CT]^[GA]AC
2142 GTCAAC
HinfI 227 GAGTC G^A[ACGT]TC
392 GAATC
KspI 434 CCGCGG CCGC^GG
1646 CCGCGG
MroI 395 TCCGGA T^CCGGA
550 TCCGGA
NaeI 339 GCCGGC GCC^GGC
718 GCCGGC
NgoMIV 339 GCCGGC G^CCGGC
718 GCCGGC
NspI 1532 GCATGC [GA]CATG^[CT]
1591 ACATGT
SacII 434 CCGCGG CCGC^GG
1646 CCGCGG
ScaI 1105 AGTACT AGT^ACT
1297 AGTACT
SspBI 1141 TGTACA T^GTACA
2323 TGTACA
Tsp509I 143 AATT ^AATT
1390 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI AspHI AvaI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SmaI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII XmaCI
XmaI