##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
Aor51HI AscI AseI AsnI AviII
AvrII BamHI BclI BglI BlnI
BsaWI BseAI BsiEI BsiWI BspEI
BsrBI BsrFI BssHII BstEII BstPI
BstUI Cfr10I CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MfeI MluI MroI
MunI MvnI NaeI NdeI NgoMIV
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
ScaI SfiI SgrAI SmaI SnaBI
SphI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AccI 5871 GTATAC GT^[AC][GT]AC
ApaI 2852 GGGCCC GGGCC^C
BbrPI 1780 CACGTG CAC^GTG
BcnI 314 CCCGG CC^[GC]GG
BsaAI 1780 CACGTG [CT]AC^GT[GA]
BsaBI 5145 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1882 CTGCAC GTGCAG(16/14)
BsmI 3453 GAATGC GAATGC(1/-1)
BspDI 1940 ATCGAT AT^CGAT
Bst1107I 5871 GTATAC GTA^TAC
BstBI 2684 TTCGAA TT^CGAA
BstZ17I 5871 GTATAC GTA^TAC
ClaI 1940 ATCGAT AT^CGAT
DraI 3788 TTTAAA TTT^AAA
DraIII 3395 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 2124 GAATTC G^AATTC
HgaI 2183 GCGTC GACGC(5/10)
MamI 5145 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 314 CCCGG CC^[GC]GG
NheI 1020 GCTAGC G^CTAGC
NspV 2684 TTCGAA TT^CGAA
PmaCI 1780 CACGTG CAC^GTG
PmlI 1780 CACGTG CAC^GTG
SexAI 1046 ACCTGGT A^CC[AT]GGT
SfuI 2684 TTCGAA TT^CGAA
SpeI 4283 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 409 GGCGCC G[GA]^CG[CT]C
3523 GGCGCC
AflII 2863 CTTAAG C^TTAAG
3323 CTTAAG
AhdI 1906 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3281 GACAAAAAGTC
Alw44I 1348 GTGCAC G^TGCAC
5296 GTGCAC
AocI 4027 CCTCAGG CC^T[ACGT]AGG
4947 CCTAAGG
ApaLI 1348 GTGCAC G^TGCAC
5296 GTGCAC
Asp700I 4432 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5213 GAAGGCTTTC
AspEI 1906 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3281 GACAAAAAGTC
AvaI 2486 CCCGAG C^[CT]CG[GA]G
3934 CCCGAG
BbeI 409 GGCGCC GGCGC^C
3523 GGCGCC
BbsI 3144 GAAGAC GAAGAC(2/6)
3580 GAAGAC
BcgI 3914 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1239 GCAGGACGTTCG
BfrI 2863 CTTAAG C^TTAAG
3323 CTTAAG
BglII 1593 AGATCT A^GATCT
2327 AGATCT
BpuAI 3144 GAAGAC GAAGAC(2/6)
3580 GAAGAC
BsaHI 409 GGCGCC G[GA]^CG[CT]C
3523 GGCGCC
BsmBI 2535 CGTCTC CGTCTC(1/5)
3981 GAGACG
BsoBI 2486 CCCGAG C^[CT]CG[GA]G
3934 CCCGAG
BspHI 747 TCATGA T^CATGA
758 TCATGA
Bsu36I 4027 CCTCAGG CC^T[ACGT]AGG
4947 CCTAAGG
DrdI 3039 GACGGTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3321 GACTTAAGGGTC
Eam1105I 1906 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3281 GACAAAAAGTC
Eco81I 4027 CCTCAGG CC^T[ACGT]AGG
4947 CCTAAGG
EcoRV 194 GATATC GAT^ATC
943 GATATC
HapII 315 CCGG C^CGG
2053 CCGG
Hin1I 409 GGCGCC G[GA]^CG[CT]C
3523 GGCGCC
HpaII 315 CCGG C^CGG
2053 CCGG
KasI 409 GGCGCC G^GCGCC
3523 GGCGCC
MspI 315 CCGG C^CGG
2053 CCGG
NarI 409 GGCGCC GG^CGCC
3523 GGCGCC
RcaI 747 TCATGA T^CATGA
758 TCATGA
SbfI 666 CCTGCAGG CCTGCA^GG
804 CCTGCAGG
SfoI 409 GGCGCC GGC^GCC
3523 GGCGCC
Sse8387I 666 CCTGCAGG CCTGCA^GG
804 CCTGCAGG
StuI 2701 AGGCCT AGG^CCT
2902 AGGCCT
XbaI 4774 TCTAGA T^CTAGA
5893 TCTAGA
XmnI 4432 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5213 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp718I
AspHI AspI AvaII BalI BanI
BanII BbvCI BbvI BciVI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HinfI HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII