##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AhdI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BlnI BsaAI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AgeI 951 ACCGGT A^CCGGT
Alw44I 101 GTGCAC G^TGCAC
AocI 1138 CCTCAGG CC^T[ACGT]AGG
ApaLI 101 GTGCAC G^TGCAC
BalI 562 TGGCCA TGG^CCA
BamHI 652 GGATCC G^GATCC
BanI 954 GGTGCC G^G[CT][GA]CC
BbsI 1006 GAAGAC GAAGAC(2/6)
BbvCI 1326 CCTCAGC CCTCAGC(-5/-2)
BglII 324 AGATCT A^GATCT
BlpI 710 GCTCAGC GC^T[ACGT]AGC
BpmI 263 CTCCAG CTGGAG(16/14)
Bpu1102I 710 GCTCAGC GC^T[ACGT]AGC
BpuAI 1006 GAAGAC GAAGAC(2/6)
BsaBI 1217 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 951 ACCGGT [AT]^CCGG[AT]
BsmAI 53 GAGAC GTCTC(1/5)
BsmBI 53 GAGACG CGTCTC(1/5)
BsmFI 73 GTCCC GGGAC(10/14)
BsmI 969 GAATGC GAATGC(1/-1)
BspHI 1228 TCATGA T^CATGA
BspMI 163 ACCTGC ACCTGC(4/8)
BsrDI 1394 CATTGC GCAATG(2/0)
BsrFI 951 ACCGGT [GA]^CCGG[CT]
Bsu36I 1138 CCTCAGG CC^T[ACGT]AGG
CelII 710 GCTCAGC GC^T[ACGT]AGC
CfoI 1146 GCGC GCG^C
Cfr10I 951 ACCGGT [GA]^CCGG[CT]
DraII 148 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 562 TGGCCA [CT]^GGCC[GA]
Eco81I 1138 CCTCAGG CC^T[ACGT]AGG
EcoO109I 148 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 302 GATATC GAT^ATC
HapII 952 CCGG C^CGG
HgaI 55 GACGC GACGC(5/10)
HhaI 1146 GCGC GCG^C
HinP1I 1146 GCGC G^CGC
HpaII 952 CCGG C^CGG
HphI 949 TCACC GGTGA(8/7)
MaeII 444 ACGT A^CGT
MamI 1217 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 562 TGGCCA TGG^CCA
MscI 562 TGGCCA TGG^CCA
MseI 747 TTAA T^TAA
MslI 743 CACCTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 952 CCGG C^CGG
PinAI 951 ACCGGT A^CCGGT
PpuMI 148 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1228 TCATGA T^CATGA
SacI 434 GAGCTC GAGCT^C
SgrAI 950 CACCGGTG C[GA]^CCGG[CT]G
Tru9I 747 TTAA T^TAA
XcmI 1260 CCACTGCTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 71 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
203 CAGCACCTG
AspHI 101 GTGCAC G[AT]GC[AT]^C
434 GAGCTC
BanII 434 GAGCTC G[GA]GC[CT]^C
1066 GAGCCC
BclI 78 TGATCA T^GATCA
423 TGATCA
BsiHKAI 101 GTGCAC G[AT]GC[AT]^C
434 GAGCTC
BsrBI 355 CCGCTC CCGCTC(-3/-3)
462 CCGCTC
EarI 1340 CTCTTC CTCTTC(1/4)
533 GAAGAG
EcoT14I 900 CCTTGG C^C[AT][AT]GG
907 CCTTGG
FauI 354 CCCGC CCCGC(4/6)
1134 CCCGC
FbaI 78 TGATCA T^GATCA
423 TGATCA
Ksp632I 1340 CTCTTC CTCTTC(1/4)
533 GAAGAG
NspI 1010 ACATGC [GA]CATG^[CT]
1437 ACATGC
PstI 480 CTGCAG CTGCA^G
674 CTGCAG
SmlI 858 CTTGAG C^T[CT][GA]AG
1417 CTCAAG
StyI 900 CCTTGG C^C[AT][AT]GG
907 CCTTGG
Tsp509I 935 AATT ^AATT
1470 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BfaI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeIII
MboI MboII MflI MnlI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI TseI Tsp45I TspRI XbaI
XhoII