##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaI AviII BamHI
BbrPI BcnI BfrI BglII BlpI
Bpu1102I BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI TaqI Tth111I TthHB8I Van91I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccI 1359 GTCTAC GT^[AC][GT]AC
AciI 1513 GCGG CCGC(-3/-1)
AcsI 1534 AAATTT [GA]^AATT[CT]
AlwNI 1494 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 466 CCTGAGG CC^T[ACGT]AGG
ApaI 249 GGGCCC GGGCC^C
ApoI 1534 AAATTT [GA]^AATT[CT]
AvrII 883 CCTAGG C^CTAGG
BalI 493 TGGCCA TGG^CCA
BanI 555 GGCGCC G^G[CT][GA]CC
BbeI 555 GGCGCC GGCGC^C
BbsI 1788 GTCTTC GAAGAC(2/6)
BbvCI 583 GCTGAGG CCTCAGC(-5/-2)
BcgI 589 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 461 GGATAC GTATCC(6/5)
BclI 898 TGATCA T^GATCA
BlnI 883 CCTAGG C^CTAGG
BpuAI 1788 GTCTTC GAAGAC(2/6)
BsaAI 153 TACGTG [CT]AC^GT[GA]
BsaBI 208 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 1647 TGTACA T^GTACA
BspMI 1686 ACCTGC ACCTGC(4/8)
BsrGI 1647 TGTACA T^GTACA
Bsu36I 466 CCTGAGG CC^T[ACGT]AGG
CfoI 556 GCGC GCG^C
EaeI 493 TGGCCA [CT]^GGCC[GA]
EarI 1637 CTCTTC CTCTTC(1/4)
Eco81I 466 CCTGAGG CC^T[ACGT]AGG
FauI 1513 GCGGG CCCGC(4/6)
FbaI 898 TGATCA T^GATCA
HaeII 555 GGCGCC [GA]GCGC^[CT]
HapII 1233 CCGG C^CGG
HgaI 674 GACGC GACGC(5/10)
HhaI 556 GCGC GCG^C
HinP1I 556 GCGC G^CGC
HincII 1464 GTTAAC GT[CT]^[GA]AC
HindII 1464 GTTAAC GT[CT]^[GA]AC
HindIII 772 AAGCTT A^AGCTT
HpaI 1464 GTTAAC GTT^AAC
HpaII 1233 CCGG C^CGG
KasI 555 GGCGCC G^GCGCC
Ksp632I 1637 CTCTTC CTCTTC(1/4)
MaeII 154 ACGT A^CGT
MamI 208 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 493 TGGCCA TGG^CCA
MscI 493 TGGCCA TGG^CCA
MspA1I 403 CAGCTG C[AC]G^C[GT]G
MspI 1233 CCGG C^CGG
NarI 555 GGCGCC GG^CGCC
NcoI 6 CCATGG C^CATGG
NspI 1242 GCATGC [GA]CATG^[CT]
PvuII 403 CAGCTG CAG^CTG
SfaNI 343 GCATC GCATC(5/9)
SfcI 560 CTGTAG C^T[GA][CT]AG
SfiI 1216 GGCCTCTTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 555 GGCGCC GGC^GCC
SphI 1242 GCATGC GCATG^C
SspBI 1647 TGTACA T^GTACA
SspI 827 AATATT AAT^ATT
StuI 111 AGGCCT AGG^CCT
XbaI 300 TCTAGA T^CTAGA
XcmI 1080 CCAAGGTTTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 555 GGCGCC G[GA]^CG[CT]C
674 GACGCC
AseI 1310 ATTAAT AT^TAAT
1774 ATTAAT
AsnI 1310 ATTAAT AT^TAAT
1774 ATTAAT
BbvI 188 GCAGC GCAGC(8/12)
1196 GCAGC
BglI 104 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1217 GCCTCTTAGGC
BsaHI 555 GGCGCC G[GA]^CG[CT]C
674 GACGCC
BsmBI 513 CGTCTC CGTCTC(1/5)
672 GAGACG
BstYI 71 AGATCC [GA]^GATC[CT]
181 AGATCC
DsaI 6 CCATGG C^C[GA][CT]GG
245 CCACGG
EcoNI 1482 CCTTGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1596 CCTTACTGAGG
Fnu4HI 188 GCAGC GC^[ACGT]GC
1196 GCAGC
Hin1I 555 GGCGCC G[GA]^CG[CT]C
674 GACGCC
HphI 503 GGTGA GGTGA(8/7)
1558 TCACC
ItaI 188 GCAGC GC^[ACGT]GC
1196 GCAGC
MaeIII 270 GTGAC ^GT[ACGT]AC
1521 GTCAC
MflI 71 AGATCC [GA]^GATC[CT]
181 AGATCC
MslI 214 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1559 CACCAAAATG
PshBI 1310 ATTAAT AT^TAAT
1774 ATTAAT
SmlI 1151 CTTGAG C^T[CT][GA]AG
1438 CTTGAG
TseI 188 GCAGC G^C[AT]GC
1196 GCAGC
Tsp45I 270 GTGAC ^GT[GC]AC
1521 GTCAC
XhoII 71 AGATCC [GA]^GATC[CT]
181 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AfaI AluI AlwI AspHI AvaII
BanII BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraI DraII EcoO109I EcoRII
EcoT14I FokI HaeIII HinfI MaeI
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
StyI TfiI Tru9I Tsp509I TspRI