##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BanI BbeI
BciVI BclI BfaI BfrI BlnI
BsaBI BsaI BsaWI BseAI BsiWI
BspDI BspEI BspLU11I BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 1211 GACGCC G[GA]^CG[CT]C
Aor51HI 518 AGCGCT AGC^GCT
AspHI 595 GAGCTC G[AT]GC[AT]^C
BalI 1175 TGGCCA TGG^CCA
BamHI 1922 GGATCC G^GATCC
BbrPI 1192 CACGTG CAC^GTG
BbsI 864 GAAGAC GAAGAC(2/6)
BglII 552 AGATCT A^GATCT
BpuAI 864 GAAGAC GAAGAC(2/6)
BsaHI 1211 GACGCC G[GA]^CG[CT]C
BsiEI 372 CGGCCG CG[GA][CT]^CG
BsiHKAI 595 GAGCTC G[AT]GC[AT]^C
BsmI 507 GAATGC GAATGC(1/-1)
Bsp1407I 1863 TGTACA T^GTACA
BspHI 1539 TCATGA T^CATGA
BsrDI 1268 GCAATG GCAATG(2/0)
BsrGI 1863 TGTACA T^GTACA
BstAPI 115 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 372 CGGCCG C^GGCCG
EclXI 372 CGGCCG C^GGCCG
Eco47III 518 AGCGCT AGC^GCT
Eco52I 372 CGGCCG C^GGCCG
Hin1I 1211 GACGCC G[GA]^CG[CT]C
MluNI 1175 TGGCCA TGG^CCA
MscI 1175 TGGCCA TGG^CCA
MslI 1730 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 2069 ACATGC [GA]CATG^[CT]
PaeR7I 1373 CTCGAG C^TCGAG
PmaCI 1192 CACGTG CAC^GTG
PmlI 1192 CACGTG CAC^GTG
RcaI 1539 TCATGA T^CATGA
SacI 595 GAGCTC GAGCT^C
SfiI 1167 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1863 TGTACA T^GTACA
SspI 1930 AATATT AAT^ATT
XhoI 1373 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 235 CGCG CG^CG
1230 CGCG
ApaI 613 GGGCCC GGGCC^C
1090 GGGCCC
AvaI 439 CCCGAG C^[CT]CG[GA]G
1373 CTCGAG
BlpI 99 GCTGAGC GC^T[ACGT]AGC
1464 GCTCAGC
Bpu1102I 99 GCTGAGC GC^T[ACGT]AGC
1464 GCTCAGC
BsmBI 399 CGTCTC CGTCTC(1/5)
1491 GAGACG
BsoBI 439 CCCGAG C^[CT]CG[GA]G
1373 CTCGAG
BssSI 592 CACGAG CACGAG(-5/-1)
1936 CTCGTG
BstUI 235 CGCG CG^CG
1230 CGCG
CelII 99 GCTGAGC GC^T[ACGT]AGC
1464 GCTCAGC
HaeII 179 AGCGCC [GA]GCGC^[CT]
518 AGCGCT
MvnI 235 CGCG CG^CG
1230 CGCG
NaeI 370 GCCGGC GCC^GGC
374 GCCGGC
NgoMIV 370 GCCGGC G^CCGGC
374 GCCGGC
PleI 1332 GAGTC GAGTC(4/5)
1376 GAGTC
PpuMI 389 GGGTCCT [GA]G^G[AT]CC[CT]
644 AGGACCT
TaqI 88 TCGA T^CGA
1374 TCGA
TthHB8I 88 TCGA T^CGA
1374 TCGA
XcmI 212 CCAGATCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1163 CCAAGGCCGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvCI BbvI BcgI
BcnI BglI BmyI BpmI BsaAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII