##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AvrII
BamHI BcgI BclI BfrI BlnI
BsaBI BseAI BsiEI BsiWI BsmBI
BspEI BsrFI BssHII Bst1107I BstEII
BstPI BstZ17I Bsu36I Cfr10I CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FbaI FseI HindIII HpaI KpnI
KspI MamI MluI MroI NaeI
NdeI NgoMIV NotI NruI PacI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SapI ScaI
SgrAI SnaBI SpeI SphI SspI
SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2740 GTAGAC GT^[AC][GT]AC
AccII 2651 CGCG CG^CG
AcyI 3025 GGCGCC G[GA]^CG[CT]C
ApaI 1697 GGGCCC GGGCC^C
AseI 643 ATTAAT AT^TAAT
AsnI 643 ATTAAT AT^TAAT
AviII 1760 TGCGCA TGC^GCA
BbeI 3025 GGCGCC GGCGC^C
BbrPI 1288 CACGTG CAC^GTG
BbsI 2188 GAAGAC GAAGAC(2/6)
BpuAI 2188 GAAGAC GAAGAC(2/6)
BsaAI 1288 CACGTG [CT]AC^GT[GA]
BsaHI 3025 GGCGCC G[GA]^CG[CT]C
BspDI 2116 ATCGAT AT^CGAT
BspHI 536 TCATGA T^CATGA
BsrBI 1816 GAGCGG CCGCTC(-3/-3)
BsrDI 2766 CATTGC GCAATG(2/0)
BssSI 772 CTCGTG CACGAG(-5/-1)
BstBI 1495 TTCGAA TT^CGAA
BstUI 2651 CGCG CG^CG
ClaI 2116 ATCGAT AT^CGAT
DraIII 610 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1513 GAATTC G^AATTC
FspI 1760 TGCGCA TGC^GCA
HaeII 3025 GGCGCC [GA]GCGC^[CT]
Hin1I 3025 GGCGCC G[GA]^CG[CT]C
HincII 1372 GTTGAC GT[CT]^[GA]AC
HindII 1372 GTTGAC GT[CT]^[GA]AC
KasI 3025 GGCGCC G^GCGCC
MvnI 2651 CGCG CG^CG
NarI 3025 GGCGCC GG^CGCC
NspV 1495 TTCGAA TT^CGAA
PaeR7I 1180 CTCGAG C^TCGAG
PmaCI 1288 CACGTG CAC^GTG
PmlI 1288 CACGTG CAC^GTG
PpuMI 2037 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 643 ATTAAT AT^TAAT
RcaI 536 TCATGA T^CATGA
SbfI 795 CCTGCAGG CCTGCA^GG
SfiI 1641 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3025 GGCGCC GGC^GCC
SfuI 1495 TTCGAA TT^CGAA
Sse8387I 795 CCTGCAGG CCTGCA^GG
XbaI 2856 TCTAGA T^CTAGA
XcmI 404 CCAACACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1180 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AspI 1612 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2098 GACCTTGTC
BalI 1253 TGGCCA TGG^CCA
3173 TGGCCA
BglI 1642 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3028 GCCTACGAGGC
BglII 732 AGATCT A^GATCT
1098 AGATCT
BlpI 1582 GCTCAGC GC^T[ACGT]AGC
2920 GCTAAGC
Bpu1102I 1582 GCTCAGC GC^T[ACGT]AGC
2920 GCTAAGC
BsaWI 743 ACCGGA [AT]^CCGG[AT]
2934 ACCGGA
BspLU11I 860 ACATGT A^CATGT
1211 ACATGT
CelII 1582 GCTCAGC GC^T[ACGT]AGC
2920 GCTAAGC
EcoNI 438 CCTTATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2100 CCTTGTCAAGG
EcoT22I 788 ATGCAT ATGCA^T
912 ATGCAT
FauI 701 CCCGC CCCGC(4/6)
1818 GCGGG
HgaI 1753 GACGC GACGC(5/10)
2718 GCGTC
MfeI 680 CAATTG C^AATTG
1389 CAATTG
MluNI 1253 TGGCCA TGG^CCA
3173 TGGCCA
MscI 1253 TGGCCA TGG^CCA
3173 TGGCCA
MunI 680 CAATTG C^AATTG
1389 CAATTG
NcoI 155 CCATGG C^CATGG
2981 CCATGG
NheI 321 GCTAGC G^CTAGC
399 GCTAGC
NsiI 788 ATGCAT ATGCA^T
912 ATGCAT
PflFI 1612 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2098 GACCTTGTC
SexAI 611 ACCTGGT A^CC[AT]GGT
2570 ACCTGGT
SmaI 35 CCCGGG CCC^GGG
1643 CCCGGG
Tth111I 1612 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2098 GACCTTGTC
XmaCI 35 CCCGGG C^CCGGG
1643 CCCGGG
XmaI 35 CCCGGG C^CCGGG
1643 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI