##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KspI MaeII MamI
MfeI MluI MluNI MscI MslI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 848 GGTACC G^GTACC
AccIII 335 TCCGGA T^CCGGA
AciI 870 CCGC CCGC(-3/-1)
AcsI 125 AAATTT [GA]^AATT[CT]
AfaI 849 GTAC GT^AC
AlwNI 818 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 125 AAATTT [GA]^AATT[CT]
Asp718I 848 GGTACC G^GTACC
AvaII 892 GGACC G^G[AT]CC
BbvCI 215 GCTGAGG CCTCAGC(-5/-2)
BcnI 670 CCCGG CC^[GC]GG
BsaWI 335 TCCGGA [AT]^CCGG[AT]
BseAI 335 TCCGGA T^CCGGA
BsmFI 943 GTCCC GGGAC(10/14)
BspEI 335 TCCGGA T^CCGGA
BspHI 190 TCATGA T^CATGA
BsrBI 870 CCGCTC CCGCTC(-3/-3)
Cac8I 809 GCCAGC GC[ACGT]^[ACGT]GC
DsaI 927 CCATGG C^C[GA][CT]GG
EaeI 33 TGGCCG [CT]^GGCC[GA]
EcoNI 577 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 869 CCCGC CCCGC(4/6)
HincII 56 GTTAAC GT[CT]^[GA]AC
HindII 56 GTTAAC GT[CT]^[GA]AC
HindIII 75 AAGCTT A^AGCTT
HpaI 56 GTTAAC GTT^AAC
KpnI 848 GGTACC GGTAC^C
MroI 335 TCCGGA T^CCGGA
MspA1I 508 CAGCTG C[AC]G^C[GT]G
NciI 670 CCCGG CC^[GC]GG
NcoI 927 CCATGG C^CATGG
PflMI 469 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 642 CTGCAG CTGCA^G
PvuII 508 CAGCTG CAG^CTG
RcaI 190 TCATGA T^CATGA
RsaI 849 GTAC GT^AC
SapI 704 GAAGAGC GCTCTTC(1/4)
SexAI 816 ACCAGGT A^CC[AT]GGT
SfaNI 212 GATGC GCATC(5/9)
SmlI 297 CTCAAG C^T[CT][GA]AG
StuI 550 AGGCCT AGG^CCT
TaqI 702 TCGA T^CGA
Tsp45I 537 GTCAC ^GT[GC]AC
TthHB8I 702 TCGA T^CGA
Van91I 469 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 676 GGATC GGATC(4/5)
697 GGATC
BanI 784 GGTGCC G^G[CT][GA]CC
848 GGTACC
BbsI 455 GAAGAC GAAGAC(2/6)
665 GAAGAC
BfaI 245 CTAG C^TAG
983 CTAG
BpuAI 455 GAAGAC GAAGAC(2/6)
665 GAAGAC
BsaI 524 GAGACC GGTCTC(1/5)
908 GAGACC
BstF5I 106 CATCC GGATG(2/0)
211 GGATG
BstYI 676 GGATCT [GA]^GATC[CT]
697 GGATCT
Cfr13I 880 GGCCC G^G[ACGT]CC
892 GGACC
EcoT14I 529 CCTTGG C^C[AT][AT]GG
927 CCATGG
FokI 106 CATCC GGATG(9/13)
211 GGATG
HapII 336 CCGG C^CGG
671 CCGG
HpaII 336 CCGG C^CGG
671 CCGG
HphI 8 TCACC GGTGA(8/7)
185 TCACC
MaeI 245 CTAG C^TAG
983 CTAG
MaeIII 537 GTCAC ^GT[ACGT]AC
995 GTAAC
MflI 676 GGATCT [GA]^GATC[CT]
697 GGATCT
MspI 336 CCGG C^CGG
671 CCGG
Sau96I 880 GGCCC G^G[ACGT]CC
892 GGACC
SfcI 69 CTGTAG C^T[GA][CT]AG
642 CTGCAG
StyI 529 CCTTGG C^C[AT][AT]GG
927 CCATGG
Tsp509I 126 AATT ^AATT
486 AATT
XhoII 676 GGATCT [GA]^GATC[CT]
697 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI BbvI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsrI BssKI
BstNI DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HinfI ItaI Ksp632I
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI ScrFI TfiI Tru9I TseI
TspRI