##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Aor51HI ApaI
AscI AspEI AspI AvaI AviII
BamHI BbeI BbrPI BcgI BciVI
BclI BglI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstUI
BstXI BstZ17I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KspI
MamI MluI MroI MvnI MwoI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I StuI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3298 GGTACC G^GTACC
AflII 2829 CTTAAG C^TTAAG
AflIII 554 ACATGT A^C[GA][CT]GT
Alw44I 200 GTGCAC G^TGCAC
AlwI 2593 GATCC GGATC(4/5)
AlwNI 1361 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 200 GTGCAC G^TGCAC
AseI 3318 ATTAAT AT^TAAT
AsnI 3318 ATTAAT AT^TAAT
Asp718I 3298 GGTACC G^GTACC
BalI 3123 TGGCCA TGG^CCA
BanI 3298 GGTACC G^G[CT][GA]CC
BanII 1915 GAGCTC G[GA]GC[CT]^C
BbvCI 1953 CCTCAGC CCTCAGC(-5/-2)
BcnI 2333 CCGGG CC^[GC]GG
BfrI 2829 CTTAAG C^TTAAG
BglII 333 AGATCT A^GATCT
BseRI 577 GAGGAG GAGGAG(10/8)
BsmI 1819 GAATGC GAATGC(1/-1)
Bsp1407I 1299 TGTACA T^GTACA
BspHI 47 TCATGA T^CATGA
BspLU11I 554 ACATGT A^CATGT
BsrDI 1211 GCAATG GCAATG(2/0)
BsrGI 1299 TGTACA T^GTACA
BstBI 533 TTCGAA TT^CGAA
EaeI 3123 TGGCCA [CT]^GGCC[GA]
EcoRV 793 GATATC GAT^ATC
EcoT22I 892 ATGCAT ATGCA^T
HindIII 1092 AAGCTT A^AGCTT
KpnI 3298 GGTACC GGTAC^C
MaeII 2020 ACGT A^CGT
MfeI 1518 CAATTG C^AATTG
MluNI 3123 TGGCCA TGG^CCA
MscI 3123 TGGCCA TGG^CCA
MspA1I 509 CAGCTG C[AC]G^C[GT]G
MunI 1518 CAATTG C^AATTG
NciI 2333 CCGGG CC^[GC]GG
NlaIV 3298 GGTACC GG[ACGT]^[ACGT]CC
NsiI 892 ATGCAT ATGCA^T
NspV 533 TTCGAA TT^CGAA
PshAI 3153 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3318 ATTAAT AT^TAAT
PvuII 509 CAGCTG CAG^CTG
RcaI 47 TCATGA T^CATGA
SacI 1915 GAGCTC GAGCT^C
SfuI 533 TTCGAA TT^CGAA
SspBI 1299 TGTACA T^GTACA
SwaI 3506 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 40 CCGC CCGC(-3/-1)
3033 CCGC
AocI 776 CCTTAGG CC^T[ACGT]AGG
3098 CCTCAGG
AvrII 1473 CCTAGG C^CTAGG
3144 CCTAGG
BbsI 431 GAAGAC GAAGAC(2/6)
2196 GTCTTC
BlnI 1473 CCTAGG C^CTAGG
3144 CCTAGG
BpmI 23 CTCCAG CTGGAG(16/14)
798 CTCCAG
BpuAI 431 GAAGAC GAAGAC(2/6)
2196 GTCTTC
BsmAI 1239 GAGAC GTCTC(1/5)
2044 GAGAC
BstEII 499 GGTCACC G^GT[ACGT]ACC
2790 GGTGACC
BstPI 499 GGTCACC G^GT[ACGT]ACC
2790 GGTGACC
BstYI 333 AGATCT [GA]^GATC[CT]
2592 AGATCC
Bsu36I 776 CCTTAGG CC^T[ACGT]AGG
3098 CCTCAGG
DsaI 266 CCATGG C^C[GA][CT]GG
3020 CCATGG
Eco81I 776 CCTTAGG CC^T[ACGT]AGG
3098 CCTCAGG
EcoO65I 499 GGTCACC G^GT[ACGT]ACC
2790 GGTGACC
EcoRI 877 GAATTC G^AATTC
2400 GAATTC
HaeIII 3024 GGCC GG^CC
3124 GGCC
HapII 61 CCGG C^CGG
2333 CCGG
HpaII 61 CCGG C^CGG
2333 CCGG
MflI 333 AGATCT [GA]^GATC[CT]
2592 AGATCC
MspI 61 CCGG C^CGG
2333 CCGG
NcoI 266 CCATGG C^CATGG
3020 CCATGG
SmlI 2829 CTTAAG C^T[CT][GA]AG
3255 CTTGAG
SpeI 352 ACTAGT A^CTAGT
1838 ACTAGT
SspI 1873 AATATT AAT^ATT
2028 AATATT
XhoII 333 AGATCT [GA]^GATC[CT]
2592 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AspHI AvaII BbvI BfaI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I Fnu4HI FokI HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI