##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwNI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BseAI BsiWI BsmFI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tru9I Tth111I
XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 315 GAATTT [GA]^AATT[CT]
AcyI 581 GACGCC G[GA]^CG[CT]C
Alw44I 390 GTGCAC G^TGCAC
AocI 943 CCTGAGG CC^T[ACGT]AGG
ApaI 636 GGGCCC GGGCC^C
ApaLI 390 GTGCAC G^TGCAC
ApoI 315 GAATTT [GA]^AATT[CT]
BanII 636 GGGCCC G[GA]GC[CT]^C
BbvCI 75 GCTGAGG CCTCAGC(-5/-2)
BglI 762 GCCGTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 686 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 686 GCTCAGC GC^T[ACGT]AGC
BsaHI 581 GACGCC G[GA]^CG[CT]C
BsaI 240 GAGACC GGTCTC(1/5)
BsaWI 550 ACCGGA [AT]^CCGG[AT]
BsiEI 1180 CGGCCG CG[GA][CT]^CG
BsmBI 579 GAGACG CGTCTC(1/5)
Bsp1407I 716 TGTACA T^GTACA
BsrGI 716 TGTACA T^GTACA
BsrI 90 CCAGT ACTGG(1/-1)
BssSI 1038 CTCGTG CACGAG(-5/-1)
Bsu36I 943 CCTGAGG CC^T[ACGT]AGG
CelII 686 GCTCAGC GC^T[ACGT]AGC
DraIII 1203 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1076 CCATGG C^C[GA][CT]GG
EagI 1180 CGGCCG C^GGCCG
EclXI 1180 CGGCCG C^GGCCG
Eco52I 1180 CGGCCG C^GGCCG
Eco81I 943 CCTGAGG CC^T[ACGT]AGG
HgaI 581 GACGC GACGC(5/10)
Hin1I 581 GACGCC G[GA]^CG[CT]C
HindIII 119 AAGCTT A^AGCTT
HinfI 673 GAGTC G^A[ACGT]TC
NaeI 584 GCCGGC GCC^GGC
NcoI 1076 CCATGG C^CATGG
NgoMIV 584 GCCGGC G^CCGGC
PflMI 821 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 673 GAGTC GAGTC(4/5)
SexAI 3 ACCAGGT A^CC[AT]GGT
SfaNI 17 GATGC GCATC(5/9)
SmlI 324 CTTGAG C^T[CT][GA]AG
SspBI 716 TGTACA T^GTACA
TaqI 349 TCGA T^CGA
TthHB8I 349 TCGA T^CGA
Van91I 821 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 817 CCATCCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 59 GGATC GGATC(4/5)
545 GGATC
AspHI 390 GTGCAC G[AT]GC[AT]^C
878 GTGCTC
BcgI 311 CGACGAATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
340 GCAGCCTCTTCG
BsgI 180 CTGCAC GTGCAG(16/14)
423 CTGCAC
BsiHKAI 390 GTGCAC G[AT]GC[AT]^C
878 GTGCTC
BsrBI 627 CCGCTC CCGCTC(-3/-3)
488 GAGCGG
BsrFI 394 ACCGGC [GA]^CCGG[CT]
584 GCCGGC
Cfr10I 394 ACCGGC [GA]^CCGG[CT]
584 GCCGGC
DpnII 60 GATC ^GATC
546 GATC
EaeI 307 TGGCCG [CT]^GGCC[GA]
1180 CGGCCG
MaeII 175 ACGT A^CGT
388 ACGT
MaeIII 1033 GTGAC ^GT[ACGT]AC
1185 GTGAC
MboI 60 GATC ^GATC
546 GATC
NdeII 60 GATC ^GATC
546 GATC
PvuII 540 CAGCTG CAG^CTG
1195 CAGCTG
Sau3AI 60 GATC ^GATC
546 GATC
SmaI 228 CCCGGG CCC^GGG
736 CCCGGG
Tsp45I 1033 GTGAC ^GT[GC]AC
1185 GTGAC
Tsp509I 316 AATT ^AATT
1239 AATT
XmaCI 228 CCCGGG C^CCGGG
736 CCCGGG
XmaI 228 CCCGGG C^CCGGG
736 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfcI StyI
TseI TspRI