##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI AspEI AspI
AviII BbvCI BcgI BclI BfrI
BsaBI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DraIII Eam1105I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NsiI NspI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 3372 GGTACC G^GTACC
Alw44I 1477 GTGCAC G^TGCAC
AocI 665 CCTCAGG CC^T[ACGT]AGG
ApaLI 1477 GTGCAC G^TGCAC
Asp718I 3372 GGTACC G^GTACC
AvrII 682 CCTAGG C^CTAGG
BalI 392 TGGCCA TGG^CCA
BbsI 1113 GAAGAC GAAGAC(2/6)
BciVI 3353 GGATAC GTATCC(6/5)
BlnI 682 CCTAGG C^CTAGG
BpuAI 1113 GAAGAC GAAGAC(2/6)
BsaWI 1545 TCCGGT [AT]^CCGG[AT]
BspHI 2486 TCATGA T^CATGA
BssHII 1372 GCGCGC G^CGCGC
BssSI 2315 CACGAG CACGAG(-5/-1)
Bsu36I 665 CCTCAGG CC^T[ACGT]AGG
Eco81I 665 CCTCAGG CC^T[ACGT]AGG
KpnI 3372 GGTACC GGTAC^C
MluNI 392 TGGCCA TGG^CCA
MscI 392 TGGCCA TGG^CCA
NruI 242 TCGCGA TCG^CGA
PaeR7I 1063 CTCGAG C^TCGAG
PflMI 1754 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2486 TCATGA T^CATGA
SacI 1714 GAGCTC GAGCT^C
SapI 892 GAAGAGC GCTCTTC(1/4)
TfiI 3399 GAATC G^A[AT]TC
Tsp509I 3446 AATT ^AATT
Van91I 1754 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2138 CCACTCGGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1063 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Aor51HI 1079 AGCGCT AGC^GCT
1139 AGCGCT
Asp700I 234 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2727 GAATGGCTTC
BamHI 2879 GGATCC G^GATCC
3314 GGATCC
BbrPI 1344 CACGTG CAC^GTG
1682 CACGTG
BglII 3041 AGATCT A^GATCT
3279 AGATCT
BlpI 1206 GCTGAGC GC^T[ACGT]AGC
1716 GCTCAGC
Bpu1102I 1206 GCTGAGC GC^T[ACGT]AGC
1716 GCTCAGC
BsaAI 1344 CACGTG [CT]AC^GT[GA]
1682 CACGTG
BstEII 544 GGTGACC G^GT[ACGT]ACC
2091 GGTGACC
BstPI 544 GGTGACC G^GT[ACGT]ACC
2091 GGTGACC
BstXI 902 CCAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2721 CCAGGAGAATGG
CelII 1206 GCTGAGC GC^T[ACGT]AGC
1716 GCTCAGC
DrdI 2050 GACGCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2496 GACTCGGAAGTC
Eco47III 1079 AGCGCT AGC^GCT
1139 AGCGCT
EcoO65I 544 GGTGACC G^GT[ACGT]ACC
2091 GGTGACC
KspI 375 CCGCGG CCGC^GG
2033 CCGCGG
MslI 2888 CACAGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3058 CATTCGTGTG
NcoI 452 CCATGG C^CATGG
752 CCATGG
PmaCI 1344 CACGTG CAC^GTG
1682 CACGTG
PmlI 1344 CACGTG CAC^GTG
1682 CACGTG
SacII 375 CCGCGG CCGC^GG
2033 CCGCGG
StuI 1565 AGGCCT AGG^CCT
1768 AGGCCT
XmnI 234 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2727 GAATGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SgrAI SmaI
SmlI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I XhoII XmaCI
XmaI