##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BciVI BglI BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BspEI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI CelII Cfr10I CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Alw44I 4307 GTGCAC G^TGCAC
ApaI 1942 GGGCCC GGGCC^C
ApaLI 4307 GTGCAC G^TGCAC
BalI 2171 TGGCCA TGG^CCA
BanI 4871 GGCACC G^G[CT][GA]CC
BbvCI 1916 CCTCAGC CCTCAGC(-5/-2)
BclI 5935 TGATCA T^GATCA
BcnI 1032 CCCGG CC^[GC]GG
BglII 1657 AGATCT A^GATCT
BsaAI 5947 CACGTA [CT]AC^GT[GA]
BspDI 1435 ATCGAT AT^CGAT
BspMI 5444 GCAGGT ACCTGC(4/8)
BsrBI 128 GAGCGG CCGCTC(-3/-3)
Bst1107I 466 GTATAC GTA^TAC
BstZ17I 466 GTATAC GTA^TAC
ClaI 1435 ATCGAT AT^CGAT
DsaI 1341 CCATGG C^C[GA][CT]GG
EaeI 2171 TGGCCA [CT]^GGCC[GA]
EcoRV 1124 GATATC GAT^ATC
EcoT22I 1508 ATGCAT ATGCA^T
FbaI 5935 TGATCA T^GATCA
HapII 1033 CCGG C^CGG
HpaI 4966 GTTAAC GTT^AAC
HpaII 1033 CCGG C^CGG
MluNI 2171 TGGCCA TGG^CCA
MscI 2171 TGGCCA TGG^CCA
MspI 1033 CCGG C^CGG
NciI 1032 CCCGG CC^[GC]GG
NcoI 1341 CCATGG C^CATGG
NsiI 1508 ATGCAT ATGCA^T
PaeR7I 394 CTCGAG C^TCGAG
StuI 799 AGGCCT AGG^CCT
XbaI 1223 TCTAGA T^CTAGA
XhoI 394 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 3 CGCG CG^CG
47 CGCG
AcyI 21 GGCGTC G[GA]^CG[CT]C
3702 GACGCC
AflII 550 CTTAAG C^TTAAG
2194 CTTAAG
AocI 1929 CCTTAGG CC^T[ACGT]AGG
2800 CCTTAGG
AspI 1234 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3484 GACTAAGTC
AvaI 247 CCCGAG C^[CT]CG[GA]G
394 CTCGAG
BcgI 313 CGAAGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1437 CGATTGAAATGC
BfrI 550 CTTAAG C^TTAAG
2194 CTTAAG
BsaHI 21 GGCGTC G[GA]^CG[CT]C
3702 GACGCC
BsoBI 247 CCCGAG C^[CT]CG[GA]G
394 CTCGAG
Bsp1407I 2926 TGTACA T^GTACA
3594 TGTACA
BsrGI 2926 TGTACA T^GTACA
3594 TGTACA
BstUI 3 CGCG CG^CG
47 CGCG
Bsu36I 1929 CCTTAGG CC^T[ACGT]AGG
2800 CCTTAGG
Eco81I 1929 CCTTAGG CC^T[ACGT]AGG
2800 CCTTAGG
EcoRI 618 GAATTC G^AATTC
3313 GAATTC
EcoT14I 935 CCAAGG C^C[AT][AT]GG
1341 CCATGG
HaeII 144 GGCGCT [GA]GCGC^[CT]
1063 GGCGCT
HgaI 3702 GACGC GACGC(5/10)
22 GCGTC
Hin1I 21 GGCGTC G[GA]^CG[CT]C
3702 GACGCC
HindIII 547 AAGCTT A^AGCTT
638 AAGCTT
MaeII 1192 ACGT A^CGT
5948 ACGT
MvnI 3 CGCG CG^CG
47 CGCG
PflFI 1234 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3484 GACTAAGTC
PpuMI 1515 GGGACCC [GA]G^G[AT]CC[CT]
3447 AGGTCCC
PstI 148 CTGCAG CTGCA^G
3681 CTGCAG
SapI 314 GAAGAGC GCTCTTC(1/4)
5492 GCTCTTC
ScaI 1411 AGTACT AGT^ACT
5906 AGTACT
SpeI 4704 ACTAGT A^CTAGT
5297 ACTAGT
SphI 423 GCATGC GCATG^C
3073 GCATGC
SspBI 2926 TGTACA T^GTACA
3594 TGTACA
StyI 935 CCAAGG C^C[AT][AT]GG
1341 CCATGG
SwaI 732 ATTTAAAT ATTT^AAAT
5282 ATTTAAAT
Tth111I 1234 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3484 GACTAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspLU11I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NheI NlaIII
NlaIV NspI PflMI PleI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI