##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AatII AgeI AhdI Aor51HI AscI
AspEI AviII BsiWI Bsp1407I BspDI
BspHI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraIII Eam1105I
Eco47III FseI FspI HpaI MluI
NdeI NotI NruI NspV PacI
PinAI PmeI PshAI PvuI RcaI
RsrII SacI SalI SfuI SgrAI
SnaBI SpeI SspBI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2151 GGTACC G^GTACC
AccI 6478 GTCTAC GT^[AC][GT]AC
AclI 2409 AACGTT AA^CGTT
AflII 4785 CTTAAG C^TTAAG
Alw44I 5188 GTGCAC G^TGCAC
ApaLI 5188 GTGCAC G^TGCAC
Asp718I 2151 GGTACC G^GTACC
AspI 6105 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4642 CCTAGG C^CTAGG
BcgI 3398 CGATCCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4785 CTTAAG C^TTAAG
BlnI 4642 CCTAGG C^CTAGG
BsiEI 134 CGGCCG CG[GA][CT]^CG
BstEII 5891 GGTCACC G^GT[ACGT]ACC
BstPI 5891 GGTCACC G^GT[ACGT]ACC
DrdI 5111 GACGGAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 134 CGGCCG C^GGCCG
EclXI 134 CGGCCG C^GGCCG
Eco52I 134 CGGCCG C^GGCCG
EcoO65I 5891 GGTCACC G^GT[ACGT]ACC
EcoT22I 5650 ATGCAT ATGCA^T
HgaI 4252 GCGTC GACGC(5/10)
HincII 882 GTCAAC GT[CT]^[GA]AC
HindII 882 GTCAAC GT[CT]^[GA]AC
HindIII 1286 AAGCTT A^AGCTT
KpnI 2151 GGTACC GGTAC^C
KspI 88 CCGCGG CCGC^GG
NheI 1354 GCTAGC G^CTAGC
NsiI 5650 ATGCAT ATGCA^T
PaeR7I 296 CTCGAG C^TCGAG
PflFI 6105 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2409 AACGTT AA^CGTT
SacII 88 CCGCGG CCGC^GG
SbfI 5275 CCTGCAGG CCTGCA^GG
SexAI 1977 ACCTGGT A^CC[AT]GGT
SfiI 135 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 5582 CCCGGG CCC^GGG
SphI 5652 GCATGC GCATG^C
Sse8387I 5275 CCTGCAGG CCTGCA^GG
StuI 5027 AGGCCT AGG^CCT
Tth111I 6105 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 739 TCTAGA T^CTAGA
XcmI 2825 CCACTGGGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 296 CTCGAG C^TCGAG
XmaCI 5582 CCCGGG C^CCGGG
XmaI 5582 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 855 TCCGGA T^CCGGA
3289 TCCGGA
AcyI 5325 GGCGCC G[GA]^CG[CT]C
5597 GGCGCC
ApaI 143 GGGCCC GGGCC^C
4801 GGGCCC
BamHI 2386 GGATCC G^GATCC
4688 GGATCC
BbeI 5325 GGCGCC GGCGC^C
5597 GGCGCC
BbvCI 4083 CCTCAGC CCTCAGC(-5/-2)
6014 GCTGAGG
BclI 1961 TGATCA T^GATCA
6126 TGATCA
BlpI 2326 GCTCAGC GC^T[ACGT]AGC
2576 GCTCAGC
Bpu1102I 2326 GCTCAGC GC^T[ACGT]AGC
2576 GCTCAGC
BsaHI 5325 GGCGCC G[GA]^CG[CT]C
5597 GGCGCC
BseAI 855 TCCGGA T^CCGGA
3289 TCCGGA
BsmI 774 GAATGC GAATGC(1/-1)
5648 GAATGC
BspEI 855 TCCGGA T^CCGGA
3289 TCCGGA
BspLU11I 496 ACATGT A^CATGT
5231 ACATGT
BsrFI 136 GCCGGC [GA]^CCGG[CT]
1666 GCCGGC
BstAPI 3505 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5269 GCATGGCCTGC
CelII 2326 GCTCAGC GC^T[ACGT]AGC
2576 GCTCAGC
Cfr10I 136 GCCGGC [GA]^CCGG[CT]
1666 GCCGGC
DraI 5417 TTTAAA TTT^AAA
5816 TTTAAA
EcoRI 288 GAATTC G^AATTC
5053 GAATTC
FbaI 1961 TGATCA T^GATCA
6126 TGATCA
Hin1I 5325 GGCGCC G[GA]^CG[CT]C
5597 GGCGCC
KasI 5325 GGCGCC G^GCGCC
5597 GGCGCC
MroI 855 TCCGGA T^CCGGA
3289 TCCGGA
NaeI 136 GCCGGC GCC^GGC
1666 GCCGGC
NarI 5325 GGCGCC GG^CGCC
5597 GGCGCC
NcoI 1458 CCATGG C^CATGG
2937 CCATGG
NgoMIV 136 GCCGGC G^CCGGC
1666 GCCGGC
PflMI 2211 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4852 CCATTTTGTGG
SapI 2234 GCTCTTC GCTCTTC(1/4)
5337 GCTCTTC
ScaI 2701 AGTACT AGT^ACT
3737 AGTACT
SfoI 5325 GGCGCC GGC^GCC
5597 GGCGCC
Van91I 2211 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4852 CCATTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvI BciVI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaBI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PmaCI PmlI PpuMI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI