##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI BcgI BclI
BfrI BglII BsaBI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MspA1I
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 168 CGCG CG^CG
AccIII 1959 TCCGGA T^CCGGA
AflIII 96 ACATGT A^C[GA][CT]GT
AlwNI 1288 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 946 CCTAAGG CC^T[ACGT]AGG
AspHI 694 GTGCTC G[AT]GC[AT]^C
AviII 2371 TGCGCA TGC^GCA
AvrII 58 CCTAGG C^CTAGG
BamHI 1717 GGATCC G^GATCC
BbrPI 2129 CACGTG CAC^GTG
BciVI 630 GTATCC GTATCC(6/5)
BglI 1180 GCCTTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 58 CCTAGG C^CTAGG
BlpI 1644 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1644 GCTGAGC GC^T[ACGT]AGC
BsaAI 2129 CACGTG [CT]AC^GT[GA]
BseAI 1959 TCCGGA T^CCGGA
BsiHKAI 694 GTGCTC G[AT]GC[AT]^C
BspEI 1959 TCCGGA T^CCGGA
BspLU11I 96 ACATGT A^CATGT
BspMI 1973 GCAGGT ACCTGC(4/8)
BsrDI 1374 CATTGC GCAATG(2/0)
BsrFI 1903 GCCGGT [GA]^CCGG[CT]
BssSI 2361 CACGAG CACGAG(-5/-1)
BstEII 1781 GGTCACC G^GT[ACGT]ACC
BstPI 1781 GGTCACC G^GT[ACGT]ACC
BstUI 168 CGCG CG^CG
BstXI 390 CCAGTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 946 CCTAAGG CC^T[ACGT]AGG
CelII 1644 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1903 GCCGGT [GA]^CCGG[CT]
DraI 1874 TTTAAA TTT^AAA
EarI 1893 GAAGAG CTCTTC(1/4)
Eco81I 946 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1781 GGTCACC G^GT[ACGT]ACC
EcoRI 1283 GAATTC G^AATTC
FspI 2371 TGCGCA TGC^GCA
HindIII 2387 AAGCTT A^AGCTT
Ksp632I 1893 GAAGAG CTCTTC(1/4)
MroI 1959 TCCGGA T^CCGGA
MseI 1875 TTAA T^TAA
MvnI 168 CGCG CG^CG
NcoI 1585 CCATGG C^CATGG
PmaCI 2129 CACGTG CAC^GTG
PmlI 2129 CACGTG CAC^GTG
PstI 1703 CTGCAG CTGCA^G
ScaI 591 AGTACT AGT^ACT
SfiI 1179 GGCCTTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I 1875 TTAA T^TAA
XbaI 2551 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 1283 GAATTC [GA]^AATT[CT]
1829 GAATTT
ApoI 1283 GAATTC [GA]^AATT[CT]
1829 GAATTT
BalI 536 TGGCCA TGG^CCA
1038 TGGCCA
BbsI 832 GAAGAC GAAGAC(2/6)
967 GTCTTC
BbvCI 104 GCTGAGG CCTCAGC(-5/-2)
1988 GCTGAGG
BcnI 289 CCCGG CC^[GC]GG
2256 CCCGG
BpuAI 832 GAAGAC GAAGAC(2/6)
967 GTCTTC
BsaI 131 GAGACC GGTCTC(1/5)
2024 GGTCTC
DraIII 432 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1216 CACTATGTG
EaeI 536 TGGCCA [CT]^GGCC[GA]
1038 TGGCCA
FauI 169 GCGGG CCCGC(4/6)
1605 GCGGG
HgaI 31 GCGTC GACGC(5/10)
1939 GCGTC
MluNI 536 TGGCCA TGG^CCA
1038 TGGCCA
MscI 536 TGGCCA TGG^CCA
1038 TGGCCA
MslI 1650 CATGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1791 CACTCTTGTG
NciI 289 CCCGG CC^[GC]GG
2256 CCCGG
NspI 96 ACATGT [GA]CATG^[CT]
1649 GCATGT
PflMI 1506 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2216 CCAGGACGTGG
SmlI 891 CTCAAG C^T[CT][GA]AG
1244 CTTGAG
StuI 1897 AGGCCT AGG^CCT
2414 AGGCCT
TaqI 718 TCGA T^CGA
828 TCGA
TthHB8I 718 TCGA T^CGA
828 TCGA
Van91I 1506 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2216 CCAGGACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
ApaI AvaII BanI BanII BbeI
BbvI BfaI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NarI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TfiI TseI Tsp45I Tsp509I
TspRI XcmI XhoII